That must take a long time. CombZ will stack
100 pictures in five or six minutes. Helicon
Focus (less configurable) is faster, but in
the case of the blue flowers it produced
strange halos in some places -- around very
dense areas for example. Having both programs
is useful for the stuff I do. When things
move, both produce artifacts. But should I
start injecting microscopy into this group?
Perhaps it would be out of place? But maybe
not as far out as religion?
Anyway here goes:
http://www.kolumbus.fi/mimosa/HOLD/401-452.jpg
this is a stack of 52 images in 2 um steps
taken at about 450X under phase contrast. The
object is the Lorica (siliceous skeleton) of
a small aquatic protist -- the name of which
escapes me for the moment but is irrelevant
anyway. This is a good example of how well
CombZ does it's job. The depth of focus
obtained is about 100 micrometres.
Don
Rob Studdert wrote:
On 14 Feb 2006 at 9:33, Don Williams wrote:
CombZ is free as you may know. Pick it up at:
http://www.hadleyweb.pwp.blueyonder.co.uk/CZ5/combinez5.htm
and let me know how you get on. Alan is one
of the real experts in this field.
Hi Don,
Thanks for the info, it sounds pretty impressive. To date I've simply been
using the panorama tool Hugin to resize, rotate, align the image stack WRT to a
nominated anchor image, which provides sub-pixel registration accuracy. I then
manually reveal the sharp areas of each overlaying image layer in PS.
Cheers,
Rob Studdert
HURSTVILLE AUSTRALIA
Tel +61-2-9554-4110
UTC(GMT) +10 Hours
[EMAIL PROTECTED]
http://members.ozemail.com.au/~distudio/publications/
Pentax user since 1986, PDMLer since 1998
--
Dr E D F Williams
__________________________________
http://www.kolumbus.fi/mimosa/index.htm
http://personal.inet.fi/cool/don.williams
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Updated: Added Print Gallery - 16 11 2005
