Apparently F5-refreshing the page reposts....I apologise!

Kenny

On Jan 28, 11:41 am, Kenny Daily <[email protected]> wrote:
> OK, so I re-ran my scripts double-checking everywhere that I used the
> genome from pygr.Data, and it still fails. So I worked up a test case,
> data and code follows. Before pickling, the _persistent_id is there.
> After pickling, its not.
>
> *** START test_genome.fa ***>test
>
> ACGCAGACTGACCTACGATCAAATAAGCCGAGCTAGCAAGCCCGCCGTAATCGATCGACGTACGTCGATCGATCGACCCC
> GAATAGACTCCGATAAGCGTAGTGTATATAGCGCGCCCGTATATAGGATGAGAAGAATATAAAGCTCCTCTCGAGATCGA
> *** END test_genome.fa ***
>
> *** START GENOME BUILD CODE ***
> from pygr import seqdb
> g = seqdb.BlastDB("/home/baldig/projects/genomics/nonsvn/results/yeast/
> Ty3/TEST/test_genome.fa")
> g.__doc__ = "Genome for testing Ty3 read clustering pipeline"
> import pygr.Data
> pygr.Data.getResource.addResource("Bio.Seq.Genome.TESTING.test_ty3",
> g)
> pygr.Data.save()
> *** END GENOME BUILD CODE ***
>
> *** START DATA CREATION CODE ***
> import cPickle
> import pygr.Data
> from pygr.sequence import Sequence
>
> # mimicks the data structure i'm using
> class HTS:
>     def __init__(self, seqs=[]):
>         self.seqs = seqs
>
> genome = pygr.Data.getResource("Bio.Seq.Genome.TESTING.test_ty3")
> foo = Sequence('CCCGCCGTAATCGATCGAC', 'foo')
> b = genome.blast(foo)
> s,d,e = b[foo].edges()[0]
>
> # check that persistent_id
> d.path.db._persistent_id
>
> H = HTS(seqs=[d])
> x = H.seqs[0]
>
> # check that persistent_id, again
> x.path.db._persistent_id
>
> Hs = {1: H}
> cPickle.dump(Hs, file("test_pickle_seqslice.pkl", "w"))
>
> *** END DATA CREATION CODE ***
>
> *** START DATA READ CODE ***
>
> import cPickle
> import pygr.Data
>
> class HTS:
>     def __init__(self, seqs=[]):
>         self.seqs = seqs
>
> d = cPickle.load(file("test_pickle_seqslice.pkl"))
> x = d[1]
> s = x.seqs[0]
>
> # I get <type 'exceptions.AttributeError'>: 'BlastDB' object has no
> attribute '_persistent_id'
> s.path.db._persistent_id
>
> *** END DATA READ CODE ***
>
> On Jan 28, 3:57 am, Christopher Lee <[email protected]> wrote:
>
> > On Jan 28, 2009, at 1:53 PM, Kenny Daily wrote:
>
> > > OK. These things make sense. However, I think what I'm doing is a
> > > little more complicated, and I've left out some of the important steps
> > > that may help explain. First, I'm sure that I'm using the pygr.Data
> > > object everytime...i.e. genome is always set by:
>
> > > genome = pygr.Data.getResource("Bio.Seq.Genome.YEAST.sacCer")
>
> > Kenny, could you check the c.sequence.path.db._persistent_id on the  
> > case from your example that gives the KeyError?  If this attribute is  
> > missing, the data was *not* loaded with a pygr.Data ID.  Let me know  
> > what you find.
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