Hi,

To build a pairwise assembly alignments, do I need to go through all the
steps as if I was building a msa?  Or, is there a simpler way to do this?
More specifically, can I skip the ref_assembly_self alignment?

BTW, I think there is a minor mistake on the
http://code.google.com/p/pygr/wiki/NlmsaFromAxtPairwise wiki page.

Shouldn't the seqlist be a seqdict?

# a dictionary of genomic sequences
seqdict = {'hg18':hg18, 'panTro2':panTro2, 'mm8':mm8, 'rn4':rn4,
'canFam2':canFam2}

for k, v in seqdict.iteritems():
    genomes[k] = seqdb.SequenceFileDB(v)

Best wishes,
Jenny

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