Hi,

I have constructed NLMSA files on a Linux station with success, but
the last step failed.
I checked the folder and file access mode, and have not seen anything
abnormal. I transfered the data on a Windows station where I obtained
the same error message.

Thanks for your help,

Michel



 *** WARNING: Unknown sequence oryCun1.scaffold_208340 ignored...
 *** WARNING: Unknown sequence panTro1.chr6_random ignored...
warning: non-alignment text lines ignored: prefix
, count 16190860
warning: non-alignment text lines ignored: prefix #, count 48
Index files saved.
Note: the NLMSA.seqDict was not saved to a file.
This is not necessary if you intend to save the NLMSA to pygr.Data.
But if you wish to open this NLMSA independently of pygr.Data,
you should call NLMSA.save_seq_dict() to save the seqDict info to a
file,
or in the future pass the saveSeqDict=True option to NLMSA.build().

To turn off this message, use the verbose=False option


>>> msa.build(saveSeqDict=True)
Traceback (most recent call last):
  File "<stdin>", line 1, in ?
  File "cnestedlist.pyx", line 1992, in pygr.cnestedlist.NLMSA.build
ValueError: not opened in write mode
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