Sorry, just created an issue on the google code page.

http://code.google.com/p/pygr/issues/detail?id=97

On Jun 8, 3:18 pm, Kenny Daily <[email protected]> wrote:
> Any idea how/what this error means, or how one would get it? Using a
> different sequence slice on the same chromome works fine. These
> datasets are built directly from the UCLA biodb server. Thanks!
>
> In [1]: import pygr.Data
> In [2]: msa = pygr.Data.getResource("Bio.MSA.UCSC.dm3_multiz15way")
> In [3]: genome = pygr.Data.getResource("Bio.Seq.Genome.DROME.dm3")
> In [4]: seq = -genome['chr3L'][10959977:10959996]
> In [5]: seq
> Out[5]: -chr3L[10959977:10959996]
>
> In [6]: print seq
> ------> print(seq)
> CATCCTGGTGACCCTGCTT
>
> In [7]: msa[seq]
> ---------------------------------------------------------------------------
> IOError                                   Traceback (most recent call
> last)
>
> /home/baldig/projects/genomics/nonsvn/output/fly/motifsearch/
> 2009_6_4_10_47_13_3_155_1/<ipython console> in <module>()
>
> /home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
> python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
> cnestedlist.so in pygr.cnestedlist.NLMSA.__getitem__()
>
> /home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
> python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
> cnestedlist.so in pygr.cnestedlist.NLMSASlice.__cinit__()
>
> /home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
> python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
> cnestedlist.so in pygr.cnestedlist.IntervalFileDBIterator.loadAll()
>
> /home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
> python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
> cnestedlist.so in pygr.cnestedlist.IntervalFileDBIterator.nextBlock()
>
> IOError: Iterator has no database!  Please provide a db argument.
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