For your described needs, you should not be using "render" multiple times. 
 Mako supports the following:

* Defs and Blocks - http://docs.makotemplates.org/en/latest/defs.html
* Template Inheritance 
- http://docs.makotemplates.org/en/latest/inheritance.html
* Template Namespace and 
Inclusion: http://docs.makotemplates.org/en/latest/namespaces.html

Other templating systems offer similar hooks.  You should, ideally, handle 
all rendering within the templating framework.

IMHO, you should avoid invoking `render` more than once in a given request 
unless you are very familiar/advanced with Pyramid.  Calls to `render` are 
hooked into Pyramid's events system (via the BeforeRender event 
subscriber), so you can trigger unintended consequences.

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