I have a dimeric protein 3HHZ.....to generate the dimer foll transformations
have to be made....the problem is that this is an identity matrix,so will
not change anything...can anyone help with this??

how can i get the asymmetric unit to generate dimer??


REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B

REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000

REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000

REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000

On Fri, Feb 25, 2011 at 6:40 PM, Hongbo Zhu <hongbo....@biotec.tu-dresden.de
> wrote:

> Hi, Kanika,
>
> are you looking for biological units of proteins when you say stable dimer?
> If this is the case, I recommend the page:
>
> http://pdbwiki.org/index.php/Biological_unit
> at the bottom of the page you can find four very useful servers for the
> determination of biological units of proteins (PQS is not updated anymore):
>    The Protein Quaternary Structure Server (PQS) [2] or [3]
>    The Macro-Molecular Structure Database (MSD) [4] or [5]
>    The Protein Interfaces, Surfaces and Assemblies server (Pisa) [6]
>    Protein quaternary structure investigation (PiQSi) [7]
>
> As a matter of fact, the PDB also provides the biological assembly of each
> PDB entry for download. More information can be found at:
>
> http://www.rcsb.org/pdb/static.do?p=education_discussion/Looking-at-Structures/bioassembly_tutorial.html
>
> If your protein is not from the PDB, you can still try pisa (
> http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html ), which accepts
> protein coordinate files uploaded by the users and determines the "stable
> dimer" or else-mer of your protein.
>
> hope these help!
> hongbo
>
>
> On 02/25/2011 01:46 PM, kanika sharma wrote:
>
>> Hi,
>> i am working to generate a dimer of my protein..I have made a duplicate
>> of my protein....Can any one tell me how to rotate my molecule to get
>> maximum stability..???
>>
>>
>> Regards..
>> Kanika
>>
>>
>>
>>
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>
> --
> Hongbo ZHU
> Postdoctoral Researcher
> Structural Bioinformatics
>
> Technische Universität Dresden
> Biotechnology Center
> Tatzberg 47/49
> 01307 Dresden, Germany
>
> Tel.: +49 (0) 351 463-40083
> Fax: +49 (0) 351 463-40087
> E-Mail: hongbo....@biotec.tu-dresden
> Webpage: www.biotec.tu-dresden.de
>
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