Dear Tsjerk and Hongbo,

Thank you very much for giving me ideas.

As mentioned by Honbo, I think shifting strand can work in only case
of very planar strand.

I'll try painting strands with GIMP -- thank you for your kindness!


Keitaro

2011/3/10 Hongbo Zhu <[email protected]>:
> I was also thinking along that line :) But that is pretty trick for a
> structure like beta-barrel, since you have to actually shrink the barrel
> toward the center.
>
> here is another hack that might work:
>
> 1. render only the black outline of the cartoon representation of your
> protein:
> set ray_trace_mode,2  # see http://www.pymolwiki.org/index.php/Ray
>
> 2. fill the cartoon, especially the beta strands using different colors
> using some image manipulation program (a great example is GIMP). This is
> fairly easy as most of such programs provide a "Fill Tool" (in GIMP it
> is the bucket button). Using such a tool you have the picture in a few
> clicks. I have actually made an example of a beta barrel (1opfA):
>
> http://www.biotec.tu-dresden.de/~hongboz/pymol_example/1opfA.html
>
> (In this quick example, I have oriented the protein such that both sides
> of some strands can be seen.)
>
> hongbo
>
>
> On 03/09/2011 04:03 PM, Tsjerk Wassenaar wrote:
>> Hi Keitaro,
>>
>> You can try to create a copy from the piece containing the sheet,
>> colour it differently, and change the coordinates slightly. It's a bit
>> tricky maybe, especially if you've got quite a bit of curvature.
>>
>> Hope it helps,
>>
>> Tsjerk
>>
>> On Wed, Mar 9, 211 at 3:22 PM, Keitaro Yamashita
>> <[email protected]>  wrote:
>>> Hi,
>>>
>>> Yes, there're definitely a few (?) exceptional cases.
>>> But maybe people don't use this feature in such case.
>>>
>>> Anyway, this feature is not included in current PyMOL, right?
>>> Then, is it hard to implement this feature (many changes in code required)?
>>>
>>> Keitaro
>>>
>>> 2011/3/8 gilleain torrance<[email protected]>:
>>>> Hi,
>>>>
>>>> Although most of the time the strands in a sheet have clear 'sides',
>>>> this won't always be true. Keitaro already mentioned the possibility
>>>> of twisted strands, but you can also get two sheets that share the
>>>> 'same' long strand - well, depending on the SSE assignment algorithm,
>>>> I suppose.
>>>>
>>>> Jellyrolls (http://www.cathdb.info/cathnode/2.60.120) are an example
>>>> of this, I seem to remember, as they have long strands that cross from
>>>> one sheet to another. As the sheets are in a sandwich, the strand will
>>>> change side halfway through.
>>>>
>>>> This coloring scheme still sounds like it would be useful, but there
>>>> might be some odd visual effects, or an algorithm to take care of
>>>> corner cases.
>>>>
>>>> gilleain
>>>>
>>>> On Tue, Mar 8, 2011 at 10:12 AM, Hongbo Zhu
>>>> <[email protected]>  wrote:
>>>>> I like the idea about coloring tim-barrels.
>>>>>
>>>>> At the moment the secondary structures can be selected in PyMOL using
>>>>> selector "ss". I wonder whether it makes sense to label each strand with
>>>>> an index so we can select each strand individually, e.g.
>>>>>
>>>>> select ss s5 # select the 5th strand from the N-term
>>>>>
>>>>> and then one can flip the colors of the two "sides" of the strand in
>>>>> case it is not colored properly according to the user.
>>>>>
>>>>> hongbo
>>>>>
>>>>> On 03/08/2011 10:16 AM, Keitaro Yamashita wrote:
>>>>>> Dear Hongbo,
>>>>>>
>>>>>>>> For example, user can put something like light source and "front" is
>>>>>>>> defined as the side light hits.
>>>>>>>> (though it would be undefined If sheet plane is placed parallel to the 
>>>>>>>> ray..)
>>>>>>> and even the same strand can have half of it facing the light source and
>>>>>>> the other half turned opposite to the source.
>>>>>>
>>>>>> I think this definition is useful when color e.g. beta-barrel, but
>>>>>> definitely includes ambiguity.
>>>>>> Then, how about defining front side as the C-beta direction of
>>>>>> starting residue of sheet?
>>>>>>
>>>>>>
>>>>>> Cheers,
>>>>>>
>>>>>> Keitaro
>>>>>>
>>>>>> 2011年3月8日火曜日 Hongbo Zhu [email protected]:
>>>>>>> Hi, Keitaro,
>>>>>>>
>>>>>>> On 03/08/2011 03:11 AM, Keitaro Yamashita wrote:
>>>>>>>> Dear Hongbo,
>>>>>>>>
>>>>>>>> For example, user can put something like light source and "front" is
>>>>>>>> defined as the side light hits.
>>>>>>>> (though it would be undefined If sheet plane is placed parallel to the 
>>>>>>>> ray..)
>>>>>>> and even the same strand can have half of it facing the light source and
>>>>>>> the other half turned opposite to the source.
>>>>>>>>
>>>>>>>> But I think any definition is ok if front and back could be alternated
>>>>>>>> easily by user operation.
>>>>>>> sounds like an interesting operation, though.
>>>>>>>>
>>>>>>>>
>>>>>>>> Cheers,
>>>>>>>>
>>>>>>>> Keitaro
>>>>>>>>
>>>>>>>> 2011/3/8 Hongbo Zhu<[email protected]>:
>>>>>>>>> how do you define "front" and "back" sides of sheets in general?
>>>>>>>>>
>>>>>>>>
>>>>>>>> ------------------------------------------------------------------------------
>>>>>>>> What You Don't Know About Data Connectivity CAN Hurt You
>>>>>>>> This paper provides an overview of data connectivity, details
>>>>>>>> its effect on application quality, and explores various alternative
>>>>>>>> solutions. http://p.sf.net/sfu/progress-d2d
>>>>>>>> _______________________________________________
>>>>>>>> PyMOL-users mailing list ([email protected])
>>>>>>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>>>>>>> Archives: http://www.mail-archive.com/[email protected]
>>>>>>>
>>>>>>> --
>>>>>>> Hongbo ZHU
>>>>>>> Postdoctoral Researcher
>>>>>>> Structural Bioinformatics
>>>>>>>
>>>>>>> Technische Universität Dresden
>>>>>>> Biotechnology Center
>>>>>>> Tatzberg 47/49
>>>>>>> 01307 Dresden, Germany
>>>>>>>
>>>>>>> Tel: +49 (0) 351 463-40083
>>>>>>> Fax: +49 (0) 351 463-40087
>>>>>>> E-Mail:  hongbo.zhu at biotec
>>>>>>> Webpage: www.biotec.tu-dresden.de
>>>>>>>
>>>>>>> ------------------------------------------------------------------------------
>>>>>>> What You Don't Know About Data Connectivity CAN Hurt You
>>>>>>> This paper provides an overview of data connectivity, details
>>>>>>> its effect on application quality, and explores various alternative
>>>>>>> solutions. http://p.sf.net/sfu/progress-d2d
>>>>>>> _______________________________________________
>>>>>>> PyMOL-users mailing list ([email protected])
>>>>>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>>>>>> Archives: http://www.mail-archive.com/[email protected]
>>>>>>>
>>>>>>
>>>>>> ------------------------------------------------------------------------------
>>>>>> What You Don't Know About Data Connectivity CAN Hurt You
>>>>>> This paper provides an overview of data connectivity, details
>>>>>> its effect on application quality, and explores various alternative
>>>>>> solutions. http://p.sf.net/sfu/progress-d2d
>>>>>> _______________________________________________
>>>>>> PyMOL-users mailing list ([email protected])
>>>>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>>>>> Archives: http://www.mail-archive.com/[email protected]
>>>>>
>>>>> --
>>>>> Hongbo ZHU
>>>>> Postdoctoral Researcher
>>>>> Structural Bioinformatics
>>>>>
>>>>> Technische Universität Dresden
>>>>> Biotechnology Center
>>>>> Tatzberg 47/49
>>>>> 01307 Dresden, Germany
>>>>>
>>>>> Tel: +49 (0) 351 463-40083
>>>>> Fax: +49 (0) 351 463-40087
>>>>> E-Mail:  hongbo.zhu at biotec
>>>>> Webpage: www.biotec.tu-dresden.de
>>>>>
>>>>> ------------------------------------------------------------------------------
>>>>> What You Don't Know About Data Connectivity CAN Hurt You
>>>>> This paper provides an overview of data connectivity, details
>>>>> its effect on application quality, and explores various alternative
>>>>> solutions. http://p.sf.net/sfu/progress-d2d
>>>>> _______________________________________________
>>>>> PyMOL-users mailing list ([email protected])
>>>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>>>> Archives: http://www.mail-archive.com/[email protected]
>>>>
>>>> ------------------------------------------------------------------------------
>>>> What You Don't Know About Data Connectivity CAN Hurt You
>>>> This paper provides an overview of data connectivity, details
>>>> its effect on application quality, and explores various alternative
>>>> solutions. http://p.sf.net/sfu/progress-d2d
>>>> _______________________________________________
>>>> PyMOL-users mailing list ([email protected])
>>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>>> Archives: http://www.mail-archive.com/[email protected]
>>>
>>> ------------------------------------------------------------------------------
>>> Colocation vs. Managed Hosting
>>> A question and answer guide to determining the best fit
>>> for your organization - today and in the future.
>>> http://p.sf.net/sfu/internap-sfd2d
>>> _______________________________________________
>>> PyMOL-users mailing list ([email protected])
>>> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
>>> Archives: http://www.mail-archive.com/[email protected]
>>
>>
>>
>
> --
> Hongbo ZHU
> Postdoctoral Researcher
> Structural Bioinformatics
>
> Technische Universität Dresden
> Biotechnology Center
> Tatzberg 47/49
> 01307 Dresden, Germany
>
> Tel: +49 (0) 351 463-40083
> Fax: +49 (0) 351 463-40087
> E-Mail:  hongbo.zhu at biotec
> Webpage: www.biotec.tu-dresden.de
>
> ------------------------------------------------------------------------------
> Colocation vs. Managed Hosting
> A question and answer guide to determining the best fit
> for your organization - today and in the future.
> http://p.sf.net/sfu/internap-sfd2d
> _______________________________________________
> PyMOL-users mailing list ([email protected])
> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users
> Archives: http://www.mail-archive.com/[email protected]

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