They are different types of maps.

2F67 -- X-ray map
7B5C -- EM map

7B5C is not a 2fofc map.

Best wishes,
Zuben
________________________________
From: Criss Hartzell <criss.hartz...@gmail.com>
Sent: Wednesday, October 27, 2021 2:51 PM
To: Pymol User list <pymol-users@lists.sourceforge.net>
Subject: [EXTERNAL] [PyMOL] basic trouble viewing electron density maps

I am having trouble viewing electron density maps.
Everything works fine if I use the protein 2F67. But with 7B5C, I am stumped.

fetch 7b5c, type=2fofc gives me an error:

Warning: failed to fetch from 
https://www.ebi.ac.uk/pdbe/coordinates/files/7b5c.ccp4<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ebi.ac.uk_pdbe_coordinates_files_7b5c.ccp4&d=DwMFaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=8AY7h2N3TQ7k7n9RDhxT46t0ViqfY3ViGGLPrQZPtGw&m=gjXm-pi8KE6bTBC8di1Ue68XZy2E2aRL9MnN2J1bJLc&s=MUITh2JiB3m_JBC4jNMoDm8CR051F-3qt8WIJbFOHD4&e=>
 Error-fetch: unable to load '7b5c'.

If I go to EMDB and download the map file (EMD_12025) and load it manually:

(set normalize_ccp4_maps, 0

load H:/.../emd_12025.map, \emd_12025

isomesh emd_12025_isomesh, emd_12025, 1.0)

The screen is blank and changing levels, etc. does nothing.

But it works in UCSF Chimera. What am I missing?

Thanks.

Criss


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