Hi Tanaya, You can replace the default B-factor values in a PDB file with user defined values.
Check out these links. They show how to use values from a user-defined file and set a color gradient on your protein: 1. https://pymolwiki.org/index.php/Load_new_B-factors 2. https://pymolwiki.org/index.php/Color#Reassigning_B-Factors_and_Coloring Hope this helps. All the best. Best regards, ----------------------------------------------------- *Dr Dheeraj Prakaash* Postdoctoral Research Associate Syma Khalid Research Group <https://symakhalidresearchgroup.wordpress.com/> Department of Biochemistry <https://www.bioch.ox.ac.uk/> University of Oxford <https://www.ox.ac.uk/> Web: LinkedIn <https://www.linkedin.com/in/dheeraj-prakaash-b757679a/> | Twitter <https://twitter.com/DJ_Biophys> | ResearchGate <https://www.researchgate.net/profile/Dheeraj-Prakaash> -----------------------------------------------------
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