Hi relax-users, dear Edward, we use methanol temperature calibration to make sure the relaxation measurements are taking place at the same temperature. Interestingly, I see significantly diverting signals in my HSQCs / TROSYs which I believe are due to temperature differences. I don't see diverging signals between T1, T2 and Hetero-NOE measurements, so at least the data at one field seem to be consistent in any case.
One strategy to overcome this was to * record a HSQC/TROSY at different temperatures at the different spectrometers (conveniently by using Brukers multi_zgvt), * reference the two sets of spectra to a residue signal which seems to be at exactly the same position regardless of temperature * select the temperature where the NH spectra are nearly 100% identical What is your experience with calibration strategies? Do you see any diverging NH signals at different magnets? Cheers and a happy new year, Martin _______________________________________________ relax (http://www.nmr-relax.com) This is the relax-users mailing list [email protected] To unsubscribe from this list, get a password reminder, or change your subscription options, visit the list information page at https://mail.gna.org/listinfo/relax-users
