Hi,

I wanted to know if there is a way to have the exact counts of reads supporting the reference and alternate alleles because that's weird, the reference alleles can be tagged 0 !
Here is an example of my file :

scaffold16|size214105 154542 . A G 487 . DP=72;VDB=0.5037;SGB=27.7261;MQSB=0.972745;MQ0F=0;AC=4;AN=4;DP4=0,0,33,31;MQ=58 GT:PL ./.:0,0,0 1/1:255,57,0
1/1:255,135,0
scaffold16|size214105 168573 . T C 487 . DP=75;VDB=0.347592;SGB=32.578;MQSB=0.890283;MQ0F=0;AC=4;AN=4;DP4=0,0,30,43;MQ=59 GT:PL ./.:0,0,0 1/1:255,
63,0    1/1:255,157,0

I would like to have the sum of my 4 columns of DP4 = DP, is it a way to do that ?

Thanks,

Jeanne





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