To samtools-help,

Is this list also for questions related to bcftools?

The documentation for the tool PLINK lists the sequence in which command line 
flags are executed:
https://www.cog-genomics.org/plink2/order

Is there a similar page for bcftools?

The reason I'm asking, is because I'm filtering out samples and then filtering 
based on AF.

1) Can I use either of the two methods below?
2) Will the AF be calculated for the filtered samples only? Or is the AF 
evaluated prior to sample filtering?

bcftools view --exclude AF<0.1
bcftools view --max-af 0.1

Thank you very much.

Tommy



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