And no, sorry, I am afraid we do not have this documented.

petr

On Tue, 2016-10-04 at 16:07 +0200, Petr Danecek wrote:
> Hi,
> 
> if I remember correctly the -i/-e options are applied before and
> --max-af after subsetting. If unsure, or if a different behaviour is
> needed, it is possible to stream multiple commands to make it explicit:
> 
> $bt view -e .. -Ou | $bt view -s ..
> 
> petr
> 
> 
> On Tue, 2016-10-04 at 13:56 +0000, Tommy Carstensen wrote:
> > To samtools-help,
> > 
> > 
> > Is this list also for questions related to bcftools?
> > 
> > 
> > The documentation for the tool PLINK lists the sequence in which
> > command line flags are executed:
> > https://www.cog-genomics.org/plink2/order
> > 
> > 
> > Is there a similar page for bcftools?
> > 
> > 
> > The reason I'm asking, is because I'm filtering out samples and then
> > filtering based on AF.
> > 
> > 
> > 1) Can I use either of the two methods below?
> > 2) Will the AF be calculated for the filtered samples only? Or is the
> > AF evaluated prior to sample filtering?
> > 
> > 
> > bcftools view --exclude AF<0.1
> > bcftools view --max-af 0.1
> > 
> > 
> > Thank you very much.
> > 
> > 
> > Tommy
> 




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