Dear Sir/Madam, I met a problem while running samtools faidx command. It shows like:
$ cd /biotool/samtools-1.3.1 $ ./samtools faidx /data/hg19.fasta [fai_build] fail to open the FASTA file /data/hg19.fasta Could not build fai index /data/hg19.fasta.fai I tried 3 reference genomes respectively: hg19.fasta, ucsc.hg19.fasta and hg19.fa. They all show error messages like above. However, if I use one single chromosome reference , like chrX.fa, instead of the whole hg19 reference, faidx command goes well and no error message appears. Is there any way to fix that? I would be very appreciate if you reply me. CHEN Ran Sequencing Superviser Molecular Biology Laboratory Wuhan Kindstar Diagnostics Co., Ltd. Kindstar Global (Beijing) Medical Technology Co., Ltd. Mail: chen...@kindstar.com.cn<mailto:chen...@kindstar.com.cn> Tel: (86) 027-85573007-8413 Phone: 18502711615 LinkedIn: https://cn.linkedin.com/in/chromochen/en
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