Dear samtools support,
I'm doing my master's thesis in molecular ecology on bristletails. I build a
transcriptome out of 17 individuals of Machilis pallida and now I want to
perform a SNP calling using samtools/bcftools.
As there is no reference genome available, can I use my transcriptome as a
reference for the analysis?
I can't find any answer to this question and would be very glad for any help.
Kind regards,
Marlene Haider
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