On 10 Mar 2021, at 21:04, Remik Ziemlinski <rziemlin...@snapgene.com> wrote: > The attached file worked with version 1.9, but is failing with 1.10 and 1.11. > Is the file valid? Is there a regression after v1.9? > [...] > # output from version 1.9 > @HD VN:1.6 SO::unsorted > @SQ SN:reference LN:5495
Your @HD line is delimited by spaces instead of tabs, which is invalid. The extra colon is not exactly helping either: it's syntactically valid and can be read in fine, but the value of ":unsorted" will be considered invalid by anything that actually looks at the SO value. John _______________________________________________ Samtools-help mailing list Samtools-help@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/samtools-help