:* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data was obtain from a co-crystal and the wilson B
factor
of the data is about 70
Dear All:
Thank you for help. Now I will make a summary of this topic.
First, the actual situation is that:
1. Protein and peptide have a affinity about 10E-7 to 10E-8 molar.
2. The 2.6 angstrom data were got from a co-crystal and had a wilson B about
70, in the experiment protein/peptide have a
: +33 (0)3 88 41 70 02
[EMAIL PROTECTED]
-Message d'origine-
*De :* CCP4 bulletin board [mailto: [EMAIL PROTECTED] la part de*Jiamu Du
*Envoyé :* lundi 30 avril 2007 05:57
*À :* CCP4BB@JISCMAIL.AC.UK
*Objet :* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex
:* lundi 30 avril 2007 05:57
*À :* CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
*Objet :* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data was obtain from a co-crystal
high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data was obtain from a co-crystal and the wilson B factor
of the data is about 70.
The affinity between protein and peptide is about 10E-7
:* CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
*Objet :* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data was obtain from a co-crystal and the wilson B factor
*? :* CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
*Objet :* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data was obtain from a co-crystal and the wilson B
mailto:CCP4BB@JISCMAIL.AC.UK mailto:CCP4BB@JISCMAIL.AC.UK
mailto:CCP4BB@JISCMAIL.AC.UK
*Objet :* [ccp4bb] extra high B factor
Dear All:
I am refining a protein-peptide complex struture at 2.6
angstrom resolution.
The data
: Montag, 30. April 2007 16:32
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] extra high B factor
Dear all,
Frankly speaking I am having some doubts about this whole discussion.
1. Apparently, it does not make a difference in R and Rfree
whether the peptide is in the structure or not. This does
If you assume atomic B-factors of 100 or larger and
calculate the density, I really can't see how this is possible.
Well, I do. The recipe has been published in NSB as a cautionary tale.
http://www.ruppweb.org/cvs/br/rupp_2001_NSB_questions_BotA.pdf
and there is quite fail-safe away to
I will try it soon. If it work, I will paste a sumary.
Thanks
On 4/30/07, Jacqueline Vitali [EMAIL PROTECTED] wrote:
Hello,
I think it is because the peptide does not have full occupancy. I do
not know if it is OK to fix occupancy to 0.5 and do the refinement.
That would bring temp factor
Hi Jiamu,
is the high B-value of your protein due to motions, which are not
modeled appropiately ? Which program by the way are you using for
refinement ? Then the TLSMD server might help you here. Monomer or
multimer in the asu ? NCS used, if so checked that they actually follow
NCS and
12 matches
Mail list logo