[ccp4bb] Beamtime @ SLS
=== SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS === Proposal application deadline: Wednesday, June 15, 2011 Periods: September 1, 2011 - December 31, 2011 (Normal / Test proposals) September 1, 2011 - August 31, 2012 (Long-term proposals) Proposal submission: http://www.psi.ch/sls/px-beamlines-call-for-proposals Travel support: http://www.psi.ch/useroffice/sls-elisa PSI DUO application for iphone: http://itunes.apple.com/ch/app/psi-duo/id375328818?mt=8 News: - PILATUS detector runs two times faster (25 Hz vs. 12.5 Hz) at X06SA, which offers faster fine-phi slicing data collection. - X10SA accepts proposals for combined single crystal diffraction and UV-Vis, fluorescence and Raman spectroscopy studies (R. Owen, et al., JSR, 2009, 16, 173) - In-situ X-ray diffraction screening in user operation at X06DA with any SBS format plate (R. Bingel-Erlenmeyer, et al., Crystal Growth Design 2011, 11, 916) - Automatic sample changer (IRELEC CATS) with SPINE pucks, vials, and caps (MOLECULAR DIMENSIONS) available at all beamlines (except on the MD2 station). X06SA Beamline features (http://www.psi.ch/sls/pxi/pxi) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 Å) - Focused beam size and divergency: HRD - 85x10 microns and 0.35x0.06 mrad; MD2 - 25x5 microns and 0.5x0.4 mrad - PILATUS 6M pixel detector at the High Resolution Diffractometer, allowing continuous, fine phi-sliced data acquisition (25 frames per second) with 20 bit dynamic range (see http://pilatus.web.psi.ch/ or www.dectris.com for further information) - MAR225 CCD at Micro-Diffractometer MD2, allowing data collection with a focussed beam size of 25 x 5 micrometers, and down to 10 x 5 micrometer using an aperture. X06DA Beamline features (http://www.psi.ch/sls/pxiii/pxiii) - Super-bending magnet beamline with flux of 5x10¹¹ photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 17.5 keV (2.07 - 0.71 Å) - Focused beam size and divergency: 80x45 microns and 2x0.5 mrad (with possibility to reduce horizontal divergency to 0.4 mrad) - Mini-hutch design for fast manual mounting - MAR225 CCD detector - In-situ X-ray diffraction screening (with any SBS format plate) available during users shifts (R. Bingel-Erlenmeyer, et al., Crystal Growth Design 2011, 11, 916) X10SA Beamline features (http://www.psi.ch/sls/pxii/pxii) - Undulator beamline with flux of 2x10^12 photons/sec at 12.4 keV (1Å) and fully tunable from 6.0 to 20 keV (2.07 - 0.62 Å) - Focused beam size and divergency: 50x10 microns and 0.6x0.1 mrad - In-situ on-axis single crystal UV-Vis, fluorescence, Raman and Resonance Raman microspectrophotometer (http://www.psi.ch/sls/pxii/spectrolab) - PILATUS 6M pixel detector Best regards, The MX group at SLS __ Meitian Wang Swiss Light Source at Paul Scherrer Institut CH-5232 Villigen PSI - http://www.psi.ch/sls/ Phone: +41 56 310 4175 Fax: +41 56 310 5292
[ccp4bb] PhD position
PhD studentship in biochemistry/structural biology, University of Nottingham We are looking for a highly motivated individual with a background in biochemistry or related discipline to join a project on the discovery and characterisation of deubiquitinating enzyme ligands. The aim of the project is to map the interaction with two known binding partners implicated in cervical cancer and to isolate novel binding peptides using phage display techniques. Identified peptides will be further characterised using biophysical methods and X-ray crystallography and their effect upon catalytic activity investigated. This will lead to a better understanding of substrate specificity and pave the way for the development of novel anti-cancer drugs. The student will have the opportunity to join a multidisciplinary team based in the new Centre for Biomolecular Sciences and the School of Veterinary Medicine and Science at the University of Nottingham, benefiting from state-of-the-art facilities and a stimulating research environment. The fully funded three year studentship commencing in autumn 2011 will pay tuition fees and a stipend for EU/UK students of around £13,590 per year. To apply for this studentship, please send a cover letter and curriculum vitae with the names of two referees to ingrid.drev...@nottingham.ac.ukmailto:ingrid.drev...@nottingham.ac.uk and kevin.go...@nottingham.ac.ukmailto:kevin.go...@nottingham.ac.uk. Ingrid Dreveny Centre for Biomolecular Sciences University of Nottingham University Park Nottingham, NG7 2RD United Kingdom Tel: +44 115 8468015 Fax: +44 115 8468002 http://www.nottingham.ac.uk/pharmacy/research/mcsb/structuralbiology/index.aspx This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
Re: [ccp4bb] Question about the statistical analysis-might be a bit off topic
The short answer can be found in item 2 in this link: http://science.widener.edu/svb/stats/error.html The long answer is I highly recommend Error Analysis by John Taylor: http://science.widener.edu/svb/stats/error.html If you can find the first edition (which can fit in your pocket) then consider yourself lucky. Later editions suffer book bloat. James On Jun 4, 2011, at 10:44 AM, capricy gao wrote: If means and standard deviations of A and B are known, how to estimate the variance of A/B? Thanks.
