[ccp4bb] CrystalClear tape rolls
Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij
Re: [ccp4bb] CrystalClear tape rolls
Hi Mark, last time I checked (few months back), Hampton still sold tape. Shipping to Europe is extortionate but if you combine with screens and other goodies, it's just about bearable. Andreas On 01/12/2014 10:24, Mark J van Raaij wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij -- Andreas Förster X-ray Crystallography Facility Manager Centre for Structural Biology Imperial College London
Re: [ccp4bb] CrystalClear tape rolls
you are right, I must have been looking with my ears when I checked this morning. https://hamptonresearch.com/product_detail.aspx?cid=10sid=88pid=271 Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 1 Dec 2014, at 12:05, Andreas Förster wrote: Hi Mark, last time I checked (few months back), Hampton still sold tape. Shipping to Europe is extortionate but if you combine with screens and other goodies, it's just about bearable. Andreas On 01/12/2014 10:24, Mark J van Raaij wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij -- Andreas Förster X-ray Crystallography Facility Manager Centre for Structural Biology Imperial College London
[ccp4bb] how to dump diffraction image header info?
Dear all, I'd like to dump header information of a diffraction image file. CCP4 diffdump is a good tool, though it outputs the same angle for both oscillation start and end for .cbf image. (bug?) Are there any other tools to get diffraction image header info? Regards - Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp) Discovery Research Dept. (Biostructure Gr.) Chugai Pharmaceutical Co.,Ltd.
Re: [ccp4bb] how to dump diffraction image header info?
Dear Takaaki, dxtbx provides a way to do this (see http://journals.iucr.org/j/issues/2014/04/00/jo5001/jo5001.pdf). It is available in ccp4-python (try 'import dxtbx'), however there is no script currently in CCP4 that uses this to print the header information. One could write such a script easily though, and I would be happy to provide one off-list if you are interested. Scripts like this already exist in the DIALS project (http://dials.sourceforge.net/ - try e.g. dials.import /my/images/*.cbf; dials.show_models datablock.json). Note that the dxtbx way is not giving you direct access to header values, but will print information about experimental models constructed from the headers. Usually this is what you want. If not, ADXV ( http://www.scripps.edu/tainer/arvai/adxv.html) is a useful tool that can display image header values, but I don't know if this can be scripted to print to console. Cheers -- David On 1 December 2014 at 11:22, Takaaki Fukami fukami...@chugai-pharm.co.jp wrote: Dear all, I'd like to dump header information of a diffraction image file. CCP4 diffdump is a good tool, though it outputs the same angle for both oscillation start and end for .cbf image. (bug?) Are there any other tools to get diffraction image header info? Regards - Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp) Discovery Research Dept. (Biostructure Gr.) Chugai Pharmaceutical Co.,Ltd.
[ccp4bb] AW: how to dump diffraction image header info?
Dear Takaaki, The .cbf images I work with (SLS) have their header as plain text in front of the image. With an editor like vi I can examine the contents. Also with the more command you can have a look at it. What I find in the header though, is Start_angle and Angle_increment; no end angle. The command typed in a terminal window: grep -a ngle imagename.cbf will give you this information. Best, Herman -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Takaaki Fukami Gesendet: Montag, 1. Dezember 2014 12:22 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] how to dump diffraction image header info? Dear all, I'd like to dump header information of a diffraction image file. CCP4 diffdump is a good tool, though it outputs the same angle for both oscillation start and end for .cbf image. (bug?) Are there any other tools to get diffraction image header info? Regards - Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp) Discovery Research Dept. (Biostructure Gr.) Chugai Pharmaceutical Co.,Ltd.
Re: [ccp4bb] how to dump diffraction image header info?
Hi Takaaki The easy way, since the headers of CBF images are plain ASCII, is to check your image with a text viewer (e.g. more or less on the command-line). This will also work for ADSC SMV format, Rigaku d*Trek format, Bruker 86 100 formats, but not for Rigaku original R-Axis format, Agilent Oxford format, or for MarIP or Rayonix CCD. Or you could use Mosflm or iMosflm, since these read the information and print out most of the useful stuff. On 1 Dec 2014, at 12:35, David Waterman wrote: Dear Takaaki, dxtbx provides a way to do this (see http://journals.iucr.org/j/issues/2014/04/00/jo5001/jo5001.pdf). It is available in ccp4-python (try 'import dxtbx'), however there is no script currently in CCP4 that uses this to print the header information. One could write such a script easily though, and I would be happy to provide one off-list if you are interested. Scripts like this already exist in the DIALS project (http://dials.sourceforge.net/ - try e.g. dials.import /my/images/*.cbf; dials.show_models datablock.json). Note that the dxtbx way is not giving you direct access to header values, but will print information about experimental models constructed from the headers. Usually this is what you want. If not, ADXV (http://www.scripps.edu/tainer/arvai/adxv.html) is a useful tool that can display image header values, but I don't know if this can be scripted to print to console. Cheers -- David On 1 December 2014 at 11:22, Takaaki Fukami fukami...@chugai-pharm.co.jp wrote: Dear all, I'd like to dump header information of a diffraction image file. CCP4 diffdump is a good tool, though it outputs the same angle for both oscillation start and end for .cbf image. (bug?) Are there any other tools to get diffraction image header info? Regards - Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp) Discovery Research Dept. (Biostructure Gr.) Chugai Pharmaceutical Co.,Ltd. Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing)
Re: [ccp4bb] how to dump diffraction image header info?
One can use the strings command to show the header: strings myimage.cbf Thierry -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of herman.schreu...@sanofi.com Sent: Monday, December 01, 2014 7:40 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: how to dump diffraction image header info? Dear Takaaki, The .cbf images I work with (SLS) have their header as plain text in front of the image. With an editor like vi I can examine the contents. Also with the more command you can have a look at it. What I find in the header though, is Start_angle and Angle_increment; no end angle. The command typed in a terminal window: grep -a ngle imagename.cbf will give you this information. Best, Herman -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Takaaki Fukami Gesendet: Montag, 1. Dezember 2014 12:22 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] how to dump diffraction image header info? Dear all, I'd like to dump header information of a diffraction image file. CCP4 diffdump is a good tool, though it outputs the same angle for both oscillation start and end for .cbf image. (bug?) Are there any other tools to get diffraction image header info? Regards - Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp) Discovery Research Dept. (Biostructure Gr.) Chugai Pharmaceutical Co.,Ltd. Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
[ccp4bb] pMAL -p2X vector
Dear CCP4Sorry for the question, does anyone work with the pMAL-p2X vector not c2X, because I am trying to find this vector for a long time now, and neither NEB of life technologies provide this vector. Does anyone know from where I can get it or any company provides it, I would be grateful. Best RegardsRana
Re: [ccp4bb] how to dump diffraction image header info?
Dear All,
dxtbx.print_header /path/to/image should work
Graemes-MacBook-Pro:~ graeme$ dxtbx.print_header
data/i04-BAG-training/th_8_2_0001.cbf
=== data/i04-BAG-training/th_8_2_0001.cbf ===
Using header reader: FormatCBFMiniPilatusDLS6MSN100
Beam:
wavelength: 0.97625
sample to source direction : {0,0,1}
divergence: 0
sigma divergence: 0
polarization normal: {0,1,0}
polarization fraction: 0.999
Goniometer:
Rotation axis: {1,0,0}
Fixed rotation: {1,0,0,0,1,0,0,0,1}
Setting rotation:{1,0,0,0,1,0,0,0,1}
Detector:
Panel:
pixel_size:{0.172,0.172}
image_size: {2463,2527}
trusted_range: {-1,161977}
fast_axis: {1,0,0}
slow_axis: {0,-1,0}
origin: {-210.76,205.277,-265.27}
Scan:
image range: {1,1}
oscillation: {82,0.15}
Total Counts: 1437712
Best wishes Graeme
On Mon Dec 01 2014 at 12:40:09 PM David Waterman dgwater...@gmail.com
wrote:
Dear Takaaki,
dxtbx provides a way to do this (see
http://journals.iucr.org/j/issues/2014/04/00/jo5001/jo5001.pdf). It is
available in ccp4-python (try 'import dxtbx'), however there is no script
currently in CCP4 that uses this to print the header information. One could
write such a script easily though, and I would be happy to provide one
off-list if you are interested. Scripts like this already exist in the
DIALS project (http://dials.sourceforge.net/ - try e.g. dials.import
/my/images/*.cbf; dials.show_models datablock.json).
Note that the dxtbx way is not giving you direct access to header values,
but will print information about experimental models constructed from the
headers. Usually this is what you want. If not, ADXV (
http://www.scripps.edu/tainer/arvai/adxv.html) is a useful tool that can
display image header values, but I don't know if this can be scripted to
print to console.
Cheers
-- David
On 1 December 2014 at 11:22, Takaaki Fukami fukami...@chugai-pharm.co.jp
wrote:
Dear all,
I'd like to dump header information of a diffraction image file.
CCP4 diffdump is a good tool, though it outputs the same angle
for both oscillation start and end for .cbf image. (bug?)
Are there any other tools to get diffraction image header info?
Regards
-
Takaaki Fukami (mailto:fukami...@chugai-pharm.co.jp)
Discovery Research Dept. (Biostructure Gr.)
Chugai Pharmaceutical Co.,Ltd.
Re: [ccp4bb] CrystalClear tape rolls
Hi Mark, I recently ordered HD Clear Sealing Tape, 3 inches wide from Jena: http://www.jenabioscience.com/cms/en/1/catalog/287_sealing_tape.html Best, Chris On Mon, Dec 1, 2014 at 12:18 PM, Mark J van Raaij mjvanra...@cnb.csic.es wrote: you are right, I must have been looking with my ears when I checked this morning. https://hamptonresearch.com/product_detail.aspx?cid=10sid=88pid=271 Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 1 Dec 2014, at 12:05, Andreas Förster wrote: Hi Mark, last time I checked (few months back), Hampton still sold tape. Shipping to Europe is extortionate but if you combine with screens and other goodies, it's just about bearable. Andreas On 01/12/2014 10:24, Mark J van Raaij wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij -- Andreas Förster X-ray Crystallography Facility Manager Centre for Structural Biology Imperial College London
Re: [ccp4bb] CrystalClear tape rolls
… and there is RigakuReagents, formerly EmeraldBio, too: https://www.rigakureagents.com/p-595-crystallization-plates.aspx https://www.rigakureagents.com/p-595-crystallization-plates.aspx Or google for HD clear duck tape. Several office supply retailers in the US sell it. Cheers, Jan -- Jan Abendroth Emerald BioStructures Seattle / Bainbridge Island WA, USA home: Jan.Abendroth_at_gmail.com work: JAbendroth_at_embios.com http://www.emeraldbiostructures.com On Dec 1, 2014, at 6:21 AM, Chris Fage cdf...@gmail.com wrote: Hi Mark, I recently ordered HD Clear Sealing Tape, 3 inches wide from Jena: http://www.jenabioscience.com/cms/en/1/catalog/287_sealing_tape.html http://www.jenabioscience.com/cms/en/1/catalog/287_sealing_tape.html Best, Chris On Mon, Dec 1, 2014 at 12:18 PM, Mark J van Raaij mjvanra...@cnb.csic.es mailto:mjvanra...@cnb.csic.es wrote: you are right, I must have been looking with my ears when I checked this morning. https://hamptonresearch.com/product_detail.aspx?cid=10sid=88pid=271 https://hamptonresearch.com/product_detail.aspx?cid=10sid=88pid=271 Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 tel:%28%2B34%29%2091%20585%204616 http://www.cnb.csic.es/~mjvanraaij http://www.cnb.csic.es/~mjvanraaij On 1 Dec 2014, at 12:05, Andreas Förster wrote: Hi Mark, last time I checked (few months back), Hampton still sold tape. Shipping to Europe is extortionate but if you combine with screens and other goodies, it's just about bearable. Andreas On 01/12/2014 10:24, Mark J van Raaij wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 tel:%28%2B34%29%2091%20585%204616 http://www.cnb.csic.es/~mjvanraaij http://www.cnb.csic.es/~mjvanraaij -- Andreas Förster X-ray Crystallography Facility Manager Centre for Structural Biology Imperial College London
[ccp4bb] cryoprotectant
Hi, Has anyone used citrate as the sole cryoprotectant? If so, what concentration was needed? Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 www.khayatlab.org
Re: [ccp4bb] pMAL -p2X vector
Dear Rana, just to point your attention and that of the fellow BB members to a collection of plasmids (the pCri System) we have prepared and compiled. They should be available very soon to everybody via Addgene. Best, Xavier http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0112643 On Mon, Dec 1, 2014 at 2:23 PM, rana ibd 0285de88044a-dmarc-requ...@jiscmail.ac.uk wrote: Dear CCP4 Sorry for the question, does anyone work with the pMAL-p2X vector not c2X, because I am trying to find this vector for a long time now, and neither NEB of life technologies provide this vector. Does anyone know from where I can get it or any company provides it, I would be grateful. Best Regards Rana -- F. Xavier Gomis-Rüth *Research Professor CSIC* Proteolysis Lab Department of Structural Biology Molecular Biology Institute of Barcelona, *Vice-Director* Spanish Research Council CSIC Barcelona Science Park, Helix Building c/ Baldiri Reixac,15-21 *08028 Barcelona (Spain*) --- Phone:+34-934 020 186. Fax:+34-934 034 979 e-mail: xgr...@ibmb.csic.es Lab-Homepage: www.ibmb.csic.es/home/xgomis
Re: [ccp4bb] cryoprotectant
Hi Reza, 1.0 and 1.25 M citrate are potential cryo solutions. They still had faint ice rings present but were not dominant. It is also difficult to make a solution more concentrated then 1.75 M citrate. Hope this helps. -Adam On Dec 1, 2014, at 10:59 AM, Reza Khayat rkha...@ccny.cuny.edu wrote: Hi, Has anyone used citrate as the sole cryoprotectant? If so, what concentration was needed? Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 www.khayatlab.org
[ccp4bb] Looking for supplier for 'Q' crystallization plates.
Hi all, This plate used to be the Q plate (HR3-124) from hampton research, but I cannot find them there anymore. Does anyone know where these plates can be bought from? Schematic of the plate and its wells is here : http://www.google.com/patents/US5419278. Thanks, F
Re: [ccp4bb] cryoprotectant
Hi Reza, Check the following reference: Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme. Bujacz G, Wrzesniewska B, Bujacz A. Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):789-96. Cheers, Karolina W dniu 2014-12-01 10:59, Reza Khayat napisał(a): Hi, Has anyone used citrate as the sole cryoprotectant? If so, what concentration was needed? Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 www.khayatlab.org [1] Links: -- [1] http://www.khayatlab.org
[ccp4bb] Intensities and amplitudes
Dear crystallographers Is there any reason for using one data type over the other? Are there any errors associated with the French and Wilson I-to-F conversion step? Thanks. Mohamed
Re: [ccp4bb] CrystalClear tape rolls
I use 3 clear duck tape. Amazon has it in packages of 6 rolls. Seems to work OK, with maybe one well in a 2x96 well screen interacting with the adhesive. I think it's an isopropanol or dioxane condition. Roger Rowlett On Dec 1, 2014 5:32 AM, Mark J van Raaij mjvanra...@cnb.csic.es wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij
Re: [ccp4bb] CrystalClear tape rolls
Sent from my iPhone On Dec 1, 2014, at 14:22, Roger Rowlett rrowl...@colgate.edu wrote: I use 3 clear duck tape. Amazon has it in packages of 6 rolls. Seems to work OK, with maybe one well in a 2x96 well screen interacting with the adhesive. I think it's an isopropanol or dioxane condition. Roger Rowlett On Dec 1, 2014 5:32 AM, Mark J van Raaij mjvanra...@cnb.csic.es wrote: Dear All, Just wondering what the current situation on CrystalClear tray sealing tape is. Molecular Dimensions, Hampton, Jena seem to be selling mainly sheets or strips precut for plates - I guess related with the fact that the consumer box sealing tape changed specifications and now clouds over with certain reservoir solutions. See: https://www.mail-archive.com/ccp4bb@dl.ac.uk/msg00622.html We are stilll using rolls which came with plates we bought years back, but now only have two rolls left - so I am wondering if there is a more economic solution than buying sheets or strips. Greetings, Mark Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij
[ccp4bb] Postdoctoral Position - structure and mechanism of tRNA modification enzymes
Institution: Western University of Health Sciences - Pomona Campus Location: Pomona, CA Start Date: immediately Contact: Manal A. Swairjo, Ph.D. mswai...@westernu.edu We are looking for an outstanding and highly motivated individual to start immediately in a NIH-funded postdoctoral position to study structure and mechanism of transfer-RNA modification enzymes that constitute a new class of candidate antibacterial drug targets. The laboratory is part of a long-standing multi-institutional consortium focused on studying the biosynthesis and functions of the hypermodified nucleotides of transfer-RNA and understand their roles in translation. The project aims to elucidate the mechanistic and structural basis for the biosynthesis of a universally conserved hypermodified nucleotide of the anti-codon stem-loop of tRNA using a multi-disciplinary approach comprising X-ray crystallography, NMR spectroscopy, and a variety of biophysical and biochemical methods. The candidate will focus on cloning, overexpression, purification, biochemical characterization, crystallization, and X-ray crystal structure determination of multiple protein and protein-RNA complexes and interface with the PI and team of collaborators to complete functional and mechanistic interpretation of results toward development of this critical metabolic pathway as an antibacterial target. The candidate should have a Ph.D. degree (in biochemistry, biophysics, structural biology or related area) with a strong publication record. In addition, the candidate is required to be proficient in molecular biology, recombinant DNA methods, protein and RNA expression and purification, and troubleshooting and optimization of cloning and overexpression protocols of difficult and insoluble proteins. Prior experience in macromolecular X-ray crystallography is desired. Competitive salary and fringe benefits will be provided. To apply, please send a cover letter stating your scientific interests and goals, CV and contact information for 3 references to me at mswai...@westernu.edu. Western University of Health Sciences is a private, non-profit university and one of the largest graduate schools for the health professions in California, offering 21 academic programs in nine colleges, and operating two patient care centers. The main campus is located near historic downtown Pomona, the fifth largest city in Los Angeles County, California. Pomona is 27 miles east of Los Angeles, 9 miles from Ontario International Airport, and 45 miles from Los Angeles International Airport. Since 2012, WesternU has been named a Great College to Work For by The Chronicle of Higher Education. It is the policy of WesternU to provide equal employment opportunity to all employees and applicants for employment as required by law without regard to age, race, religion, sex, national origin, marital status, disability, mental or physical handicap, sexual orientation or any other basis protected by law. To this end, WesternU is committed to equal opportunity laws that ensure applicants and employees are treated equally. WesternU will strive not to unlawfully discriminate against any employee or applicant in employment decisions based on age, race, religion, sex, national origin, marital status, disability, mental and physical handicap, sexual orientation or any other basis protected by law. Additionally, WesternU is committed to ensuring that all employees enjoy a work environment that is free from all forms of unlawful harassment and intimidation. Manal A. Swairjo, Ph.D. Assistant Professor Graduate College of Biomedical Sciences Western University of Health Sciences 309 E. Second Street Pomona CA 91766-1854 Cell: 619 206 3864 Office: 909 469 8201 Lab: 909 469 8222 Fax: 909-469-5577 email: mswai...@westernu.edumailto:mswai...@westernu.edu website: http://www.westernu.edu/biomedical-sciences/biomedical-sciences-research/biomedical-sciences-swairjo-lab/
