Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

[luzuok]

You can also try products from Bio-rad.
http://www.bio-rad.com/featured/en/nickel-columns-nickel-resin.html


Best!



--

卢作焜
南开大学新生物站A202


Lu Zuokun, Ph.D. Candidate
College of Life Science, Nankai University

At 2017-02-18 04:31:08, "Kevin Jin"  wrote:

Sorry, the company was CloneTech, now it is Takara (not Takeda). You may talk 
with Dr. Gia Jokhadze, who is the director of protein chemistry department. He 
would give you more information.  You can find his profile on linkedin. He is 
very nice person. Probably, you can ask some free sample from him. 


The advantage of their clone: first, high capacity and better column 
performance(plate numbers). 


On Fri, Feb 17, 2017 at 9:33 AM, Kevin Jin  wrote:

Probably you can try His-Column from Clonetech (Takeda ?). They modified the 
column packing using nylon membrane, which offered better flow-through than 
that of traditional resin-gel column. 






On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger  
wrote:

Dear all,

we are happy users of all that GE offers around their FPLC system, but I am 
getting a little tired of feeling monopolized. Are any of you aware of either 
empty columns or prepacked columns (e.g. metal affinity or ion exchange resins) 
from other companies?

Thanks for your advice


Markus






 





--

Kevin Jin
 
Sharing knowledge each other is always very joyful..
 
Website: http://www.jinkai.org/
 

[ccp4bb] Bergen, Norway: 14 PhD and 3 post-doc positions open

[Petri Kursula]

Hi all,

The Faculty of Medicine and Dentistry in Bergen, Norway has 14 PhD student and 
3 post-doc positions available. Bergen is a beautiful, vibrant, modern 
Scandinavian city with a high standard of living, plenty of opportunities for 
outdoor activities, and good connections to the rest of Europe. 

For more details, see:

https://www.jobbnorge.no/en/available-jobs/job/134170/phd-positions-14-positions
 


and

https://www.jobbnorge.no/en/available-jobs/job/134181/postdoctoral-fellow-3-positions
 


Note: the candidates for all positions must, in addition to other relevant 
documents, provide a detailed research plan linked to a research group at the 
faculty, as well as a statement from the supervisor. Hence, prior contact, well 
in time, to a research group is an absolute pre-requisite.

The newly established, ambitious structural biology groups at the Faculty 
include e.g. projects on structural neurobiology 
(http://www.uib.no/en/rg/petrikursula 
) and malaria parasite cytoskeleton 
and motility  (http://www.uib.no/en/rg/inari 
). 

Interested qualified candidates are encouraged to contact one of the the 
corresponding group leaders with their CVs as soon as possible for 
pre-screening and possible application preparation, as the application deadline 
of March 12th is approaching fast.

Contact for structural neurobiology: Petri Kursula (petri.kurs...@uib.no 
)
Contact for the malaria project: Inari Kursula (inari.kurs...@uib.no 
)

Petri Kursula
--
Professor 
Department of Biomedicine
University of Bergen, Norway
http://www.uib.no/en/rg/petrikursula 

petri.kurs...@uib.no 
--
Group Leader, Adjunct Professor
Faculty of Biochemistry and Molecular Medicine
University of Oulu, Finland
--

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

[Kevin Jin]

Sorry, the company was CloneTech, now it is Takara (not Takeda). You may
talk with Dr. Gia Jokhadze, who is the director of protein chemistry
department. He would give you more information.  You can find his profile
on linkedin. He is very nice person. Probably, you can ask some free sample
from him.

The advantage of their clone: first, high capacity and better column
performance(plate numbers).

On Fri, Feb 17, 2017 at 9:33 AM, Kevin Jin  wrote:

> Probably you can try His-Column from Clonetech (Takeda ?). They modified
> the column packing using nylon membrane, which offered better flow-through
> than that of traditional resin-gel column.
>
>
>
> On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger <
> markus.seeli...@gmail.com> wrote:
>
>> Dear all,
>> we are happy users of all that GE offers around their FPLC system, but I
>> am getting a little tired of feeling monopolized. Are any of you aware of
>> either empty columns or prepacked columns (e.g. metal affinity or ion
>> exchange resins) from other companies?
>> Thanks for your advice
>>
>> Markus
>>
>
>
>
>
>



-- 
Kevin Jin

Sharing knowledge each other is always very joyful..

Website: http://www.jinkai.org/

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

[Kevin Jin]

Probably you can try His-Column from Clonetech (Takeda ?). They modified
the column packing using nylon membrane, which offered better flow-through
than that of traditional resin-gel column.



On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger 
wrote:

> Dear all,
> we are happy users of all that GE offers around their FPLC system, but I
> am getting a little tired of feeling monopolized. Are any of you aware of
> either empty columns or prepacked columns (e.g. metal affinity or ion
> exchange resins) from other companies?
> Thanks for your advice
>
> Markus
>

Re: [ccp4bb] Phaser MR pairwise percent packing criterion

[Randy Read]

Hi,

The old approach of counting the number of clashes was changed to the 
percentage of trace atoms (typically CA atoms) involved in clashes largely 
because, as people like Eleanor pointed out to us, the number of clashes grows 
with the size of the problem and solutions can be rejected when the fraction of 
the structure involved in clashes is still very small.  The default now is to 
accept up to 10% of the trace atoms being involved in clashes.  So increasing 
that to a larger number should have the same basic effect as increasing the 
number of allowed clashes did in the old version.

If you want to get sophisticated, there's even another new feature, where you 
can use the ENSEMBLE…TRACE command to define a TRACE molecule.  That will be 
carried along with the actual search model and used to check for clashes.  This 
might be useful if, for instance, you think a domain might change  conformation 
slightly so you don't want it in the search model, but you want to exclude 
solutions that would create clashes with that domain.

Best wishes,

Randy Read

> On 17 Feb 2017, at 08:28, Xiao Lei  wrote:
> 
> Dear CCP4bb members,
> 
> I found in the newer CCP4 Phaser MR version (I use CCP4 7.0.0 in Win7), there 
> is no "allow maximal clash..." option anymore. There are only two options 
> "pairwise percent packing" and "accept all solutions".  I used to increase 
> the number in the "allow maximal clash" (let's say from 100 to 200) to do MR 
> and this works fine in a couple of cases.
> 
> With the new Phaser version, I could not do this anymore, can I understand 
> that I just need to increase the pairwise percent packing cutoff (let's say 
> from 5% to 10%) to loose the tight packing criteria and allow more clashes in 
> finding solutions? 
> 
> 

--
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research  Tel: + 44 1223 336500
Wellcome Trust/MRC Building   Fax: + 44 1223 336827
Hills RoadE-mail: rj...@cam.ac.uk
Cambridge CB2 0XY, U.K.   www-structmed.cimr.cam.ac.uk

[ccp4bb] Phaser MR pairwise percent packing criterion

[Xiao Lei]

Dear CCP4bb members,

I found in the newer CCP4 Phaser MR version (I use CCP4 7.0.0 in Win7),
there is no "allow maximal clash..." option anymore. There are only two
options "pairwise percent packing" and "accept all solutions".  I used to
increase the number in the "allow maximal clash" (let's say from 100 to
200) to do MR and this works fine in a couple of cases.

With the new Phaser version, I could not do this anymore, can I understand
that I just need to increase the pairwise percent packing cutoff (let's say
from 5% to 10%) to loose the tight packing criteria and allow more clashes
in finding solutions?