[ccp4bb] postdoc on cryo-tomography of COPII at Birkbeck College

[Giulia Zanetti]

Dear all,

applications for the first of two postdoctoral positions in my lab are now open.

We use cryo-EM to understand COPII coat assembly and membrane remodelling. In 
particular, we employ cryo-tomography and subtomogram averaging to achieve 
detailed views of the coat assembled on membranes (see Hutchings et al., Nat 
Comm. 2018).
Our lab is part of the vibrant cryo-EM community at ISMB, Birkbeck College, 
where we have access to state-of-the-art facilities.


Please do get in touch with me if you would like to know more about this 
BBSRC-funded project. Closing date for applications is 31st October 2019.

https://cis7.bbk.ac.uk/vacancy/postdoctoral-researcher-401082.html 



Best,
Giulia

zanettilab.co .uk


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[ccp4bb] DLS/CCP4 data analysis workshop 2019

[David Waterman]

PhD students, postdocs and early career scientists,

Please consider applying for the sixth joint DLS/CCP4 workshop on MX data
collection and structure solution, to be held at Diamond Light Source, UK
from the 1st to the 8th December 2019.

This course offers the opportunity for you to work alongside leaders in the
field of MX on data from your own crystals. For more details please check
here:

http://www.ccp4.ac.uk/schools/DLS-2019/

The workshop has been very successful in previous years, as shown by
acknowledgements given in publications from attendees of the course:

http://www.ccp4.ac.uk/schools/DLS-school/index.php

The application period is now open and will* close on the 18th October*.
Applicants will be required to submit a CV/resume, describe their projects
and obtain a letter of support from a supervisor, so please do not wait
until the last minute to apply! Those with crystals and/or data will be
given priority.

Best wishes on behalf of the organisers,

David Waterman (CCP4)



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Re: [ccp4bb] no tNCS and Phaser manuals

[Airlie McCoy]

ps can you send the figure for all WITHOUT the less than 90%?

On Oct 1 2019, IRACEMA CABALLERO MUÑOZ wrote:


(Message body was not text: suppressed)




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[ccp4bb] no tNCS and Phaser manuals

[Herman . Schreuder]

Dear community,

I wanted to switch off the tNCS for a Phaser run, but could not find any Phaser 
manuals online, explaining how to do this.
So I have two questions:

  1.  How do I switch off tNCS in Phaser and
  2.  Are there any phaser manuals online? I tried hard to find them using 
Bing, but the websites were either not present, not available or could not be 
found. It may be that our firewall is also blocking certain sites although most 
sites are accessible.

Thank you very much for your help!
Herman






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Re: [ccp4bb] 2Fo-Fc maps in Coot vs. Pymol

[Chris Fage]

Dear Murpholino,

Yes, I think you're right. For now, directly exporting my maps from Coot is
the most efficient solution. The normalization commands will certainly be
helpful to me and other users in the future, though. Thanks!

Best,
Chris


Virus-free.
www.avg.com

<#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>

On Mon, Sep 30, 2019 at 9:02 PM Murpholino Peligro 
wrote:

> I think this is part of the problem.
> https://pymolwiki.org/index.php/Normalize_ccp4_maps
>
> El lun., 30 de sep. de 2019 a la(s) 10:09, Chris Fage (fage...@gmail.com)
> escribió:
>
>> Hi Paul,
>>
>> After exporting the maps from Coot, they are directly comparable in
>> Pymol. That's quite a convenient feature I was never aware of. Thanks!
>>
>> Yes, I was comparing the map from FFT in Coot and PyMOL.
>>
>> Best,
>> Chris
>>
>>
>> 
>>  Virus-free.
>> www.avg.com
>> 
>> <#m_-60911564517643226_m_-6662908832285936499_DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
>>
>> On Mon, Sep 30, 2019 at 3:04 PM Paul Emsley 
>> wrote:
>>
>>> On 30/09/2019 13:00, Chris Fage wrote:
>>> > Dear Paul, Herman, Robbie, and Santosh,
>>> >
>>> >
>>> > My version of Pymol doesn't support loading of mtz files. I think it's
>>> > only in the incentive version.
>>> >
>>> > Paul wrote: "No need to do this - just export the map (or the map
>>> > fragment)." I'm not sure how to do this without going through FFT!
>>>
>>>
>>> File -> Export Map...
>>>
>>>
>>> >
>>> > But when I generate a new set of maps with F1=FWT and PHI=PHWT and
>>> > load them into Pymol, they are still not comparable to those in Coot.
>>> > Again, the maps for ligands 1 and 2 in Pymol look about the same.
>>>
>>>
>>> Are you comparing the map from FFT in Coot and PyMOL?
>>>
>>>
>>> Paul.
>>>
>>> 
>>>
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>>>
>>
>> --
>>
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>>
>



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[ccp4bb] Call for MX beamtime proposals at SLS, approaching deadline

[Olieric Vincent (PSI)]

===
SYNCHROTRON BEAMTIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS
===

Proposal application deadline: October 15, 2019

Periods:
January 1, 2020 - June 30, 2020 (Normal proposals)
January 1, 2020 - December 31, 2021 (Long-term proposals)

Proposal submission:
http://www.psi.ch/sls/px-beamlines-call-for-proposals

Visit http://www.psi.ch/sls/pxi (X06SA-PXI) and http://www.psi.ch/sls/pxiii 
(X06DA-PXIII) for more information.

Travel support is available via CALIPSOplus (http://www.calipsoplus.eu/)


Best regards,

The MX group at SLS




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[ccp4bb] Group Leader position - Institute of Biotechnology, University of Helsinki

[Kajander, Tommi A]

Dear All,

I would like to draw your attention to the open group leader position at 
Institute of Biotechnology, University of Helsinki, Finland:

https://www.helsinki.fi/en/open-positions/a-group-leader-institute-of-biotechnology-hilife

Structural biology one of the main fields - if you feel you are a good 
candidate please do apply - in particularly internationally high level 
macromolecular
crystallography applications needed,  while the call is open to all fields of 
research in biosciences as specified in the call text. Selection is solely 
based on excellence.
Early career stage researchers are especially encouraged to apply, but please 
read the advertisement.

Salary is competitive and Institute of Biotechnology was ranked as one the 
flagships of research at UH in the recent evaluations.

From the advertisement text:

We offer an international, inspiring, and supportive research environment, 
along with excellent research facilities including in-house state-of-the art 
technologies in imaging, genomics, proteomics, CryoEM, crystallography, and 
NMR. The successful candidate will also enjoy access to all HiLIFE 
infrastructure platforms 
(https://www.helsinki.fi/en/helsinki-institute-of-life-science/hilife-res...).
 The salary is based on the salary system of the Finnish universities for 
teaching and research personnel, depending on the appointees’ qualifications, 
career stage and experience. The position also comes with an attractive 
negotiable 3-5 year startup package.

The University of Helsinki offers comprehensive services to its employees, 
including occupational health care, health insurance, sports facilities, and 
opportunities for professional development.

Finland is a member of the EU, has high quality free schooling (also in 
English), generous family benefits, and excellent healthcare.

Finland was recently ranked as the best country in the world for expat families 
and Helsinki in the world’s top ten most livable cities.

For more information about working at the University of Helsinki and living in 
Finland, please see 
https://www.helsinki.fi/en/university/working-at-the-university.


Best regards,
Tommi


Tommi Kajander, PhD
Principal investigator
Structural Biology Program
Institute of Biotechnology
Biocenter 3, Viikinkaari 1 (PO Box 65)
University of Helsinki
tommi.kajan...@helsinki.fi
+358 294158904







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