Re: [ccp4bb] Protocol for bacmid transformation in E.coli
Yes It requires electroporation and very careful handling of the bacmid. Other than that it is a fairly simple process. Here is an example reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067234/ Artem On Sat, Jul 18, 2020, 9:12 PM Digant Nayak wrote: > Dear all, > > Sorry for the off topic question, but i assure you that the long term > output of the project has structural biology application. I want to > transform a bacmid into E.coli cell and I was intrigued to find that there > is no protocol for this available on the internet (i would really > appreciate it if somebody proves me wrong and directs me to a link). Is it > similar to transforming a plasmid in E.coli? I would really like to hear > your experiences on this topic. > > Thanks, > Digant > > > > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Protocol for bacmid transformation in E.coli
Dear all, Sorry for the off topic question, but i assure you that the long term output of the project has structural biology application. I want to transform a bacmid into E.coli cell and I was intrigued to find that there is no protocol for this available on the internet (i would really appreciate it if somebody proves me wrong and directs me to a link). Is it similar to transforming a plasmid in E.coli? I would really like to hear your experiences on this topic. Thanks, Digant To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Accessing full list of programs in CCP4I2
I re-read this thread because I was confused by it, esp. the discussion on having to use the command line to run old programs! I assumed therefore that the old ccp4i gui had finally disappeared. Anyhow, I just installed 7.1 and it's still there, complete with the Program List dropdown! In case anyone else (who is yet to upgrade) was confused, there seems to be no need to panic, just yet!! Best wishes, Jon Cooper, E-mail: jon.b.coo...@protonmail.com To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Sad News
I'm writing to acknowledge the passing of Ward Smith during the weekend of 5 July. Ward got his PhD with Martha Ludwig at U. of Michigan, and then came to UCLA in 1977 to join Dave Eisenberg’s group as a postdoc. During the course of things, he met Cheryl Janson, a Paul Boyer postdoc, and they were married in 1980. During his time at UCLA Ward became expert in operation of the Xuong/Hamlin/Nielsen multi-wire system at UCSD and tutored the UCLA users in its use. In 1985 Ward and Cheryl left UCLA and went to Monsanto in St. Louis, where Ward worked as a structural biologist. In 1987 they went to Agouron Pharmaceuticals in San Diego. And then in 1995 went cross-country to SmithKline Beecham (which became Glaxo SmithKline, merging with GlaxoWellcome). Ward was very involved with getting IMCA set up as a functional facility for pharmaceuticals at Argonne as SmithKline's representative. This experience gave him significant credibility in synchrotron macromolecular crystallography, and in 2003 he joined the GM/CA-CAT beamlines at the APS to help Bob Fischetti and others construct that excellent facility. During this time Cheryl worked at Shamrock Structures. Ward moved to the NIH headquarters in 2007. There he took some responsibility for the Protein Structure Initiative, also playing an important role in supporting NIH synchrotron facilities. In 2010 he became the branch chief for the Structural Genomics and Proteomics Technology Branch in the Division of Cell Biology and Biophysics. He remained in that position through 2017. At the 2018 NIGMS re-organization Ward went to the Biophysics, Biomedical Technology, and Computational Biosciences division as the branch chief for the Biomedical Technology Branch. Ward helped oversee the big NIH-funded, $45 M construction of three major beamlines at NSLS-II, a project called ABBIX that ran 2011-2017. In 2017 he became program director for NIH support of structural biology beamlines at NSLS-II and other DOE synchrotrons. Many knew Ward for his always calm, reasoned demeanor; he was unflappable, resilient, and friendly. He was well read and devoted to his family. Robert M. Sweet E-Dress: sw...@bnl.gov Scientific Advisor, CBMS: The Center for BioMolecular Structure at NSLS-II Photon Sciences and Biology Dept Brookhaven Nat'l Lab. Upton, NY 11973 U.S.A. Phones: 631 344 3401 (Office) 631 338 7302 (Mobile) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
