[ccp4bb] reminder Postdoc CryoEM postion -deadline August 31
Dear all, A reminder that we currently have an open postdoc position in our Institute! Closing date is August 31st 2022. The Panne group focuses on signal processing on enhancers and how 3D genome organisation contributes to various aspects of genome function. For examples of recent work see Nature 578, 472–476 (2020); NSMB 27, 233–239 (2020); Nature 562, 538–544 (2018). We are located at the the Leicester Institute of Structural and Chemical Biology (LISCB) is a cross-disciplinary entity that brings together internationally renowned research in structural biology, single molecule approaches and chemical biology at the University of Leicester. The Institute provides access to world-class research facilities, including X-ray crystallography, biological NMR spectroscopy, single molecule microscopy and the state-of-the-art Midlands Regional Cryo-EM facility. About you Successful candidate should have a background in structural studies by single particle cryoEM. They must have a track record in conducting internationally competitive research. We are looking for a creative and ambitious person with good communication skills and keen to work on challenging projects. LISCB is one of five flagship research institutes recently established by the University of Leicester, UK. We have excellent access to state-of-the-art structural biology technologies including a Krios cryo-EM microscope equipped with K3/BioQuantum and in house GPU cluster for data processing. For further information and to apply please see the link below: https://jobs.le.ac.uk/vacancies/4527/research-associate-structural-biology-of-genome-regulation.html Please get in touch if you have questions concerning this role. All the best, Daniel Panne Department of Molecular and Cell Biology University of Leicester Lancaster Road, Leicester, LE1 7RH, UK T: +44 (0) 116 229 7408 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Quantifying electron density inside of a given volume
Dear Neno, when I asked the same question a few months ago, I ended up writing my own program. We used it for our study of coking in the zeolite ZSM5 (https://doi.org/10.1002/anie.202205413). It is not at all user friendly, and it reads in FCF files, rather than MTZ files. I can send you the source code nevertheless in case you are interested, or a linux binary. It is easy to create an FCF file from your MTZ file with e.g. pdb2ins and a quick run of SHELXL with 'CGLS 0'. Best, Tim On Wed, 10 Aug 2022 09:59:55 -0400 Neno Vuksanovic wrote: > Dear All, > > I would like to quantify electron density inside of positive Fo-Fc > blobs in active sites of multiple protomers in the map and compare > them. I am aware that I can interpolate maps and obtain density > values at coordinate points using either MapMan, Chimera or Coot, but > I would like to know if there is a tool that would let me designate a > sphere of a certain volume and calculate total electron density > inside it? > > Best Regards, > Neno > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ -- -- Tim Gruene Head of the Centre for X-ray Structure Analysis Faculty of Chemistry University of Vienna Phone: +43-1-4277-70202 GPG Key ID = A46BEE1A To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ pgp7oYTz8Cc4z.pgp Description: OpenPGP digital signature
