from:"Benini Stefano"

[ccp4bb] two year call for a research assistant

2022-02-04 Thread Benini Stefano

Dear All
We have a call for a two years (24 months) position for a postdoc/master with
experience in protein purification, characterization and structure
determination by X-ray crystallography for the project:

Discovering Apple Quality Markers for a Sustainable Conservation in Dynamic
Controlled Atmosphere (DAMaSCo)

The sustainable reduction of post-harvest losses can be achieved by regulating
apple metabolism during storage. A decrease of oxygen to concentrations ranging
from 2-3 to 1 % is used to extend the storage life of apples (Malus domestica)
in controlled atmosphere (CA). In dynamic CA (Dynamic CA, DCA), oxygen levels
are set at concentrations as low as 0.4 %. However, in DCA oxygen reduction
reaches the lowest level tolerated by the fruit with a high risk of severe
quality loss.
Aims of the project “DAMaSCo ” are:
• identification of quality markers (genes and metabolites) to
prevent decay of apples during storage in DCA. (university of Padua)
• characterization of the M. domestica enzymes differentially
expressed in low oxygen conditions. (university of Bolzano)
• elucidation of their reaction mechanisms and substrate specificity
(university of Bolzano).
The project “DAMaSCo” brings together in a multidisciplinary collaborative
effort Dr Stefano Benini and Sanja Baric (Free University of Bolzano, UNIBZ),
Prof. Benedetto Ruperti (University of Padua, UNIPD), Prof. Francesco Musiani
(University of Bologna, UNIBO), Professor Michele Cianci (Università
Politecnica delle Marche)

The salary is €24000/year (48000€).
For the call and to apply online please visit:

https://www.unibz.it/it/home/position-calls/positions-for-academic-staff/5654-biochimica-prof-baric?group=18

Best regards

S.
Stefano Benini, Ph.D. Assistant Professor
Stefano Benini - (unibz.it)
https://sbenini.people.unibz.it

https://sbenini.people.unibz.it/bioorganic-chemistry-bio-crystallography-laboratory/

“And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good.” Saul Bellow

“articolo 21 della Costituzione Italiana: Tutti hanno diritto di manifestare
liberamente il proprio pensiero con la parola, lo scritto e ogni altro mezzo di
diffusione.”
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology, Libera Università di Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14): +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X
Scopus: https://www.scopus.com/authid/detail.uri?authorId=7004187955

"ogni giorno in più è un giorno in meno…" copyright Stefano Benini!
“dignità e libertà non hanno prezzo”
“I don’t like anything that’s fake and I hate pretenders!” Stefano Benini

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[ccp4bb] REMINDER ! 24 months position for a postdoc c/o Free University of Bolzano deadline 19/11/2021 REMINDER!

2021-11-06 Thread Benini Stefano

Dear All,

We have a call for a two years (24 months) position for a postdoc with
experience in protein purification, characterization and structure
determination by X-ray crystallography for the project:

Discovering Apple Quality Markers for a Sustainable Conservation in Dynamic
Controlled Atmosphere (DAMaSCo)

The work will involve:

* Purification and characterization of the M. domestica enzymes
differentially expressed in low oxygen conditions. (c/o university of Bolzano)

* elucidation of their reaction mechanisms and substrate specificity (c/o
university of Bolzano).

The salary is €24000/year.
For the call and to apply online please visit:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/5485-chimica-organica-dr-benini?group=18

Enquiries about the project could be addressed to me

More details below
Best regards
S.

Discovering Apple Quality Markers for a Sustainable Conservation in Dynamic
Controlled Atmosphere (DAMaSCo)

* identification of quality markers (genes and metabolites) to prevent
decay of apples during storage in DCA. (university of Padua)
* characterization of the M. domestica enzymes differentially expressed in
low oxygen conditions. (university of Bolzano)

* elucidation of their reaction mechanisms and substrate specificity
(university of Bolzano).
The project “DAMaSCo” brings together in a multidisciplinary collaborative
effort
Dr Stefano Benini (Free University of Bozen-Bolzano, UNIBZ), Prof. Benedetto
Ruperti (University of Padua, UNIPD), Prof. Francesco Musiani (University of
Bologna, UNIBO)

The salary is €24000/year (48000€).
For the call and to apply online please visit:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/5485-chimica-organica-dr-benini?group=18

Best regards

Stefano Benini, Ph.D. Assistant Professor

https://sbenini.people.unibz.it/bioorganic-chemistry-bio-crystallography-laboratory/

“And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good.” Saul Bellow

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[ccp4bb] two years (24 months) position for a postdoc c/o Free University of Bolzano

2021-10-19 Thread Benini Stefano

Dear All job seekers et al.,

We have a call for a two years (24 months) position for a postdoc with 
experience in protein purification, characterization and structure 
determination by X-ray crystallography for the project:

Discovering Apple Quality Markers for a Sustainable Conservation in Dynamic 
Controlled Atmosphere (DAMaSCo)

The sustainable reduction of post-harvest losses can be achieved by regulating 
apple metabolism during storage. A decrease of oxygen to concentrations ranging 
from 2-3 to 1 % is used to extend the storage life of apples (Malus domestica) 
in controlled atmosphere (CA). In dynamic CA (Dynamic CA, DCA), oxygen levels 
are set at concentrations as low as 0.4 %. However, in DCA oxygen reduction 
reaches the lowest level tolerated by the fruit with a high risk of severe 
quality loss.
Aims of the project “DAMaSCo ” are:

  *   identification of quality markers (genes and metabolites) to prevent 
decay of apples during storage in DCA. (university of Padua)
  *   characterization of the M. domestica enzymes differentially expressed in 
low oxygen conditions. (university of Bolzano)

  *   elucidation of their reaction mechanisms and substrate specificity 
(university of Bolzano).
The project “DAMaSCo” brings together in a multidisciplinary collaborative 
effort
Dr Stefano Benini (Free University of Bozen-Bolzano, UNIBZ), Prof. Benedetto 
Ruperti (University of Padua, UNIPD), Prof. Francesco Musiani (University of 
Bologna, UNIBO)

The salary is €24000/year (48000€).
For the call and to apply online please visit:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/5485-chimica-organica-dr-benini?group=18

Best regards

Stefano Benini, Ph.D. Assistant Professor

https://sbenini.people.unibz.it/bioorganic-chemistry-bio-crystallography-laboratory/

“And money wasn't what I had in mind. Oh God, no, what I wanted was to do good. 
I was dying to do something good.” Saul Bellow

“articolo 21 della Costituzione Italiana:  Tutti hanno diritto di manifestare 
liberamente il proprio pensiero con la parola, lo scritto e ogni altro mezzo di 
diffusione.”
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology, Libera Università di Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X
Scopus: https://www.scopus.com/authid/detail.uri?authorId=7004187955





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[ccp4bb] REMINDER: two years postdoctoral fellowship, deadline for sending the application 22/01/2020

2020-01-03 Thread Benini Stefano

Dear All,

We have a call for a two years postdoc (24k€ a year) for candidates holding a
PhD and with experience in protein purification (experience in cloning is
desirable but not strictly required) to carry out:

Protein purification and crystallization, structural and functional
characterization of enzymes,.

Within the project: “SupErA - Siderophore mediated iron uptake in Erwinia
amylovora and Aspergillus fumigatus. Towards new strategies in plant and human
health”
you will be involved in the characterization of the biosynthetic enzymes from
the human pathogen A. fumigatus and be part of a collaborative effort between
the University of Bolzano, the Medical University of Innsbruck (professor
Hubertus Haas) and the Centre for Integrated Biology, University of Trento
(professor Sheref Mansy) to develop new molecules to be used in
therapy/diagnostics of both the human and the plant pathogen.

interested candidates can contact me for more information on the project and
for informal enquiries
to apply please follow the link:
https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/4468-chimica-organica-dr-benini?group=18

best regards
Stefano

P.S. Bolzano is located in the Dolomites area offering many hiking, climbing
and skiing opportunities

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products 2020"
A special issue of International Journal of Molecular
Sciences (IF 4.183) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes_2020
deadline for submission 30/06/2020

[https://s3.amazonaws.com/zapnito/uploads/f9805f649da7ceb38a2059ab1ce4f9bc/SciReports_EBM_Branded_Sig_v2.jpg]

https://sbenini.people.unibz.it/
“And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good.” Saul Bellow

“I don’t like anything that’s fake and I hate pretenders!” Stefano Benini

"ogni giorno in più è un giorno in meno…"

articolo 21 della costituzione: Tutti hanno diritto di manifestare liberamente
il proprio pensiero con la parola, lo scritto e ogni altro mezzo di diffusione..

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[ccp4bb] two years postdoctoral fellowship, deadline for sending the application 22/01/2020

2019-12-20 Thread Benini Stefano

Dear All,

Protein purification and crystallization, structural and functional
characterization of enzymes,.

best regards
Stefano

P.S. Bolzano is located in the Dolomites area offering many hiking, climbing
and skiing opportunities

[https://s3.amazonaws.com/zapnito/uploads/f9805f649da7ceb38a2059ab1ce4f9bc/SciReports_EBM_Branded_Sig_v2.jpg]

https://sbenini.people.unibz.it/
“And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good.” Saul Bellow

“I don’t like anything that’s fake and I hate pretenders!” Stefano Benini

"ogni giorno in più è un giorno in meno…"

articolo 21 della costituzione: Tutti hanno diritto di manifestare liberamente
il proprio pensiero con la parola, lo scritto e ogni altro mezzo di diffusione..

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] (off-topic) fundraising campaign - EMERGENCY VENICE

2019-11-19 Thread Benini Stefano

Dear All,

On behalf of Elisa Moretti (in Cc.) I share this fundraising initiative for
Venice that was recently damaged by flooding:

Dear all,

I am sharing with you the news about the initiatives launched by Ca' Foscari
University of Venice (Italy) in support of Venice and its citizens, hit hard by
the adverse weather conditions of the past few days.

Ca' Foscari has had its own damage in some of its sites, but these days it is
the whole city which is at its knees, with homes, businesses, libraries,
bookshops severely damaged, monuments soaked in salty waters, boarding stations
destroyed, boats sunk. . .

We have always been in Venice with our community, and on this emergency we
thought it is important to be proactive in supporting the city and its
residents.
We have thus decided to launch a fundraising campaign - EMERGENCY VENICE -
through our fundraising platform. It takes just a few minutes to donate, at
the following link:
https://supportacafoscari.unive.it/emergenzavenezia/~my-donation.
If you deem it appropriate, please contribute to the campaign and help us share
it with your contacts.

Thanks to all for what you will be able to do, even a small gesture means a lot!

Kindest regards,
Elisa Moretti

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 4.183) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes

[https://s3.amazonaws.com/zapnito/uploads/f9805f649da7ceb38a2059ab1ce4f9bc/SciReports_EBM_Branded_Sig_v2.jpg]

https://sbenini.people.unibz.it/
"And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good." Saul Bellow

"I don't like anything that's fake and I hate pretenders!" Stefano Benini

"articolo 21 della Costituzione Italiana: Tutti hanno diritto di manifestare
liberamente il proprio pensiero con la parola, lo scritto e ogni altro mezzo di
diffusione."
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology, Libera Università di Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14): +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X

"ogni giorno in più è un giorno in meno..."

articolo 21 della costituzione: Tutti hanno diritto di manifestare liberamente
il proprio pensiero con la parola, lo scritto e ogni altro mezzo di diffusione..

To unsubscribe from the CCP4BB list, click the following link:
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[ccp4bb] 3 years research assistant position for masters or junior postdocs

2019-08-21 Thread Benini Stefano

Dear All,

We have no internal candidates for the following position suitable either for a
researcher with a MSc or a junior postdoc with experience in protein
purification and possibly protein crystallization and crystallography.

The project:
Siderophore mediated iron uptake in Erwinia amylovora and Aspergillus
fumigatus. Towards new strategies in plant and human health. (SupErA)
The project is in collaboration with Professor Hubertus Haas, Medical
University Innsbruck, and Professor Sheref Mansy, University of Trento.

The project aims at the structural and functional characterization of proteins
and enzymes involved in iron uptake in both the bacterial and fungal systems.

Links with information on how to apply are at the following links
https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/4281-chimica-organica-dr-benini?group=18

call:
https://webservices.scientificnet.org/rest/uisdata/api/v1/tenders/attachments/43445

forms to apply:
https://webservices.scientificnet.org/rest/uisdata/api/v1/tenders/attachments/43446

Bolzano is located in the north of Italy at the border with Austria (2 hours
train from Innsbruck Austria, 30' from Trento, Italy)

for informal enquiries (and scientific questions!) do not hesitate to contact me

some background information on one of the system to study could be found in
this publication:
https://doi.org/10.1016/j.jsb.2018.02.002

deadline for application is 09/09/2019

have a nice day

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 4.183) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
Deadline for manuscript submissions: 31 October 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes

https://sbenini.people.unibz.it/
[https://s3.amazonaws.com/zapnito/uploads/f9805f649da7ceb38a2059ab1ce4f9bc/SciReports_EBM_Branded_Sig_v2.jpg]
https://www.unibz.it/en/faculties/sciencetechnology/academic-staff/person/27433-stefano-benini

"And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good." Saul Bellow

"I don't like anything that's fake and I hate pretenders!" Stefano Benini
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
"Free" University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14): +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X

"ogni giorno in più è un giorno in meno..."

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 4.183) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
Deadline for manuscript submissions: 31 October 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes

"And money wasn't what I had in mind. Oh God, no, what I wanted was to do good.
I was dying to do something good." Saul Bellow

"I don't like

[ccp4bb] 3 years Research assistant position deadline approaching 04/06/2019

2019-05-27 Thread Benini Stefano

Dear job seekers,

A 3 years Research assistant position (1+1+1) is available at the Free
University of Bolzano to work in my group on the Euregio IPN95 project:

"Siderophore mediated iron uptake in Erwinia amylovora and Aspergillus
fumigatus. Towards new strategies in plant and human health"

The project is in collaboration with Professor Hubertus Haas, Medical
University Innsbruck, and Professor Sheref Mansy, University of Trento.

To apply for the position you need to have at least expertise in gene cloning,
protein expression and protein purification

To apply follow the link:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/4094-chimica-organica-dr-benini?group=18

for informal enquiries (and scientific questions!) do not hesitate to contact me

some background information on one of the system to study could be found in
this publication:
https://doi.org/10.1016/j.jsb.2018.02.002

deadline for application is 04/06/2019

have a nice day

Stefano

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 3.69) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
Deadline for manuscript submissions: 31 July 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes
[Banner H_smaller]

https://sbenini.people.unibz.it/

"I don't like anything that's fake and I hate pretenders!"
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14): +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X

"ogni giorno in più è un giorno in meno..."

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https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] PhD position available at Free University of Bolzano, Italy

2019-05-09 Thread Benini Stefano

Dear All,

A PhD position fully funded for three years by the Free University of Bolzano
is available to work in my group.

The work involves gene cloning, protein expression, purification and
crystallization to solve the structure and characterize biochemically the
enzymes of interest.

The successful candidate will choose his/her PhD project between these 2:

1. Biomolecular characterization of the amylovoran biosynthetic pathway in the
plant pathogen Erwinia amylovora

2. Siderophore mediated iron uptake in the plant pathogen Erwinia amylovora and
in Aspergillus fumigatus

We have strong connections with international scientific institutions where
students spend their period of additional training

Information on how to apply could be found below:

https://www.unibz.it/assets/Documents/Applicants/PhD-35-Cycle/2019-05-07-PhD-MEA-2019-20-35-cycle-unibz-EN.pdf

https://www.unibz.it/assets/Documents/Applicants/PhD-35-Cycle/2019-05-07-PhD-Call-2019-20-35-cycle-unibz-EN.pdf

they are both on this page:

https://www.unibz.it/en/faculties/sciencetechnology/phd-mountain-environment-agriculture/

Bolzano is located in the north of Italy in the Dolomites area. Outdoor
activities include hiking, climbing, skiing
Not far from Trento, Garda Lake and Verona

Best regards

Stefano Benini, Ph.D. Assistant Professor

https://sbenini.people.unibz.it/

Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 3.69) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
Deadline for manuscript submissions: 31 July 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes
[Banner H_smaller]

"ogni giorno in più è un giorno in meno..."

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[ccp4bb] 3 years Research assistant position at the Free University of Bolzano (Italy)

2019-05-03 Thread Benini Stefano

Dear job seekers,

A 3 years Research assistant position (1+1+1) is available at the Free
University of Bolzano to work in my group on the Euregio IPN95 project:

"Siderophore mediated iron uptake in Erwinia amylovora and Aspergillus
fumigatus. Towards new strategies in plant and human health"

The project is in collaboration with Professor Hubertus Haas, Medical
University Innsbruck, and Professor Sheref Mansy, University of Trento.

To apply for the position follow the link:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/4094-chimica-organica-dr-benini?group=18

for informal enquiries (and scientific questions!) do not hesitate to contact me

some background information on one of the system to study could be found in
this publication:
https://doi.org/10.1016/j.jsb.2018.02.002

deadline for application is 04/06/2019

have a nice day

Stefano

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction
Products"
A special issue of International Journal of Molecular
Sciences (IF 3.69) (ISSN 1422-0067). This
special issue belongs to the section "Molecular
Biophysics".
Deadline for manuscript submissions: 31 July 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes
[Banner H_smaller]

https://sbenini.people.unibz.it/

"ogni giorno in più è un giorno in meno..."

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] PhD call at the Free University of Bolzano deadline July 9th 2018

2018-06-06 Thread Benini Stefano

Dear All,
A multidisciplinary PhD call is open at the Free University of Bolzano,

In the Faculty of Science and Technology within the Mountain Environment and 
Agriculture PhD program

Selected candidates could choose among the following line of research in my 
group:

Siderophore mediated Iron uptake in Erwinia amylovora

Exopolysaccharide biosynthetic pathway in Erwinia amylovora

Pathogenicity associated proteins in Erwinia amylovora

Dihydrochalcones biosynthesis in Malus domestica

Your PhD research work will involve cloning of the genes of interest followed 
by protein expression and purification followed by structural and functional 
characterization of proteins and enzymes via X-ray crystallography and other 
techniques in collaboration with research organization such as John Innes 
Centre, DIAMOND, ELETTRA, Fondazione Edmund Mach, Medical University Innsbruck, 
etc.,

The link to the PhD program page and call:

https://www.unibz.it/en/applicants/phd-programmes/

link to Mountain Environment and Agriculture PhD program:
https://www.unibz.it/en/faculties/sciencetechnology/phd-mountain-environment-agriculture/

to apply online (compulsory) please register via:

https://unibz.gomovein.com/locallogin/5a957c2b8ba3286cdb7dd142/eng


Applicants will be ranked by an independent selection panel

Deadline for completing the application: July 9th 2018 at 1 a.m.

Bolzano is located in the North of Italy in the heart of Dolomites not far from 
historical cities like Verona, Venice etc., moreover it's only about 30-45 
minute drive from sky resorts (e.g., Obereggen, Carezza, etc.,). With a cable 
car you can reach 1200 m in 10 minutes from city centre and go hiking in the 
woods...
http://www.bolzano-bozen.it/en/bolzano.htm


for informal enquiries please contact me... @
stefano.ben...@unibz.it

Best regards
S.

Stefano Benini, Ph.D. Assistant Professor
https://sbenini.people.unibz.it/

"I don't like anything that's fake and I hate pretenders!"
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X

"ogni giorno in più è un giorno in meno..."

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[ccp4bb] PhD call at the Free University of Bolzano, Faculty of Science and Technology

2015-05-26 Thread Benini Stefano

Dear All,

I would like to bring to your attention the public call for PhDs at the Faculty 
of Science and Technology  of the Free University of Bolzano.

Two projects on the structural and functional characterization of proteins 
involved in the pathogenicity of Erwinia amylovora, the agent responsible of 
Fire Blight in apple and pear, are available in my group for successful 
applicants:



Biomolecular and biophysical characterization of the proteins involved in iron 
uptake in Erwinia amylovora







Molecular characterization of the biosynthetic pathway of amylovoran, a 
pathogenicity factor in Erwinia amylovora






The PhD program official language is English

Links to the official application procedure are below:


http://www.unibz.it/SiteCollectionDocuments/Research%20Office/PhD/BANDI/Scheda%20descrittiva%20MEA_en_31%C2%B0ciclo.pdf

and

http://www.unibz.it/SiteCollectionDocuments/Research%20Office/PhD/BANDI/2015_16_Ausschreibung%20PhD_en%2031%20Zyklus_def.pdf



Best regards


Dr Stefano Benini, Ph.D.
Assistant Professor

Laboratory homepage:

http://pro.unibz.it/staff2/sbenini/B2Cl.htm

Personal homepage
http://pro.unibz.it/staff2/sbenini/

I don't like anything that's fake and I hate pretenders!

*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009

ogni giorno in più è un giorno in meno...

[ccp4bb] off-topic: protein losing FAD during purification

2014-03-13 Thread Benini Stefano (P)

Dear All (those dealing with wetlab stuff..),

While purifying a FAD containing protein we lose part of the FAD (on the gel 
filtration we clearly see two bands corresponding to holoprotein and free FAD).

We obtain crystals but diffracting to only about 4 A despite their beautiful 
look. Our hypothesis is that the crystals contain a population of molecules 
with and without FAD (?).

The questions are:

1) how to keep FAD bound to the protein during purification and crystallization?

2) how to completely remove FAD from the protein? 

Thank you very much for any help provided!

Best regards

Stefano (part-time wetlab person)


Dr Stefano Benini, Ph.D.
Assistant Professor

First International workshop: Molecular Basis of Fire Blight, Bolzano 
15.10.2014

Laboratory homepage:
http://pro.unibz.it/staff2/sbenini/B2Cl.htm

Personal homepage
http://pro.unibz.it/staff2/sbenini/

I don't like anything that's fake and I hate pretenders!

*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009

ogni giorno in più è un giorno in meno.

Re: [ccp4bb] video of crystal pick-up through microscope?

2013-09-16 Thread Benini Stefano (P)

And when handling liquid nitrogen don't forget to get protections against frost 
bite (goggles, cryogloves)

S.

-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Swastik 
Phulera
Sent: Friday, 13 September, 2013 15:08
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] video of crystal pick-up through microscope?

Hi,
May be this would help
https://www.youtube.com/watch?v=QcsaWowulDM

On Fri, Sep 13, 2013 at 2:51 PM, Frank von Delft frank.vonde...@sgc.ox.ac.uk 
wrote:
 Hi all - does anybody know where I can find a video of a crystal being 
 harvested, but taken through a microscope?

 The Google has not been helpful, but I'm obviouly incompetent with 
 these new-fangled things.

 Thanks!
 phx



--
--
स्वस्तिक फुलेरा
वरिष्ठ अनुसंधान कर्ता
संरचनात्मक जीवविज्ञान प्रयोगशाला
डी. एन. ए. फिंगरप्रिंटिंग एवं निदान केंद्र हैदराबाद, ५००, ००१

और

राष्ट्रीय कोशिका विज्ञान केंद्र
पुणे विश्वविद्यालय परिसर,
गणेशखिंड
पुना,  ४११,००७

[ccp4bb] PhD program at the Free University of Bolzano (Italy)

2013-08-08 Thread Benini Stefano (P)

Dear All,

a new call for applicants for a PhD fellowship at the Free University of 
Bolzano is now out. Please follow the link

http://www.unibz.it/en/public/research/phd/Documents/bando_dottorati_29esimo_ciclo_en.pdf


A suitable candidate will be selected for a position in my group at the

Faculty of Science and Technology
PhD programme in MOUNTAIN ENVIRONMENTS AND AGRICULTURE
Website:
http://www.unibz.it/en/sciencetechnology/progs/phd/phdmountainenvironment/default.html


The following two projects are available for the selected candidate


1)Biomolecular characterization of proteins from Erwinia amylovora's 
amylovoran biosynthetic pathway


2)Biomolecular characterization of proteins from Erwinia amylovora's 
bacteriophages. How to fight fire blight using its natural killer.

candidate interested are welcome to contact me for information bust MUST apply 
following the rules and links as specified in the call


other links

http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html

pre-enrole here:

https://aws.unibz.it/exup/en/Account/Logon?ReturnUrl=%2fexup%2fen%2fHome%2fIndex



Please note that Bolzano is located near the UNESCO site of the Dolomites with 
some of the best hiking paths and slopes for skiing.

http://whc.unesco.org/en/list/1237/

http://www.obereggen.com/en/winter_activities/


with my best regards


Dr Stefano Benini, Ph.D.
Assistant Professor

http://pro.unibz.it/staff2/sbenini/

I don't like anything that's fake and I hate pretenders!  Sir Arthur 
Thispairof

*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009

[ccp4bb] Reminder: PhD position in structural biology at the Free University of Bolzano

2012-07-23 Thread Benini Stefano (P)

Dear All (possible candidates!),


A 3 year PhD fellowship is available to a suitable candidate for one of the 
following projects involving molecular biology and structural biology 
techniques:

Molecular characterization of the bio synthetic pathway of amylovoran, a 
pathogenicity factor in Erwinia amylovora

Biomolecular study of iron uptake by desferrioxamine in the plant pathogen 
Erwinia amylovora

the work will involve cloning of genes, protein expression, purification and 
crystallization and structure determination by X-ray crystallography. Proteins 
will be characterized using in solution techniques including NMR available 
through collaboration with other laboratories.


To apply to the public competition please visit the link:


http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html



there you will find the link to the official call document:


http://www.unibz.it/SiteCollectionDocuments/2012_PhD_Ausschreibung_en.pdf

as well as the link to the pre-enrolment page:


https://aws.unibz.it/exup/

The applications must be sent by 31 August 2012.
It is recommended that applications are completed and sent as soon as possible

If you wish to apply and want more information on the project and for informal 
enquiries please contact: stefano.ben...@unibz.it
 
For information on how to apply please contact our secretary: 
stefania.falc...@unibz.it

Best regards

Stefano Benini, Ph.D.
Assistant Professor

http://pro.unibz.it/staff2/sbenini/

*
Laboratory of Bioorganic Chemistry and Crystallography Faculty of Science and 
Technology Free University of Bolzano Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.012): +39 0471 017901
Fax: +39 0471 017009

Re: [ccp4bb] Distinguish NH4 from Na?

2011-11-17 Thread Benini Stefano (P)

Dear Jacob, 

You may find useful this web site by Marjorie Harding about metal coordination 
sites in proteins
http://tanna.bch.ed.ac.uk/

best regards


Stefano Benini, Ph.D.
Assistant Professor

http://pro.unibz.it/staff2/sbenini/


Bio-organic Chemistry Laboratory
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.012): +39 0471 017901
Fax: +39 0471 017009
***


-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob 
Keller
Sent: Wednesday, 16 November, 2011 19:21
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Distinguish NH4 from Na?

Dear Crystallographers,

I have crystals containing 666mM NH4 and 540mM Na, and there appears
to be a water which is only about 2.2 Ang from some polar atoms. It
is currently reasonably happy as a Na, but is there any reasonable way
to decide which cation is there?

JPK

-- 
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
email: j-kell...@northwestern.edu
***

[ccp4bb] PhD position in structural biology at the Free University of Bolzano

2011-08-12 Thread Benini Stefano (P)

Dear All,

On behalf of the Faculty of Science and Technology 
(http://www.unibz.it/en/sciencetechnology/welcome/default.html)
of the Free University of Bolzano 
(http://www.unibz.it/en/public/university/default.html)

I am glad to advertise a position for a PhD on the structural biology of 
proteins from plants' bacterial pathogens within our multidisciplinary PhD 
program in Management of Mountain Environment.
Information on the PhD program as well as the forms required to apply 
(applications should be received by October 14th ) can be found at the 
following links:

http://www.unibz.it/en/sciencetechnology/progs/phd/phdmountainenvironment/default.html


http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html


With my best regards

Stefano Benini


Stefano Benini, Ph.D.
Assistant Professor

http://pro.unibz.it/staff2/sbenini/


Bio-organic Chemistry Laboratory
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.012): +39 0471 017901
Fax: +39 0471 017009
***

[ccp4bb] Enterokinase cleavage of His-tagged proteins

2011-08-02 Thread Benini Stefano (P)

Dear All,

 

I decided to use a LIC vector with enterokinase cleavage site for His tag 
removal. Of course I did not check the length of the linker (about 45 aa) nor 
if the enterokinase cleavage site would be the best choice

 

Do you know where I could purchase a decent quality with reasonable price 
enterokinase to cleave mgs of his-tagged proteins for crystallization?

 

Thank you very much and best regards

 

Stefano

 

 

 

Stefano Benini, Ph.D.

Assistant Professor

 

http://pro.unibz.it/staff2/sbenini/ http://pro.unibz.it/staff2/sbenini/ 

 



Bio-organic Chemistry Laboratory

Faculty of Science and Technology

Free University of Bolzano

Piazza Università, 5

39100 Bolzano, Italy

Office (room K2.14):  +39 0471 017128

Laboratory (room E.012): +39 0471 017901

Fax: +39 0471 017009

***

[ccp4bb] which Linux workstation for crystallography to buy?

2010-10-12 Thread Benini Stefano (P)

Dear All,

 

I need to buy a Linux workstation to run crystallographic software and graphics 
like ccp4, mosflm, coot., etc.,

Could you please suggest me a good combination of hardware and  which linux 
operating system to install (ubuntu?)? I can spend about 1500€

Technology evolves so fast that I really want to be up to date not to be 
already late!

 

Thank you very much in advance 

 

Stefano

 

 

Stefano Benini, Ph.D.

Assistant Professor

 

http://pro.unibz.it/staff2/sbenini/

 



Bio-organic Chemistry Laboratory

Faculty of Science and Technology

Free University of Bolzano

Piazza Università, 5

39100 Bolzano, Italy

Office (room K2.11):  +39 0471 017128

Laboratory (room E.012): +39 0471 017901

Fax: +39 0471 017009

***

[ccp4bb] Off topic: multidisciplinary international PhD program in Bolzano, Italy

2010-08-16 Thread Benini Stefano (P)

Dear All,

 

on behalf of the Faculty of Science and Technology of the Free University of 
Bolzano, 

 

I would like to announce our new multidisciplinary international PhD program 

 

The PhD official language is English. 

The research projects cover several scientific fields:

ecology, hydrology, fruit science, agricultural chemistry, biology, structural 
biology, microbiology, etc., 

 

More information are available at the following links:

 

PhD Programme in Management of Mountain Environment:

 

http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/default.html
 
http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/default.html
 

 

topics of PhD Thesis:

 

http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/thesis/default.html
 
http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/thesis/default.html
 

 

study program:

http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/studyplan/default.html
 
http://www.unibz.it/en/sciencetechnology/progs/phdmountainenvironment/studyplan/default.html
 

 

application forms:

http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html 
http://www.unibz.it/en/public/research/phd/prospectivePhdstudents.html 

  

The Free University of Bolzano: 

 

http://www.unibz.it/en/public/university/default.html

 

 

Bolzano is located in the north of Italy not far from the best slopes for 
alpine ski, hiking and climbing and the Dolomites (UNESCO heritage site 
http://whc.unesco.org/en/list/1237/). 

 

 

please do not reply to this email but if you are interested please refer to the 
links provided

or contact: science.technol...@unibz.it mailto:science.technol...@unibz.it 

 

Faculty of Science and Technology 

 piazza Università, 5

39100 Bolzano Italy 

T: +39 0471 017000

F: +39 0471 017009

 

With my best regards

 

Stefano Benini, Ph.D.

Assistant Professor

 

http://pro.unibz.it/staff2/sbenini/ http://pro.unibz.it/staff2/sbenini/ 

 



Bio-organic Chemistry Laboratory

Faculty of Science and Technology

Free University of Bolzano-Bozen

Piazza Università, 1

39100 Bolzano, Italy

***

Re: [ccp4bb] Dry structures

2007-08-16 Thread Benini, Stefano

Dear Elisabetta,


from the statistics you attach it seems that your low resolution extends only 
to 6.72 A

My guess is that if you could include as much low resolution  as possible (e.g. 
up to 20A or more)it will help a lot in getting better maps etc.,

I hope this is the answer you are looking for!!
please let me know what happens next!

ciao ciao

Stefano

***
Stefano Benini PhD
Structural Biology - DECS
Mereside 50S38
Alderley Park
Phone:+44-1625-518293 (ext.: 28293)
http://structuralbiology-ap.rd.astrazeneca.net/people/Stefano-Benini/Stefano-Benini.html
**


-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of
Sabini, Elisabetta
Sent: 15 August 2007 18:35
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Dry structures


Dear all,

what does it mean when a structure doesn't have many water molecules?

I have a 2.3A data set, 2 molecules in the AU (260 residues each), space
group C2221 - I haven't finished my water search but I don't seem to have
more than 20-30 obvious/good water molecules. The Rfac/Rfree are 22/30%,
the protein is modeled and the ligands are in.


I collected the data at APS SERCAT ID-22. Did I have a dry crystal (!!) or
did the beamline dried it up?!

How many water molecules should I expect at this resolution?

Also, what does it mean when a strong peak in the Fobs-Fc doesn't have the
corresponding 2Fobs-Fc map covering it even at very low sigma?

Thank you!

Eli :o)

PS: I have attached the statistics for the data set from XDS

-- 
Elisabetta Sabini, Ph.D.
Research Assistant Professor
University of Illinois at Chicago
Department of Biochemistry and Molecular Genetics
Molecular Biology Research Building, Rm. 1108
900 South Ashland Avenue
Chicago, IL 60607
U.S.A.

Tel: (312) 996-6299
Fax: (312) 355-4535
E-mail: [EMAIL PROTECTED]

Re: [ccp4bb] Help with reducing crystal mosaicity

2007-07-11 Thread Benini, Stefano

Dear Mary,

the problem encountered with cryoprotectans is the change in the solution 
surrounding your crystal as they may not be present in your crystallisation 
conditions or you need to increase their concentration to make them act as 
cryoprotectant.
So I don't thik is the cryoprotectant itself as it is you mother liquor..

test it in a capillary so you could rule out that freezing is the problem

of course you don't want to find new crystallisation conditions when you tried 
everything and I know what it means!!

I would try to improve crystal quality using additives or ligand etc,

your long axis is very worrying as well!!! may be some magic additive could 
change the packing and givew better diffraction too

I also had crystals growing from MPD, unfortunately there was a non-cleavable 
his-tag 
so I could not hope that metals would help. and other additives did not 
make any difference
then my contract finished and so did the project!

I hope you still have a lot of time available to try different things but I 
would not waste time trying to change your cryo

ciao

Stefano

Stefano Benini PhD
AstraZeneca UK
Structural Biology 
Mereside 50S38
Alderley Park








-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of
Patrick Shaw Stewart
Sent: 11 July 2007 12:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity


Just a thought, Mary - going back to your original question about MPD.  I 
extracted the crystallization conditions from REMARK 280 of 3939 PDB entries a 
couple of years ago.  The average concentration of the MPD used was high - 
38.6%, while PEGs tended to be used at lower concs, e.g. PEG400 25.7%.  You can 
see the data at www.douglas.co.uk/top14.htm 

I thought this information could be useful if you want to replace some of the 
MPD with another precipitant (or cryoprotectant).

Best wishes

Patrick Shaw Stewart


--  
[EMAIL PROTECTED] Douglas Instruments Ltd.  
DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK 
Directors: Peter Baldock, Patrick Shaw Stewart, James Smith 
http://douglas.co.uk or http://www.douglasinstruments.com 
Tel: 44 (0) 148-864-9090    US toll-free 1-877-225-2034 
Regd. England 2177994, VAT Reg. GB 480 7371 36


 -Original Message-
 From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Mary
 Fitzgerald
 Sent: 09 July 2007 23:05
 To: CCP4BB@JISCMAIL.AC.UK
 Subject: [ccp4bb] Help with reducing crystal mosaicity
 
 Help please!
 
 I'm looking for some new ideas.  I have crystals that come out of a
 sitting drop with a mixture of sodium cacodylate at pH 6.5, magnesium
 acetate and MPD for the well solution.  The MPD concentration is
 sufficient to act as a cryoprotectant.  Currently, I directly freeze
 these crystals in liquid nitrogen.  When I collect data, I typically
 have high anisotropic mosaicity; it ranges from 0.8 to 1.2.  This is
 further complicated with a weakly diffracting crystal (4-5 A) that has
 a long unit cell axis of ~500 and often twinning.
 
 It has been suggested to me that the cryoprotectent is a problem.  I
 haven't checked the diffraction at room temperature, yet.  Please no
 suggestions of finding a different crystal form as that's not a
 consideration at the moment.  I have my reasons.  I did find one
 crystal that has lower mosaicity (0.5 to 0.8) but had weaker
 diffraction then the typical crystal.  Attempts at flash cryoannealing
 have not helped.
 
 So, what's a good way to change the cryoprotectant if the
 cryoprotectant is the precipitant?  I've considered trying dehydration
 but wasn't certain if that would help with the mosaicity.
 
 Thanks for any ideas,
 
 Mary X. Fitzgerald
 Postdoctoral Associate

Re: [ccp4bb] How to remove nucleic acid contamination for crystallizing zinc finger protein

2007-07-05 Thread Benini, Stefano

Dear Tiancen,

a friend of mine succesfully used a partial denaturation approach using urea to 
get rid of bound DNA, see following citation:


Acta Cryst. (1998). D54, 1043-1045[ doi:10.1107/S0907444998000341 ]

Cloning, overproduction, purification and crystallization of the DNA binding 
protein HU from the hyperthermophilic eubacterium Thermotoga maritima

may be it works on  zinc finger proteins as well

ciao

Stefano



-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of
[EMAIL PROTECTED]
Sent: 05 July 2007 15:43
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] How to remove nucleic acid contamination for
crystallizing zinc finger protein


We have used the PEI precipitation described by Pavan, very high salt (2M or 
even higher) as alluded to by Ana, or heparin columns with success.  In some 
cases, a denaturing purification protocol can be very useful, but of course 
this assumes you can refold the protein.  In the case of a student whose thesis 
committee I am on, the RNase and DNase has not worked, maybe because the NAcid 
is protected by being bound to the protein, although I know others have used it 
with success.  For that student, the ONLY thing that worked was PEI.  

Jeff
___
Jeffrey S. Kieft, Ph.D.
Assistant Professor
Dept. of Biochemistry and Molecular Genetics
University of Colorado School of Medicine
 
http://www.uchsc.edu/sm/bbgn/kieftj.htm
http://www.evolutionarygenomics.com/CERT/CERT.html
_
For mail:
UCHSC at Fitzsimons
Mail Stop 8101, PO Box 6511
Aurora, CO 80045
 
For courier/packages:
South Building RC-1, Room 9110
12801 East 17th Ave.
Aurora, CO 80010
 
phone:  303-724-3257
fax:  303-724-3215
email:   [EMAIL PROTECTED]
 
Open your eyes.  You have only to see things clearly, to understand.
-Leonardo da Vinci
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of William Scott
Sent: Thursday, July 05, 2007 8:04 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] How to remove nucleic acid contamination for 
crystallizing zinc finger protein

It can also ppt out your protein, if it is bound to the DNA.

You could also DNase and RNase the bejesus out of it, and temporarily
unfold the protein to aid in release of the nucleic acids. Then you need
to get rid of these evil enzymes before you put back your nucleic acid of
choice.

Pavan wrote:
 You could also try polyethyleneimine (Polymin P) precipitation to
 precipitate out your DNA. Add Polymin P dropwise  to a final
 concentration of 0.24% to your cell lysate while stirring in the cold
 for an hour. This should precipitate out your DNA. Then centrifuge
 your lysate and use the relevant fraction (supernatant or pellet) for
 further purification.

 Pavan

 On 7/5/07, Ana Silva [EMAIL PROTECTED] wrote:
 Hi,

 How much salt do you have in your protein buffer?
 I would try to increase the salt concetration, during purification.

 Hope it helps.
 Ana



 [EMAIL PROTECTED] wrote:
 
  Dear all,
 
  Sorry for the off-topic question.
 
  I am purifying a zinc finger transcription factor for crystallization.
  The protein appeared as a single band on SDS-PAGE (MW 44KD) after NTA
  chelating column, but its OD280/OD260 ratio is as high as 1.0. So I
  doubt the protein is nucleic acid contaminated, probably because of
  the zinc finger. I tried to remove the nucleic acid by Mono Q and
  Superdex 75 pg, but failed. So could any one recommend some method to
  remove the nucleic acid during protein crystallization, esp. zinc
  finger protein? Any experience or references will be appreciated.
 
  Thanks a lot!
 
  Tiancen Hu
 
  Shanghai Institute of Materia Medica
 
 
 
  
  Ò»Æð À´£¬150 Íò ÈË Í¬ Ê± ÔÚ Íæ µÄ ÃÎ »Ã Î÷ ÓÎ
  http://event.mail.163.com/chanel/xyq.htm?from=126_NO4



 --
 Pavan
 http://umsis.miami.edu/~pvaidyan

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Re: [ccp4bb] How to remove nucleic acid contamination for crystallizing zinc finger protein

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