Re: [ccp4bb] Manual setting of config file in PyRx

[Magnus Wolf-Watz]

Postdoctoral scholarship (2 years) within Structural Biology of Switchable 
Proteins from Directed Evolution

The Magnus Wolf-Watz laboratory has an opening for a two-year postdoctoral 
scholarship. We are searching enthusiastic candidates that will focus on 
structure determination and mechanistic dissection of proteins with either NMR 
spectroscopy, molecular dynamics simulations or x-ray crystallography. The 
scholarship is full-time for two years with access April 1:st, 2024 or by 
agreement. Last day to apply is February 12 2024.

The scholarship is aimed at a project with a unique combination of directed 
evolution and structural biology focused on development of switchable binders 
towards either; (1) cancer linked receptors and/or (2) purification of 
therapeutic antibodies. The binders are based on small soluble scaffold 
proteins (6-8 kDa) that has been subjected to both negative and positive 
selective pressures in order to render them binding-competent only in the 
presence of a metal ion.  Directed evolution is performed using either 
bacterial display or phage display and the aim of the project is to dissect the 
structural and molecular mechanism that underlies the switchable binding 
capacity. The project is a collaboration with Professor Sophia Hober at the 
Royal Institute of Technology (KTH) in Stockholm.

For inquiries please contact 
magnus.wolf-w...@umu.se<mailto:magnus.wolf-w...@umu.se>

Please submit your application at: 
https://www.umu.se/en/work-with-us/postdoctoral-scholarships/6-37-24/



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[ccp4bb] Group Leader Position

[Magnus Wolf-Watz]

The Laboratory for Molecular Infection Medicine Sweden, MIMS, is looking for an 
excellent young researcher to become Group Leader for innovative research in 
molecular infection medicine. The topic includes but is not limited to research 
on the biology of bacteria, viruses, parasites and the microbiome and how these 
interact with the immune system of the host. MIMS has a strong track record of 
research into microbial (including viral) pathogenesis, and provides excellent 
access to national research infrastructure, including cutting edge 
cryo-electron microscopy.

For more information and to submit an application please visit:
https://www.mims.umu.se/jobs-fellowships/2139-open-position-group-leader-researcher-in-molecular-infection-medicine.html

Contact: MIMS Director, Professor Oliver Billker: 
oliver.bill...@umu.se<mailto:oliver.bill...@umu.se>

---
Professor Magnus Wolf-Watz
Vice head of department (research strategies)
Chemistry Department
Linnaeusväg 10
Umeå University
90187 Umeå
Sweden

Cell-phone: 0768 262053
www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/<http://www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/>

-





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[ccp4bb] Post doctor in NMR-based structural biology

[Magnus Wolf-Watz]

Post doctor in NMR-based structural biology

A postdoc position in solid-state/liquid structural NMR is opened in the groups 
of Gerhard Gröbner and Magnus Wolf-Watz, University of Umeå, Sweden. The 
position has the main focus on structure determination of membrane-bound 
proteins of the Bcl-2 family involved in apoptosis but also has a component 
with human protein kinases or small molecule kinases. Depending on the 
candidate the project may also include structural studies with single particle 
cryo EM. The position and more info is found on the website 
(www.umu.se/en/work-with-us/open-positions/) and named "Post doctor in 
NMR-based structural 
biology". Do 
not hesitate to contact Gerhard Gröbner 
(gerhard.grob...@chem.umu.se<mailto:gerhard.grob...@chem.umu.se>) or Magnus 
Wolf-Watz (magnus.wolf-w...@umu.se<mailto:magnus.wolf-w...@umu.se> )

Best wishes
Gerhard and Magnus




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[ccp4bb] Faculty positions in structural biology, Sweden

[Magnus Wolf-Watz]

Tenure track positions in structural biology


Umeå University are announcing tenure track positions in structural biology.  
The positions are open for all classic directions in structural biology and 
including (but not limited to), in situ structural biology,  mass-spec driven 
structural biology and modelling. Umeå University offers a unique and 
comprehensive and state-of-the-art structural biology infrastructure including 
cryoEM, NMR, high performance computation and x-ray crystallography 
(www.biostruct.umu.se).



For more information and to apply please visit:



https://www.umu.se/en/work-with-us/open-positions/up-to-five-tenure-track-positions-as-assistant-professor_446404/


Application deadline February 15, 2022

For inquires please contact
Professor Magnus Wolf-Watz


---
Dr. Magnus Wolf-Watz
Assistant head of department (research strategies)
Professor
Umeå University
Chemistry Department
Linnaeus väg 8
907 36
Umeå
Sweden

Integrated Structural Biology (ISB), Twitter @ISB_UmU
Phone:+46 90 786 76 90
Lab Home Page: 
www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/<http://www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/>
Twitter: @watz_wolf





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[ccp4bb] Phd and postdoc positions in Structural biology

[Magnus Wolf-Watz]

Hi

The Magnus Wolf-Watz lab in Umeå, Sweden has two openings in structural biology:

One PhD position directed at human protein kinases and their linkage to human 
cancer application deadline May 20

One Postdoc in structural biology of silk and cellulose modifying enzymes, 
application deadline April 15!

See below and www.biostruct.umu.se for more information

PhD position in biochemistry; structural biology of cancer linked protein 
kinases

A PhD position in biochemistry is available in the laboratory of Professor 
Magnus Wolf-Watz at the Department of Chemistry, Umeå University, Sweden. The 
PhD project is aimed at uncovering novel molecular mechanisms underlying the 
function of human protein kinases. Focus will be on two specific protein 
kinases that are intimately linked to a number of disorders in humans including 
various forms of cancer. The doctoral student will enter a multidisciplinary 
project that is built on collaborations with both Professor Kwangho Nam at 
University of Texas Arlington and with Marené Landström (Professor in pathology 
at Umeå University). The doctoral student will be trained in structural 
biology, protein production, biochemistry and biophysics and will collaborate 
with both cell biologists and computational chemists. Structural biology may go 
in any of the directions; single particle cryo EM, x-ray crystallography or 
protein NMR spectroscopy depending on the development of the research project 
and also depending on the interests of the doctoral student. The position is in 
part funded by the National Institute of Health (NIH).
2-year postdoc fellowship: Green Structural Biology in cellulose and silk 
biotechnology
An important aspect of green chemistry and a sustainable and circular economy 
is to develop environmentally friendly methodology to retrieve valuable 
chemicals from complex biomaterials. This interdisciplinary project tackles 
this challenging task by exploring the molecular mechanisms underlying 
enzymatic processing of recalcitrant natural polymers: cellulose and silk 
fibers. In the project we focus on two enzymes that bring novel functionality 
in cellulose and silk bio-processing by softening their structure and paving 
the way for further biocatalytic transformation of the fibers.
We are seeking a structural biologist with a strong background in biochemistry 
and/or protein production with different expression organisms. The structural 
biology background may be in any of the areas of NMR spectroscopy, single 
particle cryo EM or x-ray crystallography.   The successful candidate will have 
the possibility to define and shape the project dependent on background and 
ambitions and may focus on both enzymes and/or the structures of cellulose and 
silk-based biomaterials.


Please contact Magnus W-W for further information and guide to the application,


---
Dr. Magnus Wolf-Watz
Professor
Umeå University
Chemistry Department
Integrated Structural Biology (ISB), Twitter @ISB_UmU
Phone:+46 90 786 76 90
Lab Home Page: 
www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/<http://www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/>
Twitter: @watz_wolf





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[ccp4bb] PostDoc program in Integrated Structural Biology @Umeå University

[Magnus Wolf-Watz]

Postdoctoral Program in Integrated Structural Biology at Umeå University

Two postdoctoral fellowships are open for the 2019 call at the Integrated 
Structural Biology (ISB) environment at Umeå University.  Umeå University 
offers a vibrant and international atmosphere for structural biology research. 
There are 19 research groups and close to 100 persons affiliated with ISB. The 
ISB environment has regular seminars and candidates will be broadly exposed to 
different structural biology techniques. The university has state of the art 
infrastructure for cryoEM, NMR spectroscopy, supercomputing and x-ray 
crystallography (please visit www.biostruct.umu.se 
for details on equipment).

The openings will be filled using a procedure adapted from EMBL and the two top 
candidates that has applied to one of the six available projects will be 
selected. The projects are interdisciplinary and will ensure that the candidate 
will receive competitive training.

The program is open to all nationalities with relevant doctoral level education 
and work experiences and the openings will include the following to be 
considered by potential candidates:
·   Two years funding (incl. running costs) for research within a 
multidisciplinary structural biology environment
·   Access to ISB affiliated core facilities and technical platforms
·   Integration with postdocs at ISB and also MIMS (Molecular Infection 
Medicine Sweden)  for carrier development and joint activities

Instructions
Candidates should (in addition to submitting their CV, publications, 
certificates, etc.) write a “MOTIVATION LETTER” (1-2 pages) specifying: i) why 
you are interested in performing postdoc research studies within ISB and ii) 
why in particular you wish to do this with the PI:s and project idea selected 
from our list The applicants must also write a one-page research plan for the 
project. We encourage the applicants to contact the PIs for discussion of the 
project before submission. Please email your application to i...@umu.se no 
later than October 15. For inquires please contact the indicated lead-PI or 
co-PI’s from the list of projects. Further info about the ISB Postdoctoral 
program can be found here: 
https://www.biostruct.umu.se/isb-postdoctoral-program/

PROJECTS
Below please find a list of the available projects together with contact 
information to PI’s.

Mechanistic origin of ATP-dependent ion transport
Computational methods can be used to reveal connections between subtle trigger 
events and large-scale conformational changes in proteins. In this project, 
computer simulations and time-resolved X-ray solution scattering experiments 
(TR-WAXS) will be used to identify such connections in ATP-dependent membrane 
protein transport. Specifically, classical MD and QM/MM simulations will 
characterize effects of phosphorylation, protonation/deprotonation, and ion 
binding in P-type ATPase ion transport. Existing high-resolution protein 
structures as well as low-resolution models of transient states in solution 
(obtained from time-resolved X-ray scattering experiments) will serve as the 
basis for the simulations. The project aims to determine structural dynamics 
and corresponding free energy levels to provide a structural/thermodynamic 
basis for understanding ATP-driven ion transport across biological membranes. 
The project will be lead by Dr. Andersson (classical MD/X-ray scattering) and 
Dr. Nam (QM/MM). Part of the project will take place at University of Texas, 
Arlington, USA.
Lead PI: Magnus Andersson: magnus.p.anders...@umu.se
Co-PI:   Kwangho Nam: kwangho@umu.se

Mechanistic study of the autophagic protein ATG8/LC3 in membrane morphogenesis
Autophagy is a highly conserved and sophisticated “self-eating” process in 
eukaryotes and plays a key role in human health and disease. Formation of 
double-membrane autophagosomes is the key process in autophagy and the LC3 
protein family is required for autophagosomal membrane expansion and closure. 
However, the molecular mechanisms of these processes remain unclear. In this 
project we will focus on the membrane interactions of the LC3-PE protein. Wu’s 
lab have prepared LC3-PE by semisynthetic approaches and showed that LC3-PE 
promotes membrane fusion in vitro . The protein will here be studied using 
structural biology tools, primarily NMR spectrosocpy, together with Mäler’s 
group. The proposed project provides an excellent opportunity for combining 
Wu’s expertise concerning protein chemistry with biophysical and structural 
biology (NMR) studies of the interaction between one key component in autophagy 
and lipids in Mäler’s group.
Lead PI: Lena Mäler: lena.ma...@umu.se
Co-PI:  Yaowen Wu:  yaowen...@umu.se

Functional and structural analyses of the dynamic conformational changes of 
Opsins upon photo responsiveness
Opsins, including Opn3 and Opn5, are G-coupled receptors that detect light by 

[ccp4bb] Postdoc in enzyme dynamics

[Magnus Wolf-Watz]

Dear All

The laboratory of Magnus Wolf-Watz at Umeå University is searching a motivated 
postdoc with an interest in enzyme dynamics. We use diverse techniques to 
address enzymatic rate enhancements from the perspective of enzyme dynamics. 
The candidate should either hold or expect to hold a Ph.D. in the area of 
biochemistry or structural biology, with expertise in either NMR spectroscopy 
or X-ray crystallography. Our department is equipped with state of the art NMR 
spectrometers including an 850 MHz machine equipped with a cryo probe. 
Expertise in protein production and purification is required.

The postdoc position is a fellowship for 2 years. Please visit the lab website 
for more information: 
www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/<http://www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/>.
Send your CV with a brief research statement as well as 2-3 contacts for 
references to magnus.wolf-w...@umu.se<mailto:magnus.wolf-w...@umu.se>  before 
the 1st of August

Thank you,
Magnus Wolf-Watz


---
Dr. Magnus Wolf-Watz
Professor
Umeå University
Chemistry Department
Phone:+46 90 786 76 90

Lab Home Page: www.biostruct.umu.se/principal-investigators/magnus-wolf-watz/




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