[ccp4bb] Up to two postdoctoral positions in integrative structural biology study of signaling complexes

[Page,Rebecca]

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Postdoctoral positions in integrative structural biology study of signaling 
complexes


The Page Laboratory at the University of Connecticut Health Center has an 
immediate opening for up to two postdoctoral candidates to study eukaryotic 
signaling enzymes, with a focus on the function of phosphatases that form large 
signaling complexes and phosphatase drug/inhibitor development (recent 
publications include Nature 
[https://www.nature.com/articles/s41586-023-06870-3], Nature communications 
[https://www.nature.com/articles/s41467-023-37372-5]).


All studies combine cryo-EM, NMR and/or X-ray crystallography with biochemical 
and cellular experiments, allowing postdocs to augment their training in those 
techniques that are new. Applicants with strong backgrounds in biochemical 
techniques, and especially eukaryotic protein expression and protein 
crystallization or cryoEM, are encouraged to apply.


The Page laboratory is housed in a newly renovated ~250,000 sq. ft. research 
laboratory in Farmington, CT. The laboratories are open-lab-space facilities 
and allow close interaction with the adjunct structural biology and 
molecular/cell biology groups at UConn Health. The Page laboratory is equipped 
with all necessary infrastructure to produce (E. coli, mammalian, insect), 
purify and analyze protein and protein complexes (ITC, SPR etc.) and 
crystallization robotics for crystal production (Art Robbins Gryphon LCP 
nano-liter crystallization robot; Art Robbins CrysCam) as well as all needed 
equipment for cryo-EM sample preparation and data evaluation.


UCHC facilities include a Bruker Venture D8 system (IμS Diamond source) with a 
Photon III M14 detector and cold stream is available for crystal screening and 
data collection for in-house data collection, synchrotron access at NSLS-II, 
SSRL, Diamond and high-field NMRs (Bruker Avance Neo 600 MHz and 800 MHz NMR 
spectrometers). Cryo-EM instrument includes a TFS Vitrobot IV and a TFS 
Tundra/Falcon C) that allows for rapid screening of auto-grid/clipped samples 
of sample conditions. Access to Krios instruments for data collection is 
readily available.


UCHC has a welcoming, collaborative and vibrant environment for structural, 
biochemical and translational research. Farmington, CT, is located in New 
England between Boston (90 min.) and New York City (90 min). Laboratory alumni 
are successful, including alums that have their own academic faculty positions, 
are directors/chief scientists in pharma and startups, gone into public policy, 
among others.


Starting date, salary and project details are very competitive and negotiable.


Please e-mail a Cover Letter and CV to Rebecca Page (rp...@uchc.edu)

Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030
rp...@uchc.edu

Page laboratory website



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[ccp4bb] Meeting Invitation: Motifs, modules, networks: Assembly and organization of regulatory signaling systems, July 10-14

[Page,Rebecca]

Dear CCP4 community,

Please join us for the ASBMB sponsored conference:

Motifs, modules, networks: Assembly and organization of regulatory signaling 
systems

Oral and poster presentations: abstract deadline May, 10, 2023 (see below).

https://www.asbmb.org/meetings-events/motifs-modules-networks
[https://www.asbmb.org/getmedia/3191a7e0-bec8-4432-8f48-33f286072bc7/1000x500-motifs-modules-networks.jpg?width=1000=500=.jpg]
Motifs, modules, networks: Assembly and organization of regulatory signaling 
systems
July 11–14, 2023 | Potomac, Md.
www.asbmb.org

July 11–14, 2023
Bolger Center, Potomac, Md.


Important dates
May 10  Early registration deadline*
May 10  Abstract submission deadline for oral and poster (authors are required 
to register for the conference upon submission of abstract)
June 12 Deadline for cancelations/refunds (no refunds after this date)
June 12 Regular registration deadline*

*Registration and housing are on a first-come, first-served basis and will 
remain open until capacity is reached. This may mean that the conference 
registration closes before the officially posted registration deadline. To 
secure your spot at the conference we encourage you to register early.


Meeting focus:

Our understanding of how cellular signaling networks are organized has evolved 
substantially in recent years. Namely, the myriad components of signaling 
networks — receptors, signaling enzymes such as phosphatases and kinases, 
substrates and scaffolding proteins — often assemble through highly dynamic 
interactions involving intrinsically disordered regions and short linear 
sequence motifs (SLiMs). These dynamic interactions allow for rapid responses 
to signaling inputs and dictate systems-level properties of signaling networks. 
Understanding SLiM-mediated interactions has made it possible to define 
“substratomes” of signaling enzymes as well as interaction networks that 
provide novel insights into signaling platforms and cellular networking 
mechanisms.

This interdisciplinary conference will bring together researchers in structural 
biology, biochemistry, computational biology and proteomics who investigate 
cellular signaling networks and leverage these insights into the development of 
new therapeutic strategies.

Best regards,
Rebecca Page
UCHC



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[ccp4bb] Post-doctoral positions available using X-ray crystallography, Cryo-EM and NMR to study signaling complexes

[Page,Rebecca]

Post-doctoral positions for the study of signaling complexes using X-ray 
crystallography, Cryo-EM and NMR.


The Page Laboratory at the University of Connecticut Health Center has an 
immediate opening for up to two post-doctoral candidates to study eukaryotic 
signaling enzymes, with a focus on the function of phosphatases that form large 
signaling complexes and PPP drug/inhibitor development (SHP2, PPPs, PhosTacs). 
All studies combine X-ray crystallography and/or cryo-EM/NMR with biochemical 
and in situ experiments, allowing post-docs to augment their training in those 
techniques that are new. Applicants with a strong backgrounds in biochemical 
techniques, and especially eukaryotic protein expression and protein 
crystallization, are encouraged to apply.


The Page laboratory is housed in a newly renovated ~250,000 sq. ft. research 
laboratory in Farmington, CT. The laboratories are open-lab-space facilities 
and allow close interaction with the adjunct structural biology and 
molecular/cell biology groups at UConn Health. The Page laboratory is equipped 
with all necessary infrastructure to produce (E. coli, Sf9, mammalian), purify 
and analyze protein and protein complexes (ITC, SPR etc.) and crystallization 
robotics for crystal production (Art Robbins Gryphon LCP nano-liter 
crystallization robot; Art Robbins CrysCam).


Outstanding UCHC facilities include a Bruker Venture D8 system (I?S Diamond 
source) with a Photon III M14 detector and cold stream is available for crystal 
screening and data collection for in-house data collection, synchrotron access 
at NSLS-II, SSRL, Diamond and high-field NMRs (Bruker Avance Neo 600 MHz and 
800 MHz NMR spectrometers (installed in 2021). Both spectrometers are equipped 
with four independent channels for 1H, 13C, 15N and 2H observation and 
decoupling and TCI-HCN cryo-probes (1H, 13C, 15N cooled).


UCHC has a welcoming, collaborative and vibrant environment for structural, 
biochemical and translational research. Farmington, CT, is located in New 
England between Boston (90 min.) and New York City (90 min). Starting date, 
salary and project details are negotiable.


Please e-mail a Cover Letter and CV to Rebecca Page (rp...@uchc.edu).



Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030
rp...@uchc.edu

Page laboratory website



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[ccp4bb] Tenure-track Assistant or Associate Professor in Molecular Biophysics at UConn Health

[Page,Rebecca]

Tenure-track Assistant or Associate Professor in Molecular Biophysics at UConn 
Health

The Department of Molecular Biology and Biophysics (MBB) at the University of 
Connecticut Health Center (UConn Health) seeks exceptionally creative 
candidates for an early-to-mid career position (tenure-track Assistant or 
Associate Professor), who use molecular and translational approaches to 
elucidate the molecular basis of disease.

Research in MBB integrates multiple approaches to molecularly define the 
proteins and pathways affected in human disease. We are especially interested 
in creative and innovative candidates whose research interests focus on the use 
of cryo-EM and/or other structural biology methods (X-ray Crystallography, NMR 
spectroscopy, single molecule techniques) to examine key biological questions 
in microbiology, host-pathogen interactions, molecular signaling (including 
areas such as posttranslational modifications, protein ubiquitination, 
kinase/phosphatase signaling/regulation, bacterial signaling, among others) or 
other critical biological processes. The candidate will augment existing 
strengths in the department and the larger UConn Health research community. 
Programs that focus on the identification and development of translational 
outcomes, including probes that will allow for in-depth analysis of key 
biological pathways or novel drug-like molecules, are also of particular 
interest.

MBB and UConn Health provide a rich and diverse research environment and is 
deeply committed to the development and success of its incoming faculty. The 
UConn Health School of Medicine is home to 30 departments and institutes, 
including Cell Biology, Genetics and Genome Sciences, the Richard D. Berlin 
Center for Cell Analysis and Modeling, among others. It is also closely 
affiliated with The Jackson Laboratory, which is located on the same campus. 
Candidates will also have access to 22 exceptional institutional core 
facilities, including the CCAM Microscopy, NMR Structural Biology and 
Biophysics, High-Performance Computing, EM, MS, FACS and Histology​

Successful applicants will have a PhD, MD or equivalent degree; a sustained 
record of exceptional scholarly success; and the promise of future innovative 
accomplishments via a proposed (early-career) or established (mid-career, 
including evidence of successful extramural grant writing) research programs 
that utilize state-of-the-art molecular, genetic, cellular, biochemical, 
biophysical and/or structural methodologies. Candidates with an existing 
funding record are preferred. Candidates are also expected to be highly 
collaborative and integrate with the growing number of researchers and 
physician scientists in the basic and clinical departments at UConn Health.

UConn Health offers exceptional health benefits, retirement benefits and also 
tuition waivers for dependents. UConn Health is situated in the beautiful and 
affordable Farmington River Valley near Hartford, CT, only 2 hours from Boston 
and New York City.

Interested individuals should submit:

  *   a curriculum vitae
  *   cover letter
  *   statements of research (up to 3 pages) and teaching (up to 1 page)

To apply, go to https://jobs.uchc.edu/​ and search for the position by 
selecting the "Molecular Biology and Biophysics" department. The search number 
is 2022-330.

Review of applications will begin November 15, 2021. For inquiries about the 
position, please contact Dr. Wolfgang Peti, Chair of the Search Committee 
(p...@uchc.edu).

For more information regarding the Department of Molecular Biology and 
Biophysics, please visit the department website at 
https://health.uconn.edu/molecular-biology-biophysics/.


Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030



Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030
rp...@uchc.edu

Page laboratory website



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[ccp4bb] Structural Biology faculty position, UConn Health Center (UCHC), assistant/associate professor

[Page,Rebecca]

UCONN Health

ASSISTANT/ASSOCIATE PROFESSOR - BASIC SCIENCES


The Department of Molecular Biology and Biophysics (MBB) at UConn Health, 
Farmington, CT, seeks exceptionally creative candidates for an early-to-mid 
career position (tenure-track Assistant or Associate Professor), who use 
molecular and translational approaches to elucidate the molecular basis of 
disease.



Research in MBB integrates multiple approaches to molecularly define the 
proteins and pathways affected in human disease. Our studies span the 
structural biology of cancer drug targets to the mechanistic interplay between 
viruses and human cells. We seek creative and innovative candidates whose 
research interests and accomplishments complement and further existing 
strengths in the department and the larger UConn Health research community. 
Ideal candidates will have a research program focused on molecular signaling 
(including but not limited to areas such as posttranslational modifications, 
DNA repair, protein ubiquitination, kinase/phosphatase signaling/regulation) 
and/or the structure, function and regulation of proteins involved in signaling 
processes. Programs that focus on the identification and development of 
translational outcomes, including probes that will allow for in-depth analysis 
of key biological pathways or novel drug-like molecules, are also of particular 
interest.



MBB and UConn Health provides a rich and diverse research environment and is 
deeply committed to the development and success of its incoming faculty. The 
UConn Health School of Medicine is home to 30 departments and institutes, 
including Cell Biology, Genetics and Genome Sciences, the Richard D. Berlin 
Center for Cell Analysis and Modeling, among others. It is also closely 
affiliated with The Jackson Laboratory, which is housed on the same campus. 
Candidates will also have access to 22 exceptional core facilities, including 
the CCAM Microscopy, NMR Structural Biology and Biophysics, High-Performance 
Computing, EM, MS, FACS and Histology core facilities. UConn Health also offers 
competitive salaries and excellent benefits.



Successful applications will have a PhD, MD or equivalent degree; a sustained 
record of exceptional scholarly success; and the promise of future innovative 
accomplishments via a proposed (early-career) or established (mid-career, 
including evidence of successful extramural grant writing) research programs 
that utilizes state-of-the-art molecular, genetic, cellular, biochemical, 
biophysical and/or structural methodologies. Candidates are also expected to be 
highly collaborative and integrate well with the growing number of researchers 
and physician scientists in the basic and clinical departments of UConn Health. 
Candidates with ongoing funded research are encouraged to apply.



Please visit https://jobs.uchc.edu/CSS_External/CSSPage_Welcome.asp and select 
'Molecular Biology and Biophysics' under 'department' (job number 2021-650).

?

To apply, interested individuals should submit a curriculum vitae, cover 
letter, statements of research plans (up to 3 pages) and teaching (up to 1 
page) and arrange for the submission of three letters of reference on 
letterhead with signature.



Questions regarding this position should be addressed to Wolfgang Peti, 
Department Head, Molecular Biology & Biophysics at 
p...@uchc.edu.



UConn Health is an affirmative action employer, in addition to an EEO and 
M/F/V/PWD/PV employer.




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[ccp4bb] Post-doctoral position available

[Page,Rebecca]

The Page Laboratory at the University of Connecticut Health Center has an 
immediate opening for up to two post-doctoral candidates to study pro- and 
eukaryotic signaling enzymes, with a focus on the function of phosphatases that 
form large signaling complexes, and also drug/inhibitor development. All 
studies use X-ray crystallography and/or cryo-EM/NMR. Applicants with a strong 
background in biochemical techniques, and especially eukaryotic protein 
expression and protein crystallization, are encouraged to apply.


The Page laboratory is housed in a newly renovated ~250,000 sq. ft. research 
laboratory in Farmington, CT. The laboratories are open-lab-space facilities 
and allow close interaction with the adjunct structural biology and 
molecular/cell biology groups at UConn Health. The Page laboratory is equipped 
with all necessary infrastructure to produce (E. coli, Sf9, mammalian), purify 
and analyze protein and protein complexes (ITC, SPR etc.) and crystallization 
robotics for crystal production.


Farmington, CT, is located on the New England between Boston (90 min.) and New 
York City (90 min.). Starting date, salary and project details are negotiable.

Please e-mail a Cover Letter and CV to Rebecca Page (rpage(at)uchc.edu).


Recent publications:

  *   Wang, X., Garvanska, DH, Nasa, I, Ueki, Y, Zhang G, Kettenbach, AN, Peti, 
W, Nilsson, J, Page, R (2020). A dynamic charge-charge interaction modulates 
PP2A:B56 substrate recruitment. eLife. 9. Pii: e55966.
  *   Choy, M.S., Moon, T.M., Ravindran, R., Bray, J.A., Robinson, L.C., 
Archuleta, T.L., Shi, W., Peti, W., Tatchell, K., Page, R. (2019) SDS22 
selectively recognizes and traps metal-deficient inactive PP1. PNAS 116:  
20472-20481.



Rebecca Page, PhD
Professor
UConn Health | Cell Biology (L5080)
263 Farmington Avenue | Farmington, CT 06030
rp...@uchc.edu

Page laboratory website



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[ccp4bb] Postdoctoral Position available at Brown University, Providence, RI

[Page, Rebecca]

The Page Laboratory at Brown University has an opening for a highly
motivated postdoctoral research scientist to undertake structural analysis
of signaling complexes, with a focus on the interactions between
phosphatases, kinases and their regulatory proteins. Most studies typically
employ a combination of x-ray crystallography, NMR spectroscopy and SAXS.
Applicants with a strong background in biochemical techniques and structure
determination are encouraged to apply.

The Page laboratory is housed in a 105,000 sq. ft. research laboratory in
Providence, RI. The laboratories are open-lab-space facilities and allow
for close interactions with the adjunct structural biology and molecular
biology groups at Brown University. Recent upgrades to the Brown University
structural biology infrastructure include new X-ray crystallography
instrumentation (Rigaku FR-E+ SuperBright with VariMax-HF ArcSec optics, a
Saturn 944 CCD detector and ACTOR robotic crystal mounting system), a
BIOSAXS-1000 small angle X-ray scattering instrument, and the addition of
an 850 MHz NMR spectrometer.

Providence, RI, is located on the coast between Boston (45 min.) and New
York City (3 hours). Starting date, salary and project details are
negotiable.

Please e-mail a Cover Letter and CV to Rebecca Page (rebecca_p...@brown.edu),
Brown University, Box G-E4, Providence, RI 02912.

[ccp4bb] Structural Biology Faculty Position, Assistant Professor

[Page, Rebecca]

Dear crystallography community,

I wanted to make the community aware of a new tenure-track faculty position
at Brown University in structural biology. Details about the position are
provided below.

*Structural Biology Faculty Position, Assistant Professor*

The Department of Molecular Biology, Cell Biology and Biochemistry at Brown
University announces the opening of a tenure-track faculty position with a
start date of July 1, 2013. Qualifications include a Ph.D. and/or M.D.
degree with relevant postdoctoral research training and a record of
excellence in research. The successful applicant will be an experimentalist
in the area of X-ray crystallography who will be expected to pursue
an independent, externally-funded research program. The applicant should
demonstrate a commitment to graduate and undergraduate education, and will
have the opportunity to
participate in pre-doctoral training programs within the Division of
Biology and Medicine and the campus at large. Research space will be
provided in a new, state-of-the-art facility with access to X-ray
equipment, crystallization robotics, NMR spectrometers and
biophysical analysis instrumentation, such as ITC, DSC, CD and mass
spectrometry (LTQ Orbitrap), among others. Recent upgrades to the Brown
University structural biology infrastructure include new X-ray
crystallography instrumentation (Rigaku FR-E+ SuperBright with VariMax-HF
ArcSec optics, a Saturn 944 CCD detector and ACTOR robotic crystal mounting
system), a BIOSAXS-1000 small angle X-ray scattering instrument, and
the addition of an 850 MHz NMR spectrometer.

Candidates should submit a curriculum vitae with a complete bibliography, a
3-5 page description of research plans, a 1-page teaching statement and up
to 3 representative publications. These materials should be submitted
electronically to
http://www.interfolio.com/apply/14669. Three confidential reference letters
should be submitted separately to the same website (instructions for
requesting confidential letters of recommendation are also provided on the
same website). Review of applications will commence on *November 1, 2012*,
and will continue until the position is filled.

Brown University is an equal opportunity (EEO/AA) employer and is committed
to increasing the diversity of its faculty. It welcomes nominations and
applications from minorities, women, and individuals with varied
experiences, perspectives and backgrounds, which would enrich the
university's research, teaching and service missions.

[ccp4bb] Brown University Structural Biology Core Facility Manager Position

[Page, Rebecca]

Dear CCP4 community,

Although this position requires expertise in NMR spectroscopy, I wanted to
send the announcement to this community as many crystallographers have
colleagues who are NMR spectroscopists that may be interested in this
position. Please feel free to forward to anyone you think may be interested
and thanks in advance.

Rebecca Page


*Brown University Structural Biology Core Facility Manager*

 *Job Description *

The Division of Biology and Medicine at Brown University seeks a Facility
Manager for its Structural Biology facility, which includes an NMR
facility, as well as a biological crystallography and small angle X-ray
facility. This rapidly growing shared use Facility is located in a research
building adjacent to the main Medical School Building. The NMR facility
consists of 2 spectrometers (both Bruker) operating at 11.7 and 19.97 T.
The X-ray and SAXS facility consists of a combined Rigaku instrument
(dual-port FRE+ anode). The core structural biology community at Brown
consists currently of 3 tenured faculty with an additional tenure track
search in progress. Additional growth in the number of structural biology
faculty is anticipated. Furthermore, the facility and its instruments will
serve any research group at Brown University and beyond. NMR spectrometers
are used for modern NMR experiments on biomacromolecules in solution. The
successful candidate will be responsible for operating and maintaining the
instrumentation, for implementing new NMR experiments and for training and
supervising student and postdoc users. The candidate will be expected to
maintain a broad knowledge of state-of-the-art NMR technology and
equipment.



*Required Experience *

• A Ph.D. in chemistry/physics/biophysics or a related area with four years
of experience in solution NMR spectroscopy and its applications to
biological systems.

• A demonstrated expertise in solution NMR spectroscopy, including
development and implementation of new NMR experiments - in particular
multidimensional experiments for structural analysis of biological systems.

• Knowledge of NMR hardware and software for data collection and processing
in modern solution NMR spectrometers, as well as extensive experience with
computers.

• Knowledge of modern structure and/or relaxation analysis software.

• Excellent communication and organizational skills, the ability to
positively interact with a diverse group of NMR users, and a strong
motivation to learn new NMR techniques.

• An interest in learning new areas of structural biology, specifically for
the upkeep of the X-ray and SAXS instrumentation, as well as training of a
diverse group of users.

• Ability to manage a shared use facility by complying with federal
research guidelines and University fiscal policies governing shared use
facilities.



Applications should be submitted online via Brown’s automated application
system at https://careers.brown.edu/applicants/Central?quickFind=118396.
Applications should include an up-to-date curriculum vitae, three reference
letters, as well as a statement of expertise in regards of all points
mentioned above. Review of applications will begin on Feb 15, 2012, and
will continue until the position is filled. Candidates offered a staff
position at Brown are subject to an employment reference check and
additional background screening satisfactory to Brown University. This
additional background screening may include a criminal background check,
drug testing, health screening and/or motor vehicle records checks.



Brown University is an Equal Opportunity/Affirmative Action employer and
welcomes

[ccp4bb] Assistant Professor in Structural Biology, Brown University

[Page, Rebecca]

Dear colleagues,

I would like to bring your attention to the following vacancy at Brown
University. Please feel free to forward to any NMR structural biology
colleagues that might be interested in this position:

*Assistant Professor in Structural Biology, NMR*

The Department of Molecular Pharmacology, Physiology and
Biotechnologyhttp://brown.edu/academics/molecular-pharmacology-physiology-and-biotechnology/at
Brown University announces the opening of a faculty position at the
rank
of Assistant Professor, effective July 1, 2012. Qualifications include a
Ph.D. and/or M.D. degree with relevant postdoctoral research training and a
record of excellence in research. The successful applicant will be an
experimentalist in the area of NMR spectroscopy who will be expected to
pursue an  independent, externally-funded research program. The applicant
should demonstrate a commitment to graduate and undergraduate education,
and will have the opportunity to participate in predoctoral training
programs within the Division of Biology and Medicine and the campus at
large. Research space will be provided in a new, state-of-the-art facility
with access to modern core facilities, NMR spectrometers, X-ray equipment,
crystallization robotics, and
biophysical analysis instrumentation, such as ITC, DSC, CD and mass
spectrometry (LTQ Orbitrap) among others. Recent upgrades to the Brown
University structural biology infrastructure include the planned addition
of an 850 MHz NMR spectrometer and new X-ray crystallography and small
angle X-ray scattering (SAXS) equipment.

Candidates should submit a curriculum vitae with complete bibliography, a
3-5 page description of research plans, and a one-page teaching statement.
Applicants should arrange for at least three reference letters to be sent
under separate cover. Review of materials will commence on January 15,
2012, and will continue until the position is filled. Materials should be
submitted electronically in PDF format to structural_biol...@brown.edu or
by mail to:
Betsy Jacobson, Department of Molecular Pharmacology, Physiology 
Biotechnology, Brown University, Box G-E3, Providence, RI 02912.


-- 
Rebecca Page
Associate Professor of Biology
Brown University
Dept. of Molecular Biology, Cell Biology and Biochemistry
Center for Genetics, Genomics and Proteomics
70 Ship Street
Providence, RI 02903

[ccp4bb] summary: Home SAXS systems

[Page, Rebecca]

Thanks in advance to everyone to everyone who responded to my previous post
about their experiences doing SAXS at home.

Below is a summary of the responses.

Best,
Rebecca


I am using NANOSTAR BRUKER for few years, and the sealed tube is out of
order
now and the intensity dropped significantly even after rigorous alignment of
beam. However, there might have a better beam source using microfocus beam
that
deliver better flux. Try to think about this. The variable temp sample stage
is
also advantageous and useful for other non-protein samples if needed.




We don't have an in-house SAXS system (yet) but I spent quite some time
looking at different commercial systems (Rigaku, Bruker, Anton-Paar, Hecus).
The two systems I liked the most are from Anton Paar and from
Hecus, both form Graz, Austria. They both build a Kratky camera, which has
the beam focused on the detector and allows for a much smaller footprint
(1-1.5 mtrs sample to detector) compared to a system with a collimated beam
(as used at synchrotrons and with the in-house system from Bruker). Another
advantage of the focused beam is that it doesn't cut off most of the beam so
you get more photons on the sample, giving shorter exposure times.
I sent a protein sample to both companies and also collected
SAXS data on the synchrotron for comparison. The data form the in-house
systems is very similar to synchrotron data, albeit somewhat more noisy.
Exposure time was very decent: 20-30 minutes for a 80 kDa protein at 10
mg/ml using a micro-source. With a modern rotating anode I expect one could
get even shorter exposure times.
As for detectors, Pilatus or a gas detector gave good data.
CCD camera will give more noisy data and an image plate would take too long
to scan in my view.







We've tested other instruments, including the previous system from Rigaku,
and none of them came close to synchrotron data. The original Rigaku pinhole
system was a pain to align and had a kludgy sample system. The AntonParr
system did not give good data at all but we've since heard from an
Australian group that the US reps are not strong on biological samples and
that may be a fluke. We've not tested the Bruker system. Until the
BioSAXS-1000 we'd been very negative about the possibility of a lab based
system. However, the Kratky geometry (with the flux advantage it provides)
coupled to a microfocus system amazed us.  We're frequent synchrotron users
for SAXS but see the ability to characterize samples beforehand as useful.
We're doing a lot of work and in this case a significant proportion of our
data could be collected at home. What is more useful is the ability to
characterize solution or sample conditions for the more difficult targets
before spending time at the synchrotron collecting data to prepare for the
ideal experiment. I'm guessing this is your interest. We're after a home
system for this purpose.







Here’s my two cents on our Home SAXS experience at Penn so far.  Feel free
to post in entirety:

Two years ago our department installed a Rigaku PSAXS
small-angle x-ray scattering system installed on a port of an existing (and
relatively old) RU-H3R rotating anode generator. Currently our setup
includes Osmic mirror optics, a 3-pinhole enclosed pre-flight path, an
evacuated sample chamber with cryostated sample holder, adjustable
sample-to-detector distances, and gas-filled multi-wire detector. Generally,
we leave it in one configuration that provides an accessible scattering
angle of 0.006  Q  ~0.25, sufficient for the characterization of most any
macromolecule.  (where Qmin*Dmax should be less than or equal to Pi to
perform the inverse Fourier transform)

Our group does a lot of synchrotron SAXS and neutron scattering,
as do several groups in our department.  Compared to data from these
sources, the data quality from the home source that can be obtained for a
well-behaved and monodisperse sample can be very good, on par with the
quality of data obtained with neutron scattering (ie publication quality).
 This type of instrument is ideal for binding experiments using I(0) or
basic characterization of a sample for structural examination. (I haven’t
had great luck yet doing I(0) binding experiments at synchrotrons, but I
haven’t given up just yet.)

We’ve done measurements on a variety of systems ranging in size
from 660 kD down to 9 kD – as long as you can find the right concentrations
to work at to get good signal-to-noise, and your sample is well-behaved for
the time course of the experiment, you’re in good shape (pun unintended).
 We generally start out at 3-6mg/mL for things between 45-100kD and take it
from there. Generally, much higher sample concentrations are needed for
small macromolecules with this type of generator.

The sample holder provided by the vendor was a capillary system
(~100 uL per sample).  For us, this didn’t work out for a general user
scheme and for 

[ccp4bb] Home SAXS systems

[Page, Rebecca]

Dear CCP4 bulletin board:

I am doing some research on home SAXS systems (i.e., the BioSAXS from
Rigaku, among others) and was interested in finding out what the community's
experience has been with these systems in performing SAXS experiments at
home. I'll post a summary of all the responses to the bb.

Thanks in advance,
Rebecca

[ccp4bb] Calculation of the net charge of an electrostatic surface for secondary structural elements

[Page, Rebecca]

Dear CPP4 community,

 

I am in search of a program that will calculate the net charge of an
electrostatic surface formed by individual secondary structural elements
(versus the entire protein)  of a structure: for example, the net charge
of the electrostatic surface of helices 2 and 3 versus, say, helices 3
and 4 of the same protein. 

 

 

Thanks in advance,

 

Rebecca 

 

 

[ccp4bb] ITC--favorite instrumentation

[Page, Rebecca]

Dear CCP4 community,

 

We have the possibility of purchasing a new ITC instrument and have been
very interested in the ITC200, which is promoted as an instrument
appropriate for ~10-fold reduction in sample volume. However, I have
also heard that the concentrations required to get good signal are
higher than that of their standard instrument

 

1.   Has anyone used the ITC200 (microcal)? If so, do you see
improved sensitivity? 

2.   Has anyone used the TA instrument nano-ITC
(http://www.tainstruments.com/main.aspx?id=263n=1siteid=11)

 

Thanks so much in advance,

Rebecca

 

 

Rebecca Page

Brown University

Dept. of Molecular Biology, Cell Biology and Biochemistry

 

[ccp4bb] Proper Reference for Phil Evans Program POINTLESS

[Page, Rebecca]

CCP4ers:

 

What is the correct reference for Phil Evans Program POINTLESS?

 

Thanks for your help!

 

Rebecca

[ccp4bb] BOG.cif library file, solved

[Page, Rebecca]

Thanks for all the great responses-

 

Look at the hicup files... 

http://xray.bmc.uu.se/hicup/BOG/

There is also the option to link to the prodrg2 server

which can compute the restraints for you...

 

the above worked perfectly.

 

Rebecca