Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

[Frank von Delft]

We bought some recently hoping for some magic for a specific project 
that we needed new crystal forms for.  Alas, no fairy dust magic in our 
hands.  Maybe we didn't do the protocol right - but of course, one 
doesn't expect to have to do that with fairy dust. We haven't tried with 
many things, though.


All these tricks published over the years seem to /on average/ but 
hardly ever when you need them.  Which is fine if you're funded to 
generate lots of structures, but frustrating if you're funded to crack 
specific science.


While I'm at it:  for those of you asked to review such methods in the 
future, could you insist on them being tested on a lot more than 
lysozyme and thaumatin?


phx


On 31/03/2018 01:44, Whitley, Matthew J. wrote:
I would also be interested in learning whether polyoxotungstate has 
worked wonders for anyone.  At the current price of 387 EUR/gram, it 
is a bit too expensive for us to seriously consider giving it a try.  
Yes, a gram is a lot of compound and would probably last a long time, 
but there are other things at present that we know would give us more 
bang for our buck euro.  If anyone has any great stories of how it was 
a game changer for them, that would do a lot to motivate us to give it 
a try.  Eager to hear about your experiences using the compound.


Thanks,
Matthew
---
Matthew J. Whitley, Ph.D.
Research Instructor
W. Furey Lab
Department of Pharmacology & Chemical Biology
University of Pittsburgh School of Medicine
On 3/30/2018 7:00 PM, CCP4BB automatic digest system wrote:

--
Date:Fri, 30 Mar 2018 20:24:29 +0200
From:Nikolay Dobrev
Subject: Anderson?Evans polyoxotungstate (TEW)

Dear all,
I apologize for my off-topic question.
I want to ask if anyone has so far used the Anderson-Evans
polyoxotungstate
(https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jenabioscience.com%2Fcrystallography-cryo-em%2Fscreening%2Fxp-screen%2Fx-tew-anderson-evans-polyoxotungstate=01%7C01%7Cmjw100%40PITT.EDU%7C27d0177bd91c4d421ed508d59692ef9d%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1=IXVk9RvttYWokRiY2M9H43br5JHo2REjfx9HBML13rI%3D=0).
Did someone see an improvement in crystal quality/diffraction?
Any comments will be highly appreciated.

Nikolay Dobrev



Nikolay Dobrev
PhD Student in AG Sinning & AG Fischer
Biochemie Zentrum
Heidelberg University
Im Neuenheimer Feld 328
69120 Heidelberg
Germany
Phone: +49 6221 54 4796
Email: 

Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

[Linda Miallau]

Y , sj

Le sam. 31 mars 2018 13:21, Patrick Shaw Stewart  a
écrit :

>
> I notice that Molecular Dimensions offers a similar compound
>
> https://www.moleculardimensions.com/shopcontent.asp?type=crystallophoreno1
>
>
> Are there any others?
>
>
>
> On 30 March 2018 at 19:24, Nikolay Dobrev <
> nikolay.dob...@bzh.uni-heidelberg.de> wrote:
>
>> Dear all,
>> I apologize for my off-topic question.
>> I want to ask if anyone has so far used the Anderson-Evans
>> polyoxotungstate (
>> https://www.jenabioscience.com/crystallography-cryo-em/screening/xp-screen/x-tew-anderson-evans-polyoxotungstate
>> ).
>> Did someone see an improvement in crystal quality/diffraction?
>> Any comments will be highly appreciated.
>>
>> Nikolay Dobrev
>>
>>
>> 
>> Nikolay Dobrev
>> PhD Student in AG Sinning & AG Fischer
>> Biochemie Zentrum
>> Heidelberg University
>> Im Neuenheimer Feld 328
>> 69120 Heidelberg
>> Germany
>> Phone: +49 6221 54 4796
>> Email: > 
>>
>
>
>
> --
>  patr...@douglas.co.ukDouglas Instruments Ltd.
>  Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK
>  Directors: Peter Baldock, Patrick Shaw Stewart
>
>  http://www.douglas.co.uk
>  Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
>  Regd. England 2177994, VAT Reg. GB 480 7371 36
>

Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

[Patrick Shaw Stewart]

I notice that Molecular Dimensions offers a similar compound

https://www.moleculardimensions.com/shopcontent.asp?type=crystallophoreno1


Are there any others?



On 30 March 2018 at 19:24, Nikolay Dobrev <
nikolay.dob...@bzh.uni-heidelberg.de> wrote:

> Dear all,
> I apologize for my off-topic question.
> I want to ask if anyone has so far used the Anderson-Evans
> polyoxotungstate (https://www.jenabioscience.co
> m/crystallography-cryo-em/screening/xp-screen/x-tew-anderson
> -evans-polyoxotungstate).
> Did someone see an improvement in crystal quality/diffraction?
> Any comments will be highly appreciated.
>
> Nikolay Dobrev
>
>
> 
> Nikolay Dobrev
> PhD Student in AG Sinning & AG Fischer
> Biochemie Zentrum
> Heidelberg University
> Im Neuenheimer Feld 328
> 69120 Heidelberg
> Germany
> Phone: +49 6221 54 4796
> Email:  
>



-- 
 patr...@douglas.co.ukDouglas Instruments Ltd.
 Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK
 Directors: Peter Baldock, Patrick Shaw Stewart

 http://www.douglas.co.uk
 Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034
 Regd. England 2177994, VAT Reg. GB 480 7371 36

Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

[Whitley, Matthew J.]

I would also be interested in learning whether polyoxotungstate has 
worked wonders for anyone.  At the current price of 387 EUR/gram, it is 
a bit too expensive for us to seriously consider giving it a try.  Yes, 
a gram is a lot of compound and would probably last a long time, but 
there are other things at present that we know would give us more bang 
for our buck euro.  If anyone has any great stories of how it was a game 
changer for them, that would do a lot to motivate us to give it a try.  
Eager to hear about your experiences using the compound.


Thanks,
Matthew

---
Matthew J. Whitley, Ph.D.
Research Instructor
W. Furey Lab
Department of Pharmacology & Chemical Biology
University of Pittsburgh School of Medicine

On 3/30/2018 7:00 PM, CCP4BB automatic digest system wrote:

--
Date:Fri, 30 Mar 2018 20:24:29 +0200
From:Nikolay Dobrev 
Subject: Anderson?Evans polyoxotungstate (TEW)

Dear all,
I apologize for my off-topic question.
I want to ask if anyone has so far used the Anderson-Evans
polyoxotungstate
(https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jenabioscience.com%2Fcrystallography-cryo-em%2Fscreening%2Fxp-screen%2Fx-tew-anderson-evans-polyoxotungstate=01%7C01%7Cmjw100%40PITT.EDU%7C27d0177bd91c4d421ed508d59692ef9d%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1=IXVk9RvttYWokRiY2M9H43br5JHo2REjfx9HBML13rI%3D=0).
Did someone see an improvement in crystal quality/diffraction?
Any comments will be highly appreciated.

Nikolay Dobrev



Nikolay Dobrev
PhD Student in AG Sinning & AG Fischer
Biochemie Zentrum
Heidelberg University
Im Neuenheimer Feld 328
69120 Heidelberg
Germany
Phone: +49 6221 54 4796
Email: 

[ccp4bb] Anderson?Evans polyoxotungstate (TEW)

[Nikolay Dobrev]

Dear all,
I apologize for my off-topic question.
I want to ask if anyone has so far used the Anderson-Evans  
polyoxotungstate  
(https://www.jenabioscience.com/crystallography-cryo-em/screening/xp-screen/x-tew-anderson-evans-polyoxotungstate).

Did someone see an improvement in crystal quality/diffraction?
Any comments will be highly appreciated.

Nikolay Dobrev



Nikolay Dobrev
PhD Student in AG Sinning & AG Fischer
Biochemie Zentrum
Heidelberg University
Im Neuenheimer Feld 328
69120 Heidelberg
Germany
Phone: +49 6221 54 4796
Email: