Re: [ccp4bb] Queries regarding bead beater and french press.
Thanks to all. I noted down all the information you shared with us. Cheers! Johnson L.W On Tue, Jan 13, 2015 at 3:08 PM, Valerie Biou valerie.b...@ibpc.fr wrote: hello we have a French press, a bead machine and constant CellD disruptor in our institute. I can tell you that most people have switched to the Constant system because it works fine and is so much easier and faster to run and clean. people disrupt yeast without any problem. all the best Valerie Le 12 janv. 2015 à 16:44, Johnson Luwang a écrit : Dear All, I am looking for an equipment that can do *Saccharomyces cerevisiae* cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about bead beater for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
Re: [ccp4bb] Queries regarding bead beater and french press.
hello we have a French press, a bead machine and constant CellD disruptor in our institute. I can tell you that most people have switched to the Constant system because it works fine and is so much easier and faster to run and clean. people disrupt yeast without any problem. all the best Valerie Le 12 janv. 2015 à 16:44, Johnson Luwang a écrit : Dear All, I am looking for an equipment that can do Saccharomyces cerevisiae cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about bead beater for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
[ccp4bb] Queries regarding bead beater and french press.
Dear All, I am looking for an equipment that can do *Saccharomyces cerevisiae* cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about bead beater for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
Re: [ccp4bb] Queries regarding bead beater and french press.
[Advertisement on] Avestin Emulsiflex C5 [/Advrtisement off] Google is your friend. Jürgen .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742tel:%2B1-410-614-4742 Lab: +1-410-614-4894tel:%2B1-410-614-4894 Fax: +1-410-955-2926tel:%2B1-410-955-2926 http://lupo.jhsph.edu On Jan 12, 2015, at 10:44 AM, Johnson Luwang jlw_...@iisertvm.ac.inmailto:jlw_...@iisertvm.ac.in wrote: Dear All, I am looking for an equipment that can do Saccharomyces cerevisiae cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about bead beater for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
Re: [ccp4bb] Queries regarding bead beater and french press.
The Bead Beater has a 15, 40, and 350 mL chambers. I haven't used mine to homogenize yeast, but I suspect it is similar in performance to E. coli disruption. (Different bead sizes are used for yeast than bacteria.) We get excellent, gentle disruption of E. coli in 8 minutes total. A French Press takes a loong time for more than 40-50 mL of lysate, and often requires multiple passes for complete disruption. Beads and cell wall debris can be easily removed by centrifugation for direct application on your FPLC. We moved to Bead Beaters from a French Press more than a decade ago and haven't looked back. We can do one liter overexpression preps (20-30 g wet packed cells) in the 40 mL chamber. Cheers, ___ Roger S. Rowlett Gordon Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu On 1/12/2015 10:44 AM, Johnson Luwang wrote: Dear All, I am looking for an equipment that can do /Saccharomyces cerevisiae/ cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about bead beater for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
