Re: [ccp4bb] effect of Izit dye within the crystal structure
Dear Adam and colleagues The enzyme human glutathione reductase (hGR) binds dyes such as safranin and xanthene, which are analogous to methylene blue (aka IZIT) with respect to their heterocyclic ring structures, in a cavity deep at the dimer interface. Safranin and xanthene are also inhibitors of hGR and crystal structures of complexes of GR with safranin and xanthene have been reported: Karplus et al. 1989. Eur J Biochem. 178(3):693-703. Savvides Karplus 1996. Journal of Biological Chemistry 271(14):8101-7. Given the general resemblance of these compounds to methylene blue and just to satisfy my curiosity at the time, I soaked methylene-blue into crystals of GR and collected good data to 2 angs. I was basically able to confirm that methylene blue bound specifically in the cavity at the dimer interface just like the other two compounds. However, this work has remained unreported. best regards Savvas On 11 Nov 2011, at 05:34, adam andres wrote: Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? Cheers Adam Campos
Re: [ccp4bb] effect of Izit dye within the crystal structure
I've collected data from crystal soaked with brightly coloured ligands which partition into the solvent channels. Although the crystal becomes highly coloured (mopping up most of the colour from the drop) the structures remained resolutely empty. I am intrigued by this observation and wonder how mutating the residues that constitute the solvent channels would affect soakbility and other crystal properties. David Hargreaves Associate Principal Scientist _ AstraZeneca DECS, CPSS Mereside, 50F49, Alderley Park, Cheshire, SK10 4TF Tel +44 (0)01625 518521 Fax +44 (0) 1625 232693 David.Hargreaves @astrazeneca.com mailto:name.surn...@astrazeneca.com Please consider the environment before printing this e-mail From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of adam andres Sent: 11 November 2011 04:34 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] effect of Izit dye within the crystal structure Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? Cheers Adam Campos -- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies.
Re: [ccp4bb] effect of Izit dye within the crystal structure
Typically provided that the dye does not kill the crystal, there is no effect on structure, at least nothing I ever saw. The effect is similar to sizing column. Artem On Nov 10, 2011 10:44 PM, adam andres adam_cienc...@yahoo.com wrote: Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? Cheers Adam Campos
[ccp4bb] effect of Izit dye within the crystal structure
Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that presentthis dye? Cheers Adam Campos
Re: [ccp4bb] effect of Izit dye within the crystal structure
I wouldn't be surprised if it is methylene blue. Jürgen On Nov 10, 2011, at 11:34 PM, adam andres wrote: Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? Cheers Adam Campos .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/