I've collected data from crystal soaked with brightly coloured ligands which partition into the solvent channels. Although the crystal becomes highly coloured (mopping up most of the colour from the drop) the structures remained resolutely empty. I am intrigued by this observation and wonder how mutating the residues that constitute the solvent channels would affect soakbility and other crystal properties.
David Hargreaves Associate Principal Scientist _____________________________________________________________________ AstraZeneca DECS, CP&SS Mereside, 50F49, Alderley Park, Cheshire, SK10 4TF Tel +44 (0)01625 518521 Fax +44 (0) 1625 232693 David.Hargreaves @astrazeneca.com <mailto:[email protected]> Please consider the environment before printing this e-mail From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of adam andres Sent: 11 November 2011 04:34 To: [email protected] Subject: [ccp4bb] effect of Izit dye within the crystal structure Hi crystallographers Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? Cheers Adam Campos -------------------------------------------------------------------------- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies.
