It happens.
Ef-Tu is methylated on a single lysine
Also LafV is a putative methylase in E. Coli but its effects were not
studied in detail.
Salmonella methylates flagellin which is how lysine methylation was
discovered - and E.coli is a kissing cousin so...
Artem
On Tue, Nov 5, 2019, 12:31 Jennifer Fleming wrote:
>
> Dear CCP4 community,
>
>
>
> I am hoping that someone here might know the answer to this as I cannot
> find anything concrete in my literature search.
>
>
>
> I have recently received a mass spec result indicating that a recombinant
> protein expressed in *E. coli* is heavily lysine methylated. I would like
> to know is this an artefact and is it even possible for *E. coli* to
> methylate lysines?
>
>
>
> Thank you for any help,
>
>
>
> Jen
>
>
> --
>
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