Hi,
I would only suggest using the brute rotation function if you want to use the
rotate around option or do something other than a full rotation search,
because the fast search followed by rescoring should give you essentially the
same list of peaks (but faster).
If you want to get a bigger list of orientations, you should be able to open
the Expert parameters section of the ccp4i GUI for Phaser, then change the
percent cutoff in the Purge rotation peaks line to something lower than the
default of 75%. Alternatively, if you're running Phaser from a script, you can
include commands like:
PURGE ROT ENABLE ON
PURGE ROT PERCENT 50.0
I've just tested and this works for me. Let me know if you're still having
trouble with this.
Best wishes,
Randy Read
On 5 Dec 2013, at 10:11, LISA science...@gmail.com wrote:
hi all,
I try to solve the crystal structures of a mult-domain protein with phaser. I
cannot solve the structure by automatic search using domain structure as the
models. I tried to do the rotation search using the fast or brute rotation
function. I defined the rotation search peaks number is 1000. But I only get
no more than 100 peaks. I tried the phaser in ccp4-6.3.0 and ccp4-6.4.0. But
I can only get much less peaks than I required in the rotation. But the old
version phaser can give about 1000 peaks if I use brute rotation function ask
for 1000 peaks. How can I more rotation peaks in the new version phaser
?
Thank you.
Lisa
--
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: + 44 1223 336500
Wellcome Trust/MRC Building Fax: + 44 1223 336827
Hills RoadE-mail: rj...@cam.ac.uk
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
