Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-17 Thread luzuok 



You can also try products from Bio-rad.
http://www.bio-rad.com/featured/en/nickel-columns-nickel-resin.html


Best!



--

卢作焜
南开大学新生物站A202


Lu Zuokun, Ph.D. Candidate
College of Life Science, Nankai University

At 2017-02-18 04:31:08, "Kevin Jin"  wrote:

Sorry, the company was CloneTech, now it is Takara (not Takeda). You may talk 
with Dr. Gia Jokhadze, who is the director of protein chemistry department. He 
would give you more information.  You can find his profile on linkedin. He is 
very nice person. Probably, you can ask some free sample from him. 


The advantage of their clone: first, high capacity and better column 
performance(plate numbers). 


On Fri, Feb 17, 2017 at 9:33 AM, Kevin Jin  wrote:

Probably you can try His-Column from Clonetech (Takeda ?). They modified the 
column packing using nylon membrane, which offered better flow-through than 
that of traditional resin-gel column. 






On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger  
wrote:

Dear all,

we are happy users of all that GE offers around their FPLC system, but I am 
getting a little tired of feeling monopolized. Are any of you aware of either 
empty columns or prepacked columns (e.g. metal affinity or ion exchange resins) 
from other companies?

Thanks for your advice


Markus






 





--

Kevin Jin
 
Sharing knowledge each other is always very joyful..
 
Website: http://www.jinkai.org/

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-17 Thread Kevin Jin 
Sorry, the company was CloneTech, now it is Takara (not Takeda). You may
talk with Dr. Gia Jokhadze, who is the director of protein chemistry
department. He would give you more information.  You can find his profile
on linkedin. He is very nice person. Probably, you can ask some free sample
from him.

The advantage of their clone: first, high capacity and better column
performance(plate numbers).

On Fri, Feb 17, 2017 at 9:33 AM, Kevin Jin  wrote:

> Probably you can try His-Column from Clonetech (Takeda ?). They modified
> the column packing using nylon membrane, which offered better flow-through
> than that of traditional resin-gel column.
>
>
>
> On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger <
> markus.seeli...@gmail.com> wrote:
>
>> Dear all,
>> we are happy users of all that GE offers around their FPLC system, but I
>> am getting a little tired of feeling monopolized. Are any of you aware of
>> either empty columns or prepacked columns (e.g. metal affinity or ion
>> exchange resins) from other companies?
>> Thanks for your advice
>>
>> Markus
>>
>
>
>
>
>



-- 
Kevin Jin

Sharing knowledge each other is always very joyful..

Website: http://www.jinkai.org/

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-17 Thread Kevin Jin 
Probably you can try His-Column from Clonetech (Takeda ?). They modified
the column packing using nylon membrane, which offered better flow-through
than that of traditional resin-gel column.



On Wed, Feb 15, 2017 at 8:57 AM, Markus Seeliger 
wrote:

> Dear all,
> we are happy users of all that GE offers around their FPLC system, but I
> am getting a little tired of feeling monopolized. Are any of you aware of
> either empty columns or prepacked columns (e.g. metal affinity or ion
> exchange resins) from other companies?
> Thanks for your advice
>
> Markus
>

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread wangsa tirta Ismaya 
We used cOmplete Histag column from Roche, 5 ml prepacked. We bought 3-4
prepacked columns and a bottle of 25 ml (cheaper). The nice thing about
this Roche pack, if you closed the outlet and let the pump run into the
inlet, the cap will slowly detach .. We use this trick to replace the
resin inside once the performance goes bad. We can also fill it with resin
of other types/brands.
Cheers,
Wangsa

On 15 February 2017 at 23:57, Markus Seeliger 
wrote:

> Dear all,
> we are happy users of all that GE offers around their FPLC system, but I
> am getting a little tired of feeling monopolized. Are any of you aware of
> either empty columns or prepacked columns (e.g. metal affinity or ion
> exchange resins) from other companies?
> Thanks for your advice
>
> Markus
>



-- 

Wangsa Tirta Ismaya
Jakarta, Indonesia
http://wangsatirtaismaya.blogspot.co.id

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread Thomas Cleveland 
I'll second those suggestions and also note that empty XK columns from GE
regularly show up on eBay for pennies on the dollar.

Roch cOmplete is really great in situations where there are chelators
around (especially proteins secreted into eukaryotic tissue culture media).
It saved me tremendous amounts of time in one case, where I no longer had
to concentrate/dialyze liters worth of media but could just filter it and
shoot it through a column.

-Tom

-

Thomas Cleveland, Ph.D.
NIST Center for Neutron Research
100 Bureau Dr. MS 6102
Gaithersburg, MD 20899-6102

On Wed, Feb 15, 2017 at 1:04 PM, Carlos Kikuti  wrote:

> Well GE sells empty columns (XK for low back-pressure, tricorn for higher
> back-pressure) that you can fill with the resin you want. They seem
> expensive but for that kind of things I don't think any option will be real
> 'savings'. For nickel affinity we have been having good results with
> Roche's Complete - it requires less imidazole for binding and elution, and
> at the same time it is compatible with reducing agents and chelators. And
> we still buy the HisTrap FF crude.
>
> On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth <
> christianroth...@gmail.com> wrote:
>
>> Hi Markus,
>>
>> BioRad has columns as well (prepacked and empty), which follow the
>> establiched connector standard. So usable without the need of adapters.
>>
>> Cheers
>>
>> Christian
>>
>> Am 15.02.2017 um 16:57 schrieb Markus Seeliger:
>>
>> Dear all,
>> we are happy users of all that GE offers around their FPLC system, but I
>> am getting a little tired of feeling monopolized. Are any of you aware of
>> either empty columns or prepacked columns (e.g. metal affinity or ion
>> exchange resins) from other companies?
>> Thanks for your advice
>>
>> Markus
>>
>>
>>
>

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread David Blum 
We have had good luck with columns from Essential Life Solutions (
http://www.essential-life.net/).  They are easy to work with and hold up
under pressure well.

Best,

David

-- 

David L. Blum, Ph.D.

Director, Bioexpression and Fermentation Facility

Department of Biochemistry and Molecular Biology

University of Georgia

120 Green Street room A414A

Athens, GA 30602

http://bff.uga.edu/

Skype: dlblum11

(706) 542-1035 (Office)

(706) 542-1077 (Fax)

On Wed, Feb 15, 2017 at 1:04 PM, Carlos Kikuti  wrote:

> Well GE sells empty columns (XK for low back-pressure, tricorn for higher
> back-pressure) that you can fill with the resin you want. They seem
> expensive but for that kind of things I don't think any option will be real
> 'savings'. For nickel affinity we have been having good results with
> Roche's Complete - it requires less imidazole for binding and elution, and
> at the same time it is compatible with reducing agents and chelators. And
> we still buy the HisTrap FF crude.
>
> On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth <
> christianroth...@gmail.com> wrote:
>
>> Hi Markus,
>>
>> BioRad has columns as well (prepacked and empty), which follow the
>> establiched connector standard. So usable without the need of adapters.
>>
>> Cheers
>>
>> Christian
>>
>> Am 15.02.2017 um 16:57 schrieb Markus Seeliger:
>>
>> Dear all,
>> we are happy users of all that GE offers around their FPLC system, but I
>> am getting a little tired of feeling monopolized. Are any of you aware of
>> either empty columns or prepacked columns (e.g. metal affinity or ion
>> exchange resins) from other companies?
>> Thanks for your advice
>>
>> Markus
>>
>>
>>
>

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-15 Thread Carlos Kikuti 
Well GE sells empty columns (XK for low back-pressure, tricorn for higher
back-pressure) that you can fill with the resin you want. They seem
expensive but for that kind of things I don't think any option will be real
'savings'. For nickel affinity we have been having good results with
Roche's Complete - it requires less imidazole for binding and elution, and
at the same time it is compatible with reducing agents and chelators. And
we still buy the HisTrap FF crude.

On Wed, Feb 15, 2017 at 6:03 PM, Christian Roth 
wrote:

> Hi Markus,
>
> BioRad has columns as well (prepacked and empty), which follow the
> establiched connector standard. So usable without the need of adapters.
>
> Cheers
>
> Christian
>
> Am 15.02.2017 um 16:57 schrieb Markus Seeliger:
>
> Dear all,
> we are happy users of all that GE offers around their FPLC system, but I
> am getting a little tired of feeling monopolized. Are any of you aware of
> either empty columns or prepacked columns (e.g. metal affinity or ion
> exchange resins) from other companies?
> Thanks for your advice
>
> Markus
>
>
>

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-15 Thread Christian Roth 

Hi Markus,

BioRad has columns as well (prepacked and empty), which follow the 
establiched connector standard. So usable without the need of adapters.


Cheers

Christian


Am 15.02.2017 um 16:57 schrieb Markus Seeliger:

Dear all,
we are happy users of all that GE offers around their FPLC system, but 
I am getting a little tired of feeling monopolized. Are any of you 
aware of either empty columns or prepacked columns (e.g. metal 
affinity or ion exchange resins) from other companies?

Thanks for your advice

Markus

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-15 Thread Bonsor, Daniel 
You could try Goldbio.com. I have not used the His-columns from them, but have 
used the Protein A columns and they work as well as the Protein A from GE.

Daniel A Bonsor PhD.
Sundberg Lab
Institute of Human Virology
University of Maryland, Baltimore
725 W Lombard Street N370
Baltimore
Maryland
MD 21201
Tel: (410) 706-7457


From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Markus 
Seeliger
Sent: Wednesday, February 15, 2017 11:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Dear all,
we are happy users of all that GE offers around their FPLC system, but I am 
getting a little tired of feeling monopolized. Are any of you aware of either 
empty columns or prepacked columns (e.g. metal affinity or ion exchange resins) 
from other companies?
Thanks for your advice
Markus