[galaxy-user] Errors when using 'Export to File' option in history pane

2011-07-18 Thread Mattias de Hollander
Hello,

I am experiencing the same issues as described in Bug #588:
https://bitbucket.org/galaxy/galaxy-central/issue/588/errors-when-using-export-to-file-option-in

Are there more people with the same issue or is there a solution
available?

Thanks!

Mattias

-- 
Bioinformatician
Netherlands Institute of Ecology (NIOO-KNAW)
Wageningen, the Netherlands

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[galaxy-user] tailoring backend SGE submission requests to specific applications

2011-07-18 Thread Joseph Hargitai

Hi,

is there a best practices, to replace general SGE requests with requests 
specific to applications -

did anyone actually build such request options to be part of the selectable 
items in the apps menus?


best,
joe

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Re: [galaxy-user] BAM files loading and mandatory grooming

2011-07-18 Thread Jennifer Jackson

Hello L-A,

Would you be able to help with a bit more detail and testing? #2 sounds 
like it may be the issue, but without knowing more right now, I'll 
provided the next troubleshooting steps.


1 - this is in your own install and it is current with the latest -dist 
or are you using -central (which pull?). Did something change between 
the last successful BAM load and the new problem?


2 - without copying them means maybe that you are using a symbolic 
link at your own site? This load is into a Library (this is how to 
move data transferred outside of the Galaxy mechanisms into a history, 
i.e. around a local file system by direct copy or similar). Load into a 
Library first, then copy to history. Instruction in wiki here:

http://wiki.g2.bx.psu.edu/Admin/Data%20Libraries/Uploading%20Library%20Files

3 - or do you mean without copying by using the FTP method set up 
locally, followed by a load into a history?


4 - BAM file metadata (pencil icon - Edit options) looks OK? Or, this 
is the problem?


5 - SAMTools is loaded into your instance? Tools function on other BAM 
files or all? Some simple SAMTools commands (that work with BAM files) 
function line command OK on these (to rule out problem with BAM files 
themselves - missing .bai index could be a problem).


6 - if you start with the same data and convert SAM-BAM within Galaxy, 
does the SAM load and is the resulting BAM file OK?


7 - If you load the BAM file at the public Galaxy web site (using FTP), 
the load is successful and the BAM file once imported into a history 
appears OK? Please share link from this test in case we need to examine. 
(Options - Share or Publish, generate link, email to me and I can share 
with dev team if needed).


Thanks for providing more info or perhaps you will find that one of 
these uncovers the issue,


Best,

Jen
Galaxy team

On 7/18/11 2:05 AM, Louise-Amélie Schmitt wrote:

Hello everyone

Whenever we try to load BAM files without copying them, we get an error
stating the files need grooming, and can't use them at all.

Is it this serious? Would there be a way to bypass that?

Thanks!
L-A
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--
Jennifer Jackson
http://usegalaxy.org/
http://galaxyproject.org/
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Re: [galaxy-user] using files produced by Barcode Splitter

2011-07-18 Thread David K Crossman
Jeremy,

The files need to be groomed using the FastQ Groomer so that 
they will end up in the fastqsanger state.  Then your files will show up in the 
pull-down menus.

David


From: galaxy-user-boun...@lists.bx.psu.edu 
[mailto:galaxy-user-boun...@lists.bx.psu.edu] On Behalf Of Jeremy Coate
Sent: Monday, July 18, 2011 1:44 PM
To: galaxy-user@lists.bx.psu.edu
Subject: [galaxy-user] using files produced by Barcode Splitter

I used the Barcode Splitter tool to split multiplexed RNA-Seq libraries into 
separate files. I would now like to map the reads from each of these fastq 
files to a reference genome. However, the fastq files generated by Barcode 
Splitter don't appear in the Fastq File pull-down menus within the the BWA or 
Bowtie launch pages. I'm probably missing something obvious, but what is the 
trick for making these files available for the mapping tools? Do I need to 
import them into my history somehow?

Thanks!
Jeremy
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