[galaxy-user] Deleting a Galaxy User

2011-11-10 Thread Oren Livne

Dear All,
Is it possible to delete a galaxy user or multiple users with a UI call 
or an API call?

Thanks,
Oren
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[galaxy-user] Question about building a reference genome index using Bowtie

2011-11-10 Thread William Light
I am trying to compare two genetically different strains that I have
sequenced using SOLiD.  I was trying to ask where these two strains are
different, either in terms of deletions or polymorphisms, and one idea I
had was to use Bowtie to create an index from one of these strains and then
to map my reads for the other strain using this index.  Is this a
reasonable strategy?

 - Will Light
   Northwestern University
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[galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Stefanie Ververs

Hi everybody,

I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
I still have some questions:

1) Am I correct that the genetrack indexer seems to be down on the 
Public Galaxy Server? I get a server error, when I start it.
(The Genetrack Browser doesn't work either; although I'm still not quite 
sure whether there are dependencies.)


2) By now, I know one paper on Genetrack - 
http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I 
found the following presentation slides: 
http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. 
Galaxy tells me to cite Blankenberg D, et al. In preparation.


Is there additional information? It would be great to know how exactly 
the peak predictor works, but the slides give only a kind of overview, 
but of course no explaining and no details and the paper isn't that clear.


Thanks,

Steffi/
///
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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg
Hi Steffi,

GeneTrack should be working again on the Main server, thanks for reporting the 
error.

For information on the inner workings of GeneTrack, you should consult the 
paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you can 
additionally contact the GeneTrack author, who I've CC'd here.


Thanks for using Galaxy,

Dan


On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:

 Hi everybody,
 
 I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
 I still have some questions:
 
 1) Am I correct that the genetrack indexer seems to be down on the Public 
 Galaxy Server? I get a server error, when I start it.
 (The Genetrack Browser doesn't work either; although I'm still not quite sure 
 whether there are dependencies.)
 
 2) By now, I know one paper on Genetrack - 
 http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I found 
 the following presentation slides: 
 http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy 
 tells me to cite Blankenberg D, et al. In preparation.
 
 Is there additional information? It would be great to know how exactly the 
 peak predictor works, but the slides give only a kind of overview, but of 
 course no explaining and no details and the paper isn't that clear.
 
 Thanks,
 
 Steffi
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  http://lists.bx.psu.edu/

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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg

 I think in general you should make sure to cite the authors of the
 original publication in addition to Galaxy, the note in Galaxy should
 make this explicit.

Absolutely agree, the citations are arguably the most important part.  The 
GeneTrack paper citation is currently listed in the help for the tools at 
http://usegalaxy.org and in -central. Any place where a citation is missing is 
an error and will be corrected as soon as it is reported.


Thanks for using Galaxy,

Dan

On Nov 10, 2011, at 12:55 PM, Istvan Albert wrote:

 Hello Everyone,
 
 Galaxy tells me to cite Blankenberg D, et al. In preparation.
 
 I think in general you should make sure to cite the authors of the
 original publication in addition to Galaxy, the note in Galaxy should
 make this explicit.
 
 Small tidbits that may be useful. There is a command line version for
 genetrack  with its source code at:
 
 https://github.com/ialbert/chipexo
 
 this is a fork of my son's project while he rotated in a lab, he
 ported a number of tools including GeneTrack to a command line
 interface. Seems to work well but I have not ran it for large genomes.
 
 In the course that I teach the lectures covering ChipSeq analysis: 19,
 20 and 21 cover the usage and principles of GeneTrack (among other
 topics)
 
 http://bcc.bx.psu.edu/courses/597D-2011/index-597D-2011.html
 
 best regards,
 
 Istvan
 
 
 On Thu, Nov 10, 2011 at 12:19 PM, Daniel Blankenberg d...@bx.psu.edu wrote:
 Hi Steffi,
 GeneTrack should be working again on the Main server, thanks for reporting
 the error.
 For information on the inner workings of GeneTrack, you should consult the
 paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you
 can additionally contact the GeneTrack author, who I've CC'd here.
 
 Thanks for using Galaxy,
 Dan
 
 On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:
 
 Hi everybody,
 
 I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
 I still have some questions:
 
 1) Am I correct that the genetrack indexer seems to be down on the Public
 Galaxy Server? I get a server error, when I start it.
 (The Genetrack Browser doesn't work either; although I'm still not quite
 sure whether there are dependencies.)
 
 2) By now, I know one paper on Genetrack -
 http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I
 found the following presentation slides:
 http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy
 tells me to cite Blankenberg D, et al. In preparation.
 
 Is there additional information? It would be great to know how exactly the
 peak predictor works, but the slides give only a kind of overview, but of
 course no explaining and no details and the paper isn't that clear.
 
 Thanks,
 
 Steffi
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  http://lists.bx.psu.edu/
 
 
 
 
 -- 
 Istvan Albert
 Associate Professor, Bioinformatics
 Pennsylvania State University
 http://www.personal.psu.edu/iua1/
 


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at usegalaxy.org.  Please keep all replies on the list by
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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Istvan Albert
Hello Everyone,

 Galaxy tells me to cite Blankenberg D, et al. In preparation.

I think in general you should make sure to cite the authors of the
original publication in addition to Galaxy, the note in Galaxy should
make this explicit.

Small tidbits that may be useful. There is a command line version for
genetrack  with its source code at:

https://github.com/ialbert/chipexo

this is a fork of my son's project while he rotated in a lab, he
ported a number of tools including GeneTrack to a command line
interface. Seems to work well but I have not ran it for large genomes.

In the course that I teach the lectures covering ChipSeq analysis: 19,
20 and 21 cover the usage and principles of GeneTrack (among other
topics)

http://bcc.bx.psu.edu/courses/597D-2011/index-597D-2011.html

best regards,

Istvan


On Thu, Nov 10, 2011 at 12:19 PM, Daniel Blankenberg d...@bx.psu.edu wrote:
 Hi Steffi,
 GeneTrack should be working again on the Main server, thanks for reporting
 the error.
 For information on the inner workings of GeneTrack, you should consult the
 paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you
 can additionally contact the GeneTrack author, who I've CC'd here.

 Thanks for using Galaxy,
 Dan

 On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:

 Hi everybody,

 I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
 I still have some questions:

 1) Am I correct that the genetrack indexer seems to be down on the Public
 Galaxy Server? I get a server error, when I start it.
 (The Genetrack Browser doesn't work either; although I'm still not quite
 sure whether there are dependencies.)

 2) By now, I know one paper on Genetrack -
 http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I
 found the following presentation slides:
 http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy
 tells me to cite Blankenberg D, et al. In preparation.

 Is there additional information? It would be great to know how exactly the
 peak predictor works, but the slides give only a kind of overview, but of
 course no explaining and no details and the paper isn't that clear.

 Thanks,

 Steffi
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:

  http://lists.bx.psu.edu/




-- 
Istvan Albert
Associate Professor, Bioinformatics
Pennsylvania State University
http://www.personal.psu.edu/iua1/

___
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using reply all in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

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[galaxy-user] Question about file formats

2011-11-10 Thread Rena Zheng
Hi,
I uploaded a bed file to Galaxy and did some text manipulations.  I want to 
download the new file as a bed format that I can then open up in excel or a 
text editor.  However, when I save the data, it is a .tabular format that I 
cannot open with these programs.  What should I do?

Thanks,
Rena
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Re: [galaxy-user] Question about file formats

2011-11-10 Thread Peter Cock
On Thu, Nov 10, 2011 at 6:10 PM, Rena Zheng rzh...@mail.med.upenn.edu wrote:
 Hi,
 I uploaded a bed file to Galaxy and did some text manipulations.  I want
 to download the new file as a bed format that I can then open up in excel
 or a text editor.  However, when I save the data, it is a .tabular format that
 I cannot open with these programs.  What should I do?

 Thanks,
 Rena

Try renaming the file to end with .tsv or .txt and Excel or your text editor
should be happy.

Peter

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[galaxy-user] my data files are missing from drop down menus

2011-11-10 Thread Maya Kasowski
Hello,

I uploaded a couple fastq files to Galaxy (Illumina GA2X sequencer).
The files uploaded successfully and are green in the history section.
However, neither file is visible in the drop down menus for any of the
tools. I'd appreciate your help with this.

Thank you,
Maya
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