[galaxy-user] comparing data sets with different coordinates

2011-12-19 Thread dv...@bio.fsu.edu
Hello,

May I use galaxy to do correlation analyses with two continuous
quantitative data sets containing different coordinates (two different
microarray designs)?

How can I create sliding windowed averages for these data sets to
accomplish this?

How can I perform a correlation analysis with a wiggle and a bed (e.g.,
microarray data VS gene density).

Would I need to do a grouping of my microarray data with another
spreadsheet defining the window coordinates?

Thanks,

Dan
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Re: [galaxy-user] Finding Galaxy version

2011-12-19 Thread Nate Coraor
On Dec 13, 2011, at 5:33 AM, Paul-Michael Agapow wrote:

 A perhaps obvious question: how do I work out what version of Galaxy an 
 instance is?
  
 This has come  up a few times because of apparent bugs and different 
 behaviour across various development and production instances. Now if you’re 
 installed straight from the repo, you can use “hg log” for the repo number. 
 But that doesn’t work if it’s been installed in another way, say from a 
 tarball or via another versioning system (we use git to synchronize tool 
 development to a production server).
  
 Simple solution?

Hi Paul,

Since the hg changeset id is the Galaxy version, there's not a great way to 
determine the version without hg.  I'd suggest making a note of the id when you 
fetch the tarball.

--nate

  
 
 Paul Agapow (paul-michael.aga...@hpa.org.uk)
 Bioinformatics, Health Protection Agency
  
 
 
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Re: [galaxy-user] Tophat jobs not starting

2011-12-19 Thread Nate Coraor
On Dec 14, 2011, at 11:19 AM, Magdalena Strzelecka wrote:

 Hi, 
 
 I have submitted some jobs to Tophat, but they have not started since 
 yesterday (Dec 13th); i.e they were in a queue for 12 hrs. I have 
 re-submitted everything again (2 jobs), but the same situation is happening. 
 Is there some issue with Tophat at the moment?

Hi Magdalena,

This was due to a problem with the cluster Galaxy uses to run certain jobs. 
This was resolved early last week, although there may have been a few 
intermittent problems after that time. If tophat still has not run 
successfully, please let us know.

Our sincere apologies for the inconvenience,

Best,
--nate

 Thanks.
 M.
 
 --
 Magdalena Strzelecka, PhD
 
 Heald Lab
 University of California, Berkeley
 Department of Molecular  Cell Biology
 315 Life Sciences Addition # 3200
 Berkeley, CA 94720-3200
 
 phone: (510) 643-5002
 fax: (510) 643-6791
 e-mail: strzele...@berkeley.edu
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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[galaxy-user] Workflows Multiple datasets

2011-12-19 Thread Adhemar
Hi,
I was searching for how to run a workflow for multiple input files and I
was very happy to find it's already implemented as mentioned here:
http://wiki.g2.bx.psu.edu/News%20Briefs/2011_05_20

The problem is that it's not working for me. When I select two files, for
example, it runs the workflow only in the first file. Even if I check 'Send
results to a new history', it only creates a new history called 'test 1'
and not the others. Is my instance somehow misconfigured or it's happening
to others as well?

I'm also looking for a way to add datasets to a library from different
histories. Do I really need to open each history one by one and use 'Import
datasets from your current library'?

Thank you,
Adhemar
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Re: [galaxy-user] Workflows Multiple datasets

2011-12-19 Thread Adhemar
Dannon,
Thanks for the immediate reply.
I just retrieved the latest code from the repository and it's not working

$ hg summary
parent: 6298:b258de1e6cea tip


I also tried 'hg pull -u -r 6368:de03f821784b' but it says:

pulling from https://bitbucket.org/galaxy/galaxy-dist
abort: unknown revision '6368:de03f821784b'!


How or when can I update to the release you mentioned?
Thanks,
-Adhemar


2011/12/19 Dannon Baker dannonba...@me.com

 Adhemar,

 This was a bug that has been fixed as of changeset 6368:de03f821784b,
 which should be available on main.g2.bx.psu.edu in the near future.  If
 you're running a local instance, you'll find this resolved as soon as you
 update to that changeset or higher.

 Unfortunately, there isn't a fast way to copy datasets into a data library
 from multiple histories at once.  I think you might find it faster to use
 the standard Copy Datasets functionality in the history Options menu to
 move everything you'd like into a single new history, and from there move
 it into a library.  This Copy Datasets action allows you to select an
 individual history to copy from, instead of manually changing to the
 history first, so it should save you some time to do it that way.

 -Dannon


 On Dec 19, 2011, at 12:12 PM, Adhemar wrote:

  Hi,
  I was searching for how to run a workflow for multiple input files and I
 was very happy to find it's already implemented as mentioned here:
 http://wiki.g2.bx.psu.edu/News%20Briefs/2011_05_20
 
  The problem is that it's not working for me. When I select two files,
 for example, it runs the workflow only in the first file. Even if I check
 'Send results to a new history', it only creates a new history called 'test
 1' and not the others. Is my instance somehow misconfigured or it's
 happening to others as well?
 
  I'm also looking for a way to add datasets to a library from different
 histories. Do I really need to open each history one by one and use 'Import
 datasets from your current library'?
 
  Thank you,
  Adhemar
 
 
 
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Re: [galaxy-user] Workflows Multiple datasets

2011-12-19 Thread Adhemar
I already tested and it's working like a charm.
Thank you very much Dannon.
-Adhemar

2011/12/19 Dannon Baker dannonba...@me.com

 Ahh, I should have specified, that changeset is in the galaxy-central
 repository and has not been moved to galaxy-dist yet.  I think we're
 testing a few more things, but this should happen in the near future, at
 which point you could simply update from galaxy-dist as usual, or you could
 pull directly from galaxy-central right now if you'd like.

 -Dannon

 On Dec 19, 2011, at 12:50 PM, Adhemar wrote:

  Dannon,
  Thanks for the immediate reply.
  I just retrieved the latest code from the repository and it's not working
 
  $ hg summary
  parent: 6298:b258de1e6cea tip
 
 
  I also tried 'hg pull -u -r 6368:de03f821784b' but it says:
 
  pulling from https://bitbucket.org/galaxy/galaxy-dist
  abort: unknown revision '6368:de03f821784b'!
 
 
  How or when can I update to the release you mentioned?
  Thanks,
  -Adhemar
 
 
  2011/12/19 Dannon Baker dannonba...@me.com
  Adhemar,
 
  This was a bug that has been fixed as of changeset 6368:de03f821784b,
 which should be available on main.g2.bx.psu.edu in the near future.  If
 you're running a local instance, you'll find this resolved as soon as you
 update to that changeset or higher.
 
  Unfortunately, there isn't a fast way to copy datasets into a data
 library from multiple histories at once.  I think you might find it faster
 to use the standard Copy Datasets functionality in the history Options
 menu to move everything you'd like into a single new history, and from
 there move it into a library.  This Copy Datasets action allows you to
 select an individual history to copy from, instead of manually changing to
 the history first, so it should save you some time to do it that way.
 
  -Dannon
 
 
  On Dec 19, 2011, at 12:12 PM, Adhemar wrote:
 
   Hi,
   I was searching for how to run a workflow for multiple input files and
 I was very happy to find it's already implemented as mentioned here:
 http://wiki.g2.bx.psu.edu/News%20Briefs/2011_05_20
  
   The problem is that it's not working for me. When I select two files,
 for example, it runs the workflow only in the first file. Even if I check
 'Send results to a new history', it only creates a new history called 'test
 1' and not the others. Is my instance somehow misconfigured or it's
 happening to others as well?
  
   I'm also looking for a way to add datasets to a library from different
 histories. Do I really need to open each history one by one and use 'Import
 datasets from your current library'?
  
   Thank you,
   Adhemar
  
  
  
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Re: [galaxy-user] CloudMan - The connection has timed out

2011-12-19 Thread mailing list
Hi guys,

It looks like my security group rules didn't get saved.  I'm going to
try again and report back.

I actually asked this question on Biostar too.  I thought my email
didn't make it to the user group.

It probably makes sense to consolidate in one place so I'm continuing
the updates over there:
http://biostar.stackexchange.com/questions/15554/galaxy-cloudman-cant-get-an-instance-started-the-connection-has-timed-out

-Greg



On Sat, Dec 17, 2011 at 3:36 AM, Enis Afgan eaf...@emory.edu wrote:
 Hi Greg,
 Sorry to hear you're having trouble.
 Can you please confirm that you followed the step for creating a security
 group on the wiki and opened all the specified ports?

 Enis

 On Fri, Dec 16, 2011 at 7:58 PM, mailing list margeem...@gmail.com wrote:

 Hi Everyone,

 I'm trying to set up Cloud man.

 I believe I followed the instructions listed here to a T:
 http://wiki.g2.bx.psu.edu/Admin/Cloud

 After launching the instance I even waited 20 minutes before trying to
 access the public DNS
 ec2-xxx-xx-xx-xx.compute-1.amazonaws.com/cloud, but my browser just says:

 The connection has timed out

 The server at ec2-xxx-xx-xx-xx.compute-1.amazonaws.com is taking too
 long to respond.

 Any ideas what I should check?  I'm stuck.  What information would be
 useful?

 Info:

 Zone is us-east-1a
 Type is m1.large
 root devide is ebs
 AMI Name: galaxy-cloudman-2011-03-22
 Region is US East (Virginia)

 I used this format for user data:

 cluster_name: DESIRED CLUSTER NAME
 password: DESIRED Galaxy CloudMan WEB UI PASSWORD
 access_key: YOUR AWS ACCESS KEY
 secret_key: YOUR AWS SECRET KEY

 However this screenshot shows a pipe delimited deal?  Maybe I should try
 that?

 http://userwww.service.emory.edu/~eafgan/content/galaxy_cloud/AWSconsole-4.jpg


 Thanks,

 Greg

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