[galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread David PANG
Hi, Everybody,

I uploaded two complete genome sequences and hope to BLAST homologs from
both genome.

Does anybody know how to do BLAST search on galaxy?

Thanks

David
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Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Björn Grüning
Hi David,

at first you need to predict some genes. In the tool-shed you will find
some gene prediction tools. With these gene you can search against local
BLAST-databases using the NCBI BLAST+ tools, included in Main-Galaxy.

Hope that helps,
Bjoern

 Hi, Everybody,
 
 I uploaded two complete genome sequences and hope to BLAST homologs
 from both genome.
 
 Does anybody know how to do BLAST search on galaxy?
 
 Thanks
 
 David 
 
 
 
 ___
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 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
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-- 
Björn Grüning
Albert-Ludwigs-Universität Freiburg
Institute of Pharmaceutical Sciences
Pharmaceutical Bioinformatics
Hermann-Herder-Strasse 9
D-79104 Freiburg i. Br.

Tel.:  +49 761 203-4872
Fax.:  +49 761 203-97769
E-Mail: bjoern.gruen...@pharmazie.uni-freiburg.de
Web: http://www.pharmaceutical-bioinformatics.org/

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Re: [galaxy-user] user/groups/roles/permissions ???

2012-02-09 Thread Jennifer Jackson

Hello Bernd,

This wiki explains the organization of roles in Galaxy:
http://wiki.g2.bx.psu.edu/Learn/Security%20Features

And if you need help with the Admin UI, this wiki can help:
http://wiki.g2.bx.psu.edu/Admin/Interface

Most administration help located in the wiki organized under this section:
http://wiki.g2.bx.psu.edu/Admin

The galaxy-...@bx.psu.edu mailing list is a good choice
for local instance help.
http://wiki.g2.bx.psu.edu/Mailing%20Lists
http://wiki.g2.bx.psu.edu/Mailing%20Lists#Subscribing_and_Unsubscribing

Prior Q/A can also be searched with:
http://galaxy.psu.edu/search/mailinglists

Hopefully this helps,

Best,

Jen
Galaxy team

On 2/8/12 4:13 AM, Bernd Jagla wrote:

Hi,

I am relatively new to Galaxy and couldn't find an explaination on how
you envision the concepts of users/groups/roles etc...

I am managing a Galaxy instance and have different groups of biologist
(users), who should all are allowed to see the data from that group.
Then I have a group of users who are not super users, but still should
be able to see data from different groups of biologistl; they should
also be able to upload data, other should not...

I would like to make sure that my understanding of groups and users is
the same as that of Galaxy and also would like to find explaination and
possibly example of how to use roles and potentially other tools.

Could you please point me to where in the documentation this explained?

Kind regards,

Bernd

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--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
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Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Jennifer Jackson

Hi David,

It sounds like you are using the public Galaxy server at 
http://usegalaxy.org (http://main.g2.bx.psu.edu)? A BLAST service is not 
available on the public instance. (Megablast is available, but for NGS 
query data versus a specific set of target native genomes).


Bjoern is correct that you will need to obtain a Galaxy BLAST wrapper 
from the Tool Shed.

http://wiki.g2.bx.psu.edu/Tool%20Shed
Tools Shed entries can be searched for with:
http://galaxy.psu.edu/search/getgalaxy
These would be installed into a local or cloud version of Galaxy and set 
up using the following instructions:

http://getgalaxy.org

The galaxy-...@bx.psu.edu mailing list is a good choice if you need 
help/advice with set up:

http://wiki.g2.bx.psu.edu/Support#Mailing_Lists
Prior Q/A can also be searched with:
http://galaxy.psu.edu/search/mailinglists

Best wishes for your project,

Jen
Galaxy team

On 2/9/12 9:47 AM, David PANG wrote:

Hi, Everybody,

I uploaded two complete genome sequences and hope to BLAST homologs from
both genome.

Does anybody know how to do BLAST search on galaxy?

Thanks

David





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--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
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Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Peter Cock
On Thursday, February 9, 2012, Jennifer Jackson j...@bx.psu.edu wrote:
 Hi David,

 It sounds like you are using the public Galaxy server at
 http://usegalaxy.org (http://main.g2.bx.psu.edu)? A
 BLAST service is not available on the public instance.
 (Megablast is available, but for NGS query data versus
 a specific set of target native genomes).

 Bjoern is correct that you will need to obtain a Galaxy
 BLAST wrapper from the Tool Shed.
 http://wiki.g2.bx.psu.edu/Tool%20Shed
 Tools Shed entries can be searched for with:
 http://galaxy.psu.edu/search/getgalaxy
 These would be installed into a local or cloud version
 of Galaxy and set up using the following instructions:
 http://getgalaxy.org


Hi Jen,

The NCBI BLAST wrappers used to be included as standard tools
when doing a local Galaxy server installation - and were not in
the ToolShed. Has that changed?

Peter
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Re: [galaxy-user] How to do BLAST on Galaxy sever?

2012-02-09 Thread Jennifer Jackson

Hi Peter,

It looks to me like there are versions both, with the Tool Shed version 
having the more recent date stamp. But if those extra functions are not 
needed, then yes, you are correct, the version included in the 
distribution would be enough.


Please send corrections if I have any of this wrong, so that David gets 
the best information,


Thanks Peter!

Jen
Galaxy team

On 2/9/12 1:50 PM, Peter Cock wrote:



On Thursday, February 9, 2012, Jennifer Jackson j...@bx.psu.edu
mailto:j...@bx.psu.edu wrote:
  Hi David,
 
  It sounds like you are using the public Galaxy server at
  http://usegalaxy.org (http://main.g2.bx.psu.edu)? A
  BLAST service is not available on the public instance.
  (Megablast is available, but for NGS query data versus
  a specific set of target native genomes).
 
  Bjoern is correct that you will need to obtain a Galaxy
  BLAST wrapper from the Tool Shed.
  http://wiki.g2.bx.psu.edu/Tool%20Shed
  Tools Shed entries can be searched for with:
  http://galaxy.psu.edu/search/getgalaxy
  These would be installed into a local or cloud version
  of Galaxy and set up using the following instructions:
  http://getgalaxy.org
 

Hi Jen,

The NCBI BLAST wrappers used to be included as standard tools
when doing a local Galaxy server installation - and were not in
the ToolShed. Has that changed?

Peter


--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
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Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
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local Galaxy instances and the Galaxy source code, please
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 http://lists.bx.psu.edu/listinfo/galaxy-dev

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