Re: [Histonet] RE: Cornflaking artifact
Is this a tape coverslipper? If so, you do have minute traces of water carryover to your xylene. If there is even the hint of pink in you last dehydrant before xylene, you will get that cornflake artifact. Acetone wont help, because water still be present. Increase number of absolute alcohol before xylene, and check often for eosin carryover. This fixed our problem. Jackie O' Sent from my iPhone On Mar 12, 2014, at 21:59, Sharon Scalise sscal...@beaumont.edu wrote: I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cornflaking artifact
You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MUM-1
Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the usual adjustments and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cornflaking artifact
We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Cornflaking artifact
We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca To: 'Laurie Colbert' lcolb...@pathmdlabs.com, Sharon Scalise sscal...@beaumont.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 03/13/2014 11:43 AM Subject:[Histonet] RE: Cornflaking artifact Sent by:histonet-boun...@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more.
[Histonet] RE: Cornflaking artifact
Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metz...@aruplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Thursday, March 13, 2014 9:42 AM To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws,
[Histonet] RE: MUM-1
This is our protocol for the Ultra; maybe it will help. 64 CC1, 32 incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4. This is with UltraView DAB detection. We use tonsil as well however we validated with HD, LN, GI, tonsil, etc. Good luck! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Thursday, March 13, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM-1 Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the usual adjustments and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cornflaking artifact
Long time and high volume user of the Sakura Tape. Check the Xylene drops. We coverslip thousands of slides daily and have seen no isssue with the tape lots. Our experience shows some cornflaking when the slide does not receive enough xylens to wet the slide properly. William DeSalvo, BS HTL(ASCP) From: kenneth.metz...@aruplab.com To: sheila.herring...@interiorhealth.ca; lcolb...@pathmdlabs.com; sscal...@beaumont.edu; histonet@lists.utsouthwestern.edu Date: Thu, 13 Mar 2014 16:13:24 + CC: Subject: [Histonet] RE: Cornflaking artifact Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metz...@aruplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Thursday, March 13, 2014 9:42 AM To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cornflaking artifact
We have changed lots of tape and still are having this artifact. Lisa LaVigne CT, MB (ASCP) Pathology Manager St. Peter's Health Partners 315 S. Manning Blvd. Albany, NY 12208 Phone: 518-525-5274 Fax: 518-525-6750 Email: lisa.lavi...@sphp.commailto:lisa.lavi...@sphp.com From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth [kenneth.metz...@aruplab.com] Sent: Thursday, March 13, 2014 12:13 PM To: HERRINGTON, SHEILA; 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metz...@aruplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Thursday, March 13, 2014 9:42 AM To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet
[Histonet] New Jersey Meeting
I just wanted to send a reminder that this Friday, March 15th is the registration deadline for the NJSH Spring Meeting planned for April 5th in Mount Laurel, NJ. There will be four 90 minute seminars entitled Respiratory Histopathology, Pathology of the Urinary Tract, Animal Model Development to Aid in the Understanding of Human Atherosclerosis, and Predictive Testing in Non-small Cell Lung Cancer: Testing Protocols, Specimen Requirements and Near Future Techniques, Notably Next Generation Sequencing. The cost is ONLY $90 for the day and you will receive 6 CEUs for attending! For more information and to register on-line, please visit our website http://njsh.org/njsh/ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC without wash buffer
In a continuing effort to limit the volume of reagent used in each step of a manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and without barriers. The results were not stellar with the barrier slides - the reagent still escapes. We dewax with hot detergent (may be a contributing factor) and the Tween 20 in the TBST definitely alters the hydrophobicity of the barrier, an effect that is not reversed with a water wash. Consequently, I have resorted to omitting the buffer wash steps and using distilled water only. The slide surface remains water repellent and the added IHC reagents form a pool over the tissue sections. The detection method is a polymer-HRP and there is no increase in background staining in the tissue or on the slide surface. I am assuming that the IHC reagents are prepared in the optimum suspension liquid and a buffer is not required. So, I am interested in hearing from anyone why this is a bad idea. Thanks, Tresa [X] [X] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] request for surplus equipment
Dear Histonet, A colleague in Manicaland Province, Mutare, Zimbabwe is in need of a thermocycler if anyone has an old one that they would be able to donate. The colleague is the Coordinator for Laboratory Training at Africa University.She and the university work to provide opportunities for students from all over Africa to learn clinical laboratory science skills. Please contact me off list if you can help and I will arrange shipping and put you in contact with the university! Thank you, -- Sarah Holmes Laboratory Manager Laboratory for Kidney Pathology, Inc. 1916 Patterson St, Suite 501 Nashville, TN 37203 615 321 5729 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Cornflaking artifact
We had this issue when we were using the tape from Mercedes Medical and went back to Sakura tape and it went away. It could also be associated with humidity. STP TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Cornflaking
Why doesn't someone call Kellogg's ? On Mar 13, 2014, at 12:10 PM, Beth Cox bethc...@gmail.com wrote: Sharon other Histonetters, Cornflaking is literally microscopic air trapped under the coverslip. It doesn't have anything to do with poor dehydration and trapped water. Thus it can be caused by partial drying out before the coverslipping. The things you need to look at to eradicate the problem is: 1. Keep the slides wet before coverslipping (obviously) 2. Consider increasing the amount of xylene deposited on the slide for tape coverslippers. 3. For glass coverslippers, consider increasing the xylene and increasing or changing the mounting media. Cornflaking tends to happen more on slides/sections with rough topography on the section (the more rough it is, the more nooks crannies to trap the air). So anything that would give the section a more rough surface would increase the tendency to cornflake; such as: section lifting. section thickness, even chatter in the sections. Think about things that would affect the section - for example, are you using a different brand of blade on the microtome? Hope this helps, Beth Cox, HTL/SCT(ASCP)QIHC Message: 15 Date: Thu, 13 Mar 2014 11:59:06 -0400 From: sris...@mail.holyname.org Subject: Re: [Histonet] RE: Cornflaking artifact To: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,Sharon Scalise sscal...@beaumont.edu, histonet-boun...@lists.utsouthwestern.edu, 'Laurie Colbert' lcolb...@pathmdlabs.com Message-ID: of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org Content-Type: text/plain; charset=US-ASCII We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca To: 'Laurie Colbert' lcolb...@pathmdlabs.com, Sharon Scalise sscal...@beaumont.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 03/13/2014 11:43 AM Subject:[Histonet] RE: Cornflaking artifact Sent by:histonet-boun...@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.eduhistonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.eduhistonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using
[Histonet] Re: Cornflaking
Sharon other Histonetters, Cornflaking is literally microscopic air trapped under the coverslip. It doesn't have anything to do with poor dehydration and trapped water. Thus it can be caused by partial drying out before the coverslipping. The things you need to look at to eradicate the problem is: 1. Keep the slides wet before coverslipping (obviously) 2. Consider increasing the amount of xylene deposited on the slide for tape coverslippers. 3. For glass coverslippers, consider increasing the xylene and increasing or changing the mounting media. Cornflaking tends to happen more on slides/sections with rough topography on the section (the more rough it is, the more nooks crannies to trap the air). So anything that would give the section a more rough surface would increase the tendency to cornflake; such as: section lifting. section thickness, even chatter in the sections. Think about things that would affect the section - for example, are you using a different brand of blade on the microtome? Hope this helps, Beth Cox, HTL/SCT(ASCP)QIHC Message: 15 Date: Thu, 13 Mar 2014 11:59:06 -0400 From: sris...@mail.holyname.org Subject: Re: [Histonet] RE: Cornflaking artifact To: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,Sharon Scalise sscal...@beaumont.edu, histonet-boun...@lists.utsouthwestern.edu, 'Laurie Colbert' lcolb...@pathmdlabs.com Message-ID: of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org Content-Type: text/plain; charset=US-ASCII We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca To: 'Laurie Colbert' lcolb...@pathmdlabs.com, Sharon Scalise sscal...@beaumont.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 03/13/2014 11:43 AM Subject:[Histonet] RE: Cornflaking artifact Sent by:histonet-boun...@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.eduhistonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.eduhistonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our cornflaking started at the same
[Histonet] GI Biopsies
Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Cornflaking
We are all putting out what worked for us. You should try some of these suggestions and see what works for you. The suggestion for improving poor dehydration was suggested by technical support from Sakura. Cornflaking was irritating so many of our pathologist, we had to get technical support from Sakura to see how this could be resolved. In OUR hospital changing the tape worked!!! Good Luck.!!! Mala Nirmala Srishan Holy Name Medical Center. From: joe joe powderhoun...@hotmail.com To: Beth Cox bethc...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 03/13/2014 02:20 PM Subject:Re: [Histonet] Re: Cornflaking Sent by:histonet-boun...@lists.utsouthwestern.edu Why doesn't someone call Kellogg's ? On Mar 13, 2014, at 12:10 PM, Beth Cox bethc...@gmail.com wrote: Sharon other Histonetters, Cornflaking is literally microscopic air trapped under the coverslip. It doesn't have anything to do with poor dehydration and trapped water. Thus it can be caused by partial drying out before the coverslipping. The things you need to look at to eradicate the problem is: 1. Keep the slides wet before coverslipping (obviously) 2. Consider increasing the amount of xylene deposited on the slide for tape coverslippers. 3. For glass coverslippers, consider increasing the xylene and increasing or changing the mounting media. Cornflaking tends to happen more on slides/sections with rough topography on the section (the more rough it is, the more nooks crannies to trap the air). So anything that would give the section a more rough surface would increase the tendency to cornflake; such as: section lifting. section thickness, even chatter in the sections. Think about things that would affect the section - for example, are you using a different brand of blade on the microtome? Hope this helps, Beth Cox, HTL/SCT(ASCP)QIHC Message: 15 Date: Thu, 13 Mar 2014 11:59:06 -0400 From: sris...@mail.holyname.org Subject: Re: [Histonet] RE: Cornflaking artifact To: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,Sharon Scalise sscal...@beaumont.edu, histonet-boun...@lists.utsouthwestern.edu, 'Laurie Colbert' lcolb...@pathmdlabs.com Message-ID: of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org Content-Type: text/plain; charset=US-ASCII We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca To: 'Laurie Colbert' lcolb...@pathmdlabs.com, Sharon Scalise sscal...@beaumont.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 03/13/2014 11:43 AM Subject:[Histonet] RE: Cornflaking artifact Sent by:histonet-boun...@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with cornflaking (tiny, brown dry
RE: [Histonet] GI Biopsies
We do 3 levels on one slide. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of imhype...@aol.com Sent: Thursday, March 13, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GI Biopsies
We actually cut 2 slides with 4 levels 2 on each slide. Out pathologists were unwilling to only cut one slide ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cornflakes
As a long time user of film (though sadly, no longer) we used to see what sounds like this artifact when the Xylene dispenser was not dispensing enough xylene and the slides were not wet enough. It drove us crazy until we realized that through the years, the xylene dispensing knob had become very loose and would readjust itself at the slightest touch or vibration. We liked our Xylene to be dispensing rapidly, just short of a steady stream, so constantly checked it. Also, if your slides are not completely dehydrated before clearing in Xylene, you are just asking for problems, if not now, then years down the road when the tape will pull off the slide, taking your tissue with it. Been there, done that. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 10 Date: Thu, 13 Mar 2014 02:59:38 + From: Sharon Scalise sscal...@beaumont.edu Subject: [Histonet] RE: Cornflaking artifact To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu I am looking for help with cornflaking (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: MUM-1
I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it stains great on tonsil and kidney tubules. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: cda...@che-east.org; histonet@lists.utsouthwestern.edu Date: Thu, 13 Mar 2014 16:19:11 + CC: Subject: [Histonet] RE: MUM-1 This is our protocol for the Ultra; maybe it will help. 64 CC1, 32 incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4. This is with UltraView DAB detection. We use tonsil as well however we validated with HD, LN, GI, tonsil, etc. Good luck! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Thursday, March 13, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM-1 Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the usual adjustments and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GI Biopsies
3 levels - 1 slide -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of imhype...@aol.com Sent: Thursday, March 13, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH
are you looking for the stats in the notes for ANP. 22970 (2012) ? overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower average 20-35% in post menopausal) , lower in well differentiated tumors...etc. I sure CAP can also send or repeat them to you if you prefer to call them. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 13 Mar 2014 14:06:23 -0400 From: tbr...@holyredeemer.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH Try calling CAP. They provided the benchmarks we use for our annual statistics for ER/PR. I don't have the recent CAP checklist handy, but on the 9.25.2012 checklist, the benchmarks for ER/PR are published in the notes section of the question. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 3 Date: Wed, 12 Mar 2014 18:40:53 + From: O'neil, Beth one...@wvuhealthcare.com Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. Where would one even find published benchmarks? Thank you Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals one...@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) ** - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH
sorry , saw the Predictive, missed FISH - getting ready to do this myself. I would just call CAP, if I get to do that soon I will send along what information they supply. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joellewea...@hotmail.com To: tbr...@holyredeemer.com; histonet@lists.utsouthwestern.edu Date: Thu, 13 Mar 2014 18:44:37 + Subject: RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH CC: are you looking for the stats in the notes for ANP. 22970 (2012) ? overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower average 20-35% in post menopausal) , lower in well differentiated tumors...etc. I sure CAP can also send or repeat them to you if you prefer to call them. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Thu, 13 Mar 2014 14:06:23 -0400 From: tbr...@holyredeemer.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH Try calling CAP. They provided the benchmarks we use for our annual statistics for ER/PR. I don't have the recent CAP checklist handy, but on the 9.25.2012 checklist, the benchmarks for ER/PR are published in the notes section of the question. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 3 Date: Wed, 12 Mar 2014 18:40:53 + From: O'neil, Beth one...@wvuhealthcare.com Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. Where would one even find published benchmarks? Thank you Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals one...@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) ** - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GI Biopsies
Three levels one slide for all biopsies. Colon polyps one section. Sent from my iPhone On Mar 13, 2014, at 11:29 AM, imhype...@aol.com wrote: Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Opening Dallas
IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1 - 2 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 2:00 p.m. to 10:30 p.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE M/F/Disabled/Veteran Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibil...@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica CM3600 Cryomacrotome XP
Hello Histonetters, Is anyone currently or in the past had experience operating the Leica CM3600 Cryomacrotome XP? I am looking for input on using this instrument. Thanks, Tina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
