[Histonet] Paraffin times; paraffin embedding station

[Flynn, Evelyn via Histonet]

Hello,


   Not to be the devil's advocate, but in our research lab we routinely put 
bone specimens in a vacuum oven at 60 degrees overnight or several days to 
promote better infiltration after processing.  Somewhere I was taught that 
specimens are not damaged in the paraffin bath as long as the temperature is at 
60 degrees, not higher.


I would like to thank those Histonetters who suggested a Sakura Tissue-Tek 
embedding station when we

were purchasing such an item.  We have been very satisfied with the unit, and 
it looks very nice in the lab.


Evelyn Flynn

Boston Children's Hospital
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Re: [Histonet] Send-Outs

[Cristi Rigazio via Histonet]

Thanks everyone for the responses!  And just to make sure...I was joking with 
the Amazon reference, lol!  But I do aim high!

Sent from my iPhone

> On May 16, 2017, at 2:37 PM, Dragoo, Michelle  wrote:
> 
> We are the same as Tim. Typically we try to get things out within 24 hours, 
> that said, it depends on how old the case is, does it need recuts etc. etc. I 
> think your current time of 48 hours sounds quite reasonable. 
> 
> Michelle R. Dragoo, MBA, HT (ASCP)
> Supervisor, Histology 
> Northwestern Medicine Central Dupage Hospital
> 25 North Winfield Road, Winfield IL 60190
> O: 630.933.6748
> michelle.dra...@nm.org
> 
> 
> 
> 
> 
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
> Sent: Tuesday, May 16, 2017 10:53 AM
> To: Cristi Rigazio
> Cc: Histonet
> Subject: Re: [Histonet] Send-Outs
> 
> Really? You want to compare your lab to Amazon? If you have stock on-hand, 
> located in one place, have robots to retrieve it, people who do nothing but 
> pack boxes and trucks standing by to take any order at any time of day, then 
> you are in the league of Amazon and can receive an order and send it out 
> within an hour.
> 
> However most of us first need to find the material to send out - slides? 
> where are they? Pathologist, resident? waiting to be filed? Removed from file 
> by someone else? Can't find anything in THAT office...Same with blocks - not 
> in file, is there a card telling who took it? Maybe for recuts, maybe for 
> research, then back into the batch to be filed. Look 5 different places. 
> Frozen tissue is usually easier but, whoops...already sent some out for 
> another requested test...
> 
> Anyway, that is what my experience is!
> 
> We don't have any set time requirement. It could be an hour or a few days 
> depending on if can easily find the material they want to send. Usually it is 
> within a day.
> 
> 
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
> 
> 
> 
> -Original Message-
> From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Monday, May 15, 2017 10:55 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Send-Outs
> 
> Hi Histoland!
> 
> We have been reviewing our send out procedures and I have been tasked with 
> researching what TAT's other institutions define.  Currently, we allow 48 
> hours for the preparation of "orders" to the time sent out.  Is this 
> reasonable?  Do other facilities allow more or less time?
> 
> I feel like Amazon can get something to my door step in a matter of hours, so 
> I must be missing something obvious in our steps that could be simplified, 
> but I can't put my finger on it.  Any feedback on what others are doing is 
> greatly appreciated.
> 
> Sincerely,
> Cristi
> ___
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> 
> 
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> unlawful. If you are not the addressee, please promptly delete this message 
> and notify the sender of the delivery error by e-mail.
> 

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Re: [Histonet] A Question About Paraffin Times

[Weems, Joyce K. via Histonet]

And that should say MUCH better..

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email. 


-Original Message-
From: Weems, Joyce K. via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 16, 2017 3:15 PM
To: P Sicurello 
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] A Question About Paraffin Times

Must better to remove them on Sat than to leave in the hot paraffin. We do this 
all the time so we can meet the CAP time in formalin for the breasts.

Good luck! j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet 
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in 
molten paraffin (62 degrees C)  is bad practice, and causes them to get 
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



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Re: [Histonet] A Question About Paraffin Times

[Cindy Bird via Histonet]

I agree?

Sent from my iPhone

> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet 
>  wrote:
> 
> Hello Paula,
> So why must the cassettes be removed on Saturday morning if you aren't 
> embedding until Monday morning?
> Don't you have a "delay start" feature on your processor? Neither option A or 
> B seem like a good thing for the tissue!
> We time our processor to finish at 5 AM Monday when the first Histotech 
> arrives for embedding.
> The cassettes remain in formalin until the processor starts up on Sunday 
> evening.
> 
> Regards,
> 
> 
> Shannon H. Logan  B.S., HTL (ASCP)
> Pathology Department
> 
> Bellin Health Memorial Hospital
> 744 South Webster Avenue
> Green Bay, WI 54305-3400
> 920-433-3653  X3727
> 
> 
> 
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, May 16, 2017 10:34 AM
> To: HistoNet
> Subject: [Histonet] A Question About Paraffin Times
> 
> Good Morning Listers,
> 
> I am asking the collective wisdom of the Histonet this question:
> 
> 
> Is it better to remove baskets from the processor on Saturday morning and:
> 
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
> 
> 
> I am of the opinion that leaving the samples (not fatty, like breast cores)
> in molten paraffin (62 degrees C) is bad practice, and causes them to get
> "crunchy", among other things.
> 
> What do you think?
> 
> Thank in advance.
> 
> Sincerely,
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 200 Arbor Drive
> 
> San Diego, CA 92103
> 
> (P): 619-543-2872 <#>
> 
> 
> 
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> ___
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Re: [Histonet] A Question About Paraffin Times

[Seeley, Heather via Histonet]

We do a delay protocol as well. The tissue samples will sit in formalin and 
start processing Sunday night for a normal amount of time to come off Monday 
morning. If this is not a possibility, it would be better to let them harden in 
the paraffin than to leave them in the hot paraffin, as this will lead to the 
tissue becoming brittle and very difficult to section. 

HEATHER SEELEY, HT(ASCP)
Histotech
803-985-4676 OFFICE
803-327-7598 FAX



From: P Sicurello [pat...@gmail.com]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
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retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
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Re: [Histonet] A Question About Paraffin Times

[Weems, Joyce K. via Histonet]

Must better to remove them on Sat than to leave in the hot paraffin. We do this 
all the time so we can meet the CAP time in formalin for the breasts.

Good luck! j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
770-380-8099 Cell
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet 
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in 
molten paraffin (62 degrees C)  is bad practice, and causes them to get 
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
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dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.
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Re: [Histonet] A Question About Paraffin Times

[Garcia, Lori, M.Sc. via Histonet]

Hi Paula,

I am in agreement with everyone else about putting the processor on a delay so 
it will finish when needed. However, if that is not possible, it is perfectly 
fine for the blocks to harden at room temp, not freezing, and then be warmed up 
again for embedding. This has come up in my research lab, and we haven't had 
any problems.

Regards,
Lori

This message has been marked as Medtronic Controlled
-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:30 AM
To: Logan, Shannon 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] A Question About Paraffin Times

Hi Shannon and Everyone Else,

Usually the samples are placed on a weekend delay.  This time, however, the 
breast cases were sitting in formalin since Thursday and the run had to end on 
Saturday due to the CAP 72 hour rule for breast and HER2 testing.

The problem is, the person in charge of Histology not a Histologist.  The 
person in charge was told by several experienced histotechs that letting the 
samples sit in molten paraffin is not a good thing to do.  They were told that 
it was routine (which it isn't) to let them sit in hot wax for days.

I just need information that states that sitting in paraffin for any excessive 
length of time is bad.  I found it mentioned in Sheehan and Hrapcek's "Theory 
and Practice of Histotechnology".

I can't find my Carson.  Does she mention excessive times in paraffin?

If so, please let me know.

Thank you for all the assistance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
confidential and/or privileged material.  Any review, retransmission, 
dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.

On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon 
wrote:

> Hello Paula,
>
> So why must the cassettes be removed on Saturday morning if you aren’t
> embedding until Monday morning?
>
> Don’t you have a “delay start” feature on your processor? Neither
> option A or B seem like a good thing for the tissue!
>
>  We time our processor to finish at 5 AM Monday when the first
> Histotech arrives for embedding.
>
> The cassettes remain in formalin until the processor starts up on
> Sunday evening.
>
>
>
> Regards,
>
>
>
>
>
> Shannon H. Logan  B.S., HTL (ASCP)
>
> Pathology Department
>
>
>
> Bellin Health Memorial Hospital
>
> 744 South Webster Avenue
>
> Green Bay, WI 54305-3400
>
> 920-433-3653  X3727 <(920)%20433-3653>
>
>
>
>
>
>
>
> *From:* P Sicurello via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
>
> *Sent:* Tuesday, May 16, 2017 10:34 AM
> *To:* HistoNet
> *Subject:* [Histonet] A Question About Paraffin Times
>
>
>
> Good Morning Listers,
>
> I am asking the collective wisdom of the Histonet this question:
>
>
> Is it better to remove baskets from the processor on Saturday morning and:
>
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
>
>
> I am of the opinion that leaving the samples (not fatty, like breast
> cores) in molten paraffin (62 degrees C) is bad practice, and causes
> them to get "crunchy", among other things.
>
> What do you think?
>
> Thank in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <(619)%20543-2872> <#>
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail
> is intended only for the person or entity to which it is addressed and
> may contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action
> in reliance upon this information by persons or entities other than
> the intended recipient is prohibited. If you received this e-mail in
> error, please contact the sender and delete the material from any computer.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] Send-Outs

[Dragoo, Michelle via Histonet]

We are the same as Tim. Typically we try to get things out within 24 hours, 
that said, it depends on how old the case is, does it need recuts etc. etc. I 
think your current time of 48 hours sounds quite reasonable. 

Michelle R. Dragoo, MBA, HT (ASCP)
Supervisor, Histology 
Northwestern Medicine Central Dupage Hospital
25 North Winfield Road, Winfield IL 60190
O: 630.933.6748
michelle.dra...@nm.org





-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
Sent: Tuesday, May 16, 2017 10:53 AM
To: Cristi Rigazio
Cc: Histonet
Subject: Re: [Histonet] Send-Outs

Really? You want to compare your lab to Amazon? If you have stock on-hand, 
located in one place, have robots to retrieve it, people who do nothing but 
pack boxes and trucks standing by to take any order at any time of day, then 
you are in the league of Amazon and can receive an order and send it out within 
an hour.

However most of us first need to find the material to send out - slides? where 
are they? Pathologist, resident? waiting to be filed? Removed from file by 
someone else? Can't find anything in THAT office...Same with blocks - not in 
file, is there a card telling who took it? Maybe for recuts, maybe for 
research, then back into the batch to be filed. Look 5 different places. Frozen 
tissue is usually easier but, whoops...already sent some out for another 
requested test...

Anyway, that is what my experience is!

We don't have any set time requirement. It could be an hour or a few days 
depending on if can easily find the material they want to send. Usually it is 
within a day.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Send-Outs

Hi Histoland!

We have been reviewing our send out procedures and I have been tasked with 
researching what TAT's other institutions define.  Currently, we allow 48 hours 
for the preparation of "orders" to the time sent out.  Is this reasonable?  Do 
other facilities allow more or less time?

I feel like Amazon can get something to my door step in a matter of hours, so I 
must be missing something obvious in our steps that could be simplified, but I 
can't put my finger on it.  Any feedback on what others are doing is greatly 
appreciated.

Sincerely,
Cristi
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laws. Unauthorized forwarding, printing, copying, distribution, or use of such 
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Re: [Histonet] A Question About Paraffin Times

[P Sicurello via Histonet]

Hi Shannon and Everyone Else,

Usually the samples are placed on a weekend delay.  This time, however,
the breast cases were sitting in formalin since Thursday and the run had to
end on Saturday due to the CAP 72 hour rule for breast and HER2 testing.

The problem is, the person in charge of Histology not a Histologist.  The
person in charge was told by several experienced histotechs that letting
the samples sit in molten paraffin is not a good thing to do.  They were
told that it was routine (which it isn't) to let them sit in hot wax for
days.

I just need information that states that sitting in paraffin for any
excessive length of time is bad.  I found it mentioned in Sheehan and
Hrapcek's "Theory and Practice of Histotechnology".

I can't find my Carson.  Does she mention excessive times in paraffin?

If so, please let me know.

Thank you for all the assistance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.

On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon 
wrote:

> Hello Paula,
>
> So why must the cassettes be removed on Saturday morning if you aren’t
> embedding until Monday morning?
>
> Don’t you have a “delay start” feature on your processor? Neither option A
> or B seem like a good thing for the tissue!
>
>  We time our processor to finish at 5 AM Monday when the first Histotech
> arrives for embedding.
>
> The cassettes remain in formalin until the processor starts up on Sunday
> evening.
>
>
>
> Regards,
>
>
>
>
>
> Shannon H. Logan  B.S., HTL (ASCP)
>
> Pathology Department
>
>
>
> Bellin Health Memorial Hospital
>
> 744 South Webster Avenue
>
> Green Bay, WI 54305-3400
>
> 920-433-3653  X3727 <(920)%20433-3653>
>
>
>
>
>
>
>
> *From:* P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>
> *Sent:* Tuesday, May 16, 2017 10:34 AM
> *To:* HistoNet
> *Subject:* [Histonet] A Question About Paraffin Times
>
>
>
> Good Morning Listers,
>
> I am asking the collective wisdom of the Histonet this question:
>
>
> Is it better to remove baskets from the processor on Saturday morning and:
>
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
>
>
> I am of the opinion that leaving the samples (not fatty, like breast cores)
> in molten paraffin (62 degrees C) is bad practice, and causes them to get
> "crunchy", among other things.
>
> What do you think?
>
> Thank in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <(619)%20543-2872> <#>
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
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Re: [Histonet] A Question About Paraffin Times

[Logan, Shannon via Histonet]

Hello Paula,
So why must the cassettes be removed on Saturday morning if you aren't 
embedding until Monday morning?
Don't you have a "delay start" feature on your processor? Neither option A or B 
seem like a good thing for the tissue!
 We time our processor to finish at 5 AM Monday when the first Histotech 
arrives for embedding.
The cassettes remain in formalin until the processor starts up on Sunday 
evening.

Regards,


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727



From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 10:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A. Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B. Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C) is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
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retransmission, dissemination or other use of or taking of any action in
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intended recipient is prohibited. If you received this e-mail in error,
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Re: [Histonet] Send-outs, cassette printers, holding cassettes, and cassette clamps

[Terri Braud via Histonet]

   1. Send-Outs (Cristi Rigazio)
Our TAT for send-outs is 1 working day (M-F). The office staff here use the 
"Slide/block Send Out" function in CoPath to track all material moving in and 
out of Anatomic Pathology and it really speed up retrieval of case material 
   
   3. Cassette printers - multi color cassettes and network ready-  (Cheryl)
Even though they are big, we still love our Leica Printers.  They are such 
workhorses and they were so easy to interface.

   5. A Question About Paraffin Times (P Sicurello)
I agree that if possible, the delay should be in formalin, but if you are 
running into a problem where that might exceed fixation times for a breast 
specimen there are 2 good alternatives.  The first one is as you stated, to 
just pull them out of paraffin and let them chill, OR you can program a "hold" 
in 70% alcohol to avoid formalin fixation beyond 72 hours, and have the process 
finish at the usual time.  Works like a charm.  Feel free to contact me for 
details. I would never leave cassettes to "cook" in hot paraffin.

   6. Extra Large Cassette Clamps for Microtomes (Sandra Cheasty)
Only use one microtome, even if you are swapping out.  Just my personal, 
unscientific opinion, but the less one disturbs the clamp/knife holder 
assembly, the better.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor, Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal


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Re: [Histonet] cassette printers - multi color cassettes and network ready-

[Cheryl via Histonet]

Thanks to those sending me printer information.  There were a couple we didn't 
know existed ! Cheryl    



   


-Original Message-
From: Cheryl via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 12:57 PM
To: Histonet
Subject: [Histonet] cassette printers - multi color cassettes and network ready-

What are you using for your cassette printer?   
We need 3-5 colors of cassettes, and it has to network so we can have multiple 
users.  We print two lines (number and name) with hopes to go to full bar 
coding in the near future. Looking for any/all input - vendors welcome, too. 
Cheryl Kerry, HT(ASCP) ADG Pathology  cke...@adgpath.com 
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Re: [Histonet] A Question About Paraffin Times

[cynthia haynes via Histonet]

Please don't leave the tissue in the hot wax.They will harden and be brittle. 
Cynthia James H.T 

Sent from Yahoo Mail on Android 
 
  On Tue, May 16, 2017 at 10:50 AM, P Sicurello via 
Histonet wrote:   Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] Send-Outs

[Cartun, Richard via Histonet]

Do you have a Pathology Residency Program?  If so, good luck with a policy that 
states that requests will be sent out in 48 hours.  Our policy is 3-5 business 
days once we receive the request.  Having said that, most requests are 
processed in 2-3 days.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax



-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:53 AM
To: Cristi Rigazio
Cc: Histonet
Subject: Re: [Histonet] Send-Outs

Really? You want to compare your lab to Amazon? If you have stock on-hand, 
located in one place, have robots to retrieve it, people who do nothing but 
pack boxes and trucks standing by to take any order at any time of day, then 
you are in the league of Amazon and can receive an order and send it out within 
an hour.

However most of us first need to find the material to send out - slides? where 
are they? Pathologist, resident? waiting to be filed? Removed from file by 
someone else? Can't find anything in THAT office...Same with blocks - not in 
file, is there a card telling who took it? Maybe for recuts, maybe for 
research, then back into the batch to be filed. Look 5 different places. Frozen 
tissue is usually easier but, whoops...already sent some out for another 
requested test...

Anyway, that is what my experience is!

We don't have any set time requirement. It could be an hour or a few days 
depending on if can easily find the material they want to send. Usually it is 
within a day.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 15, 2017 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Send-Outs

Hi Histoland!

We have been reviewing our send out procedures and I have been tasked with 
researching what TAT's other institutions define.  Currently, we allow 48 hours 
for the preparation of "orders" to the time sent out.  Is this reasonable?  Do 
other facilities allow more or less time?

I feel like Amazon can get something to my door step in a matter of hours, so I 
must be missing something obvious in our steps that could be simplified, but I 
can't put my finger on it.  Any feedback on what others are doing is greatly 
appreciated.

Sincerely,
Cristi
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[Histonet] Extra Large Cassette Clamps for Microtomes

[Sandra Cheasty via Histonet]

Hello all,
I am looking for opinions on giant block clamps for the Leica 
RM2235 and Microm HM355S microtomes.

* I know CellPath makes one, are their others?

* Is it easy to swap them out for a regular clamp, or is it advisable 
to dedicate one microtome for these huge cassettes.
Thank you!
Sandy


Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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Re: [Histonet] A Question About Paraffin Times

[Rene J Buesa via Histonet]

I consider taking out → freezing →  melting → casting blocks is worse than 
leaving the tissues in molten paraffin.It seems that your Monday tissue 
processing ends on Saturday. I suggest to process your tissues with a "delay" 
(most tissue processers have this feature) and leave  them more time in 
formalin and make coincide their ending time with your Monday start time.René 

On Tuesday, May 16, 2017 11:51 AM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] Send-Outs

[Morken, Timothy via Histonet]

Really? You want to compare your lab to Amazon? If you have stock on-hand, 
located in one place, have robots to retrieve it, people who do nothing but 
pack boxes and trucks standing by to take any order at any time of day, then 
you are in the league of Amazon and can receive an order and send it out within 
an hour.

However most of us first need to find the material to send out - slides? where 
are they? Pathologist, resident? waiting to be filed? Removed from file by 
someone else? Can't find anything in THAT office...Same with blocks - not in 
file, is there a card telling who took it? Maybe for recuts, maybe for 
research, then back into the batch to be filed. Look 5 different places. Frozen 
tissue is usually easier but, whoops...already sent some out for another 
requested test...

Anyway, that is what my experience is!

We don't have any set time requirement. It could be an hour or a few days 
depending on if can easily find the material they want to send. Usually it is 
within a day.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Send-Outs

Hi Histoland!

We have been reviewing our send out procedures and I have been tasked with 
researching what TAT's other institutions define.  Currently, we allow 48 hours 
for the preparation of "orders" to the time sent out.  Is this reasonable?  Do 
other facilities allow more or less time?

I feel like Amazon can get something to my door step in a matter of hours, so I 
must be missing something obvious in our steps that could be simplified, but I 
can't put my finger on it.  Any feedback on what others are doing is greatly 
appreciated.

Sincerely,
Cristi
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[Histonet] A Question About Paraffin Times

[P Sicurello via Histonet]

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] cassette printers - multi color cassettes and network ready-

[Blazek, Linda via Histonet]

Cheryl,
We use the Primera printer and have had great success with it.  It's small and 
reliable.  You can get it from http://cwsincorp.com/

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Cheryl via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 3:57 PM
To: Histonet
Subject: [Histonet] cassette printers - multi color cassettes and network ready-

What are you using for your cassette printer?  The Leica IP-C is a familiar 
model but it is SO BiG.
We need 3-5 colors of cassettes, and it has to network so we can have multiple 
users.  We print two lines (number and name) with hopes to go to full bar 
coding in the near future. Looking for any/all input - vendors welcome, too. 
Cheryl Kerry, HT(ASCP) ADG pathologycke...@adgpath.com 
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