We do a delay protocol as well. The tissue samples will sit in formalin and start processing Sunday night for a normal amount of time to come off Monday morning. If this is not a possibility, it would be better to let them harden in the paraffin than to leave them in the hot paraffin, as this will lead to the tissue becoming brittle and very difficult to section.
HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX ________________________________________ From: P Sicurello [[email protected]] Sent: Tuesday, May 16, 2017 11:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
