[Histonet] RE: IHC on free floating slices of brain
Hello Walter, Many years ago I did Timm's Silver stain on free-floating rat brain slices. The rats were perfused before removal and freezing of the brain on dry ice. I then would cut slices in a cryostat and float the sections in saline until ready to stain. I would imagine that it might be a bit costly to do IHC on free floating tissue sections because enough reagent and antibody solutions have to be used for each step. Not sure I can be of any help but email if you have any questions. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Walter Benton wben...@cua.md Sent: Tuesday, August 19, 2014 6:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on free floating slices of brain Does anyone have information they could share on performing IHC on whole mount, sledge microtome free floating slices of brain? A Neuropathologist colleague is interested in doing this. Thanks in advance for your help. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.comhttps://www.cua.md/owa/chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Is there a Law for refusal of pathology services.
Also, legally speaking, if it isn't documented, it didn't happen in the eyes of the law. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Sue suetp...@comcast.net Sent: Monday, August 18, 2014 9:43 PM To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] Is there a Law for refusal of pathology services. I agree, if you do not document that a specimen was removed most likely insurance will deny the clain. SPaturzo TJU ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bone saw for cutting slabs
Most hospitals that I've worked at that needed to cut bone used a Stryker bone saw. The pathologists never mentioned damaged cartilage. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of M.O. modz9...@gmail.com Sent: Tuesday, August 19, 2014 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone saw for cutting slabs Histoland! Happy Tuesday! I just wanted to get your feedback on cutting slabs from human femora for histopathological analysis. At them moment we are just using a hack saw to cut 7mm slabs from femora. We notice some marks on the cartilage from sawing, so when we cut the tissue down after decalcification for histological preparation, we cut the thickness down to 4mm and remove the damaged tissue. Would using some sort of bone saw damage the tissue even more or would it be comparable to using a hack saw? Is there a saw that you recommend that is precise and easy to handle that doesn't damage tissue greatly? Thank you so much for your help! Sincerely, Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dubin Johnson syndrome
What tissue are you staining? Liver? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of rashmil_histotechnol...@yahoo.com rashmil_histotechnol...@yahoo.com Sent: Wednesday, August 13, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dubin Johnson syndrome Hello, How specific is Fontana stain for Dubin Johnson syndrome? Thanks, Rashmil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Bubble problems!
Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Melissa Ng melissa...@spdscientific.com Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: multitissue control block
What is it that you want to know? I used to make frozen multiple tissue control blocks for ER/PR many years ago before these antibodies could be used for FFPE tissues. You could do the same thing with paraffin embedded tissue. I've made multi-tissue blocks using a skin biopsy punch tool to obtain small amounts of paraffin-embedded tissues to be used in the control block. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Ortiz, Debra debra.or...@advocatehealth.com Sent: Friday, July 25, 2014 3:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] multitissue control block Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il debra.or...@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cytokeratin AE1/AE3
What are you using for HIER? Citrate buffer? What pH? I use a low pH citrate buffer at 95C for 20 minutes and get good results. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Burnett, Brandy bburn...@capecodhealth.org Sent: Thursday, July 10, 2014 3:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically in-between the cells. We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Best fixative for whole brains
What species of brain are we talking about? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET) kemlo.rogers...@nhs.net Sent: Wednesday, July 02, 2014 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best fixative for whole brains Dear All, Which is the best fixative for whole brains? Obviously must be able to carry out tinctural stains and immunoperoxidase procedures. Kemlo Rogerson Chartered Scientist Liverpool UK This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology as art!
They're actually very pretty!! I'm going to check out this museum. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Emily Brown talulahg...@gmail.com Sent: Friday, June 27, 2014 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not?
While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on HE stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: Podawiltz, Thomas tpodawi...@lrgh.org Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on HE stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
Hmm. Maybe the company who manufactured our recycler went out of business or we got a lemon. As soon as we abandoned recycling and went back to new reagents, the stains and processing were perfect. Go figure. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce K. joyce.we...@emoryhealthcare.org Sent: Thursday, June 26, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) j...@cdc.gov Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems
[Histonet] RE: Back up Tissue processor
You will need to validate whatever programs you have on the back-up processor. Run cassettes of tissue representative of what you usually process on a day to day basis. Embed, cut and stain with HE and have your pathologist review the slides for satisfactory results. Document the programs, tissues and results. I create my own form for this. I would suggest if you're not going to use the processor on a daily basis that you run a dummy process (no cassettes) at least once a week to make sure the solutions are pumping into the retort. You don't want to be surprised with a broken back-up machine if your main processor is down! Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Abbott, Tanya tanyaabb...@catholichealth.net Sent: Wednesday, June 18, 2014 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Back up Tissue processor Does anyone keep a back up tissue processor in your lab? And if so, what sort of requirements are there for CAP for insuring it is working properly, etc? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special Stainers
You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa rjbu...@yahoo.com Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day René J. On Monday, June 9, 2014 11:03 AM, Adesupo, Adesuyi (Banjo) abades...@nrh-ok.com wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: re: Documenting special stain control blocks
I did the same thing. We've had no problem passing inspections. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of kathy.mach...@lpnt.net kathy.mach...@lpnt.net Sent: Monday, June 09, 2014 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Documenting special stain control blocks I made up a form for the special stain/IHC controls for this that lists antibody/special stain, type of tissue, Case #, and date put in use. The pathologist looks at one stained slide, which is kept with the controls, and signs off on form. I hope this works!!! Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3868 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: GI biopsies and special stains
I agree. However, if you only receive a few GI biopsies a day that might me feasible. 95% of my work is GI biopsies. I live in an area with a very large immigrant population which tends to have h.pylori infection (from what I've seen). On any given day over 50% of the gastric biopsies could be h.pylori positive. We see pre-ordering special stains as being efficient and saving the pathologist some time as well as increasing turnaround time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Bilger, Andrea abil...@wellspan.org Sent: Monday, June 09, 2014 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsies and special stains Tests need to be medically necessary, therefore, we do not auto order stains on all GI biopsies. We wait until the HE is screened and then order H Pylori by IHC on only those biopsies that need it. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 __ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica special/he stainer
I have the Leica Multistainer. It was purchased before I got here. The only thing I don't like is that the programs have to be compatible or else the machine alarms when you load a special stain onto the machine when it already has an HE going. There's a rather complicated way to get the programs to be compatible which was explained to me when I went for training. You have to use an excel spreadsheet and figure out what rinse is where during all the programs. Yes, it's something Leica's engineers have to work on. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Deloris Carter dels...@gmail.com Sent: Monday, June 09, 2014 5:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica special/he stainer Does anyone have any feedback on the Leica Multistainer? It stains HE as well as special stains. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Basement Lab
This is very true. When I started back in the 70s, the lab was in the basement right down the hall from the morgue. The only other department down there with us was the receiving department. The only difference from other histology labs was that this was the lab of the NAACLS accredited Histotechnology hospital based program in which I was a student. All the techs were graduates of the program and were involved in teaching the two students. I consider myself lucky that I trained this way rather than depending on the good graces of older histotechs teaching me at their whim. But, to be fair, I learned a great deal from the on-the-bench trained techs through the years. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa rjbu...@yahoo.com Sent: Monday, May 05, 2014 12:22 PM To: susan.wal...@hcahealthcare.com; suetp...@comcast.net; jrsmallw...@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement Lab Histology is a very OLD art and by the end of the XIX and up to the middle of the XX century the rule was to do histology near the morgue that was almost always in the hospital basement. Additionally also since the start the laboratory activity less respected was that of histotech, always considered inferior to the medical lab tech. Histology was practiced from the start by people trained to do histology and the histology techs were recruited from janitors, secretaries, or pathologists' family members. It was just a training in how to process, section and stain, like a flesh and bone instrument. This is a very long trend just now crumbling down because of CAP requirements and even perhaps as a sorts of human rights issue BUT there is always something remaining and nobody is surprised if the histology lab is relegated to the basement which is a non-premium real estate within the hospital. That is why! Oh those old days that in this case are not good old days! René J. On Monday, May 5, 2014 3:19 AM, susan.wal...@hcahealthcare.com susan.wal...@hcahealthcare.com wrote: Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone On May 3, 2014, at 12:10 PM, John Smallwood jrsmallw...@bell.net wrote: Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont. Can. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: I need opinions on Leica instruments
My lab is entirely Leica. Wouldn't have it any other way! Sent from my iPhone On Apr 24, 2014, at 10:19 AM, Michaela Lefaivre michaela.tourvi...@duke.edu wrote: We have the Bond III and love them! Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician IV Molecular Pathology Rm 4344 Purple Zone 919-684-4303 919-684-5095 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Deborah Shank [dsh...@chpnet.org] Sent: Thursday, April 24, 2014 10:00 AM To: Duddey, Aimee; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: I need opinions on Leica instruments BOND III is excellent, I have 6. Deborah Shank, Manager Mt.Sinai Beth Israel Ny,NY Immunopathology, Flow Cytometry, Immunohistochemistry, FISH Tel 212 420 4049 FAX 212 420 4087 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Thursday, April 24, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I need opinions on Leica instruments I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Melanin Bleaching...
Hi Sarah, I've bleached out melanin using a 5% Potassium Permangenate solution. It's very quick - about 15 to 30 minutes. Wash thoroughly in running tap water. I've done IHC after bleaching with no damage to the tissue. Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of sarah.dys...@stdavids.com sarah.dys...@stdavids.com Sent: Thursday, March 20, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleaching... So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: GI biopsies
Dr. Richmond, A large portion of our business is GI biopsies. We cut three levels per slide. We achieve this by cutting three ribbons at different levels and picking up two sections from each ribbon. If an H.pylori or AB/PAS is ordered we choose two sections from the middle ribbon. I check the quality of the slides before handing them out to the pathologists. I encourage the pathologists to share any unhappiness they have with our microtoming and work to improve the problem ASAP. It seems to me that skilled, caring histotechnologists plus good communication with the pathologists is the magic equation for excellent quality slides. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Bob Richmond rsrichm...@gmail.com Sent: Friday, March 14, 2014 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: GI biopsies An anonymous query: I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Well, I take what I can get. Many histotechs lack the skill, or are unwilling to lay more than one ribbon on a slide. I do like more than one level. A more serious problem is maintaining the quality of GI biopsy sections, one of the most difficult quality assurance issues in histopathology. (It was reviewed in J HIstotechnol last year - I can find the reference.) The problem is at its worst with duodenal biopsies, where some services never prepare an adequate slide. As the celiac disease fad spreads and bread is the Evil Food of the Year, I am really concerned about signing out duodenal biopsies where I can't even distinguish the lymphocytes. Edwards Deming lives! Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC Validation
On my IHC validation documents I have a space for comments. If I change anything in a protocol I make a note in the comments space. I haven't had any issue with any accrediting agencies. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Colbert lcolb...@pathmdlabs.com Sent: Tuesday, March 11, 2014 7:27 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Validation If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking tiny specimens
Eyelashes are pointed at the end. Other body hair is not. I would re-use my tiny paintbrush over and over. No need to pull out an eyelash every day! Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: John Kiernan jkier...@uwo.ca Sent: Friday, January 31, 2014 4:21 AM To: Barbara Tibbs; Cheryl Crowder; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] marking tiny specimens Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short eyebrow or forearm hair? JK = = = On 30/01/14, Barbara Tibbs barbara.ti...@accuratediagnosticlabs.com wrote: Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Cheryl Crowder ccrow...@vetmed.lsu.edu Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking tiny specimens
Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Cheryl Crowder ccrow...@vetmed.lsu.edu Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AW:cont. workflow
I'm curious. How many techs do you have working in your lab that you are able to complete 300 blocks including IHC, FISH, etc in 9 hours? Do your techs also gross? Do your techs put the slides together in folders with reports to be distributed to the pathologists? Thanks, Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Gudrun Lang gu.l...@gmx.at Sent: Tuesday, January 07, 2014 2:44 PM To: 'Lee Peggy Wenk' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW:cont. workflow Hi Peggy! You talk about a lab with many specimens, many blocks. What do you think is the number of blocks, where a change from batch to flow makes sense? We have about 300 blocks per day and work from 6.30 till 15.30. Usually we are ready with slide-distribution at noon. And we do also IHC, FISH, mutation analysis, IF , many fast frozens- so we are busy, when we are not dealing with routine-histology. And we are happy with our system. ;-) Fixation is all in a comparable range. TAT of biopsies is one day after entry (patients are called in usually one week afterwards from the clinicians...). And we have a very diversified workplace with comfortable workshifts. Advisers often hold up cont. workflow as best, but I think it must fit to the circumstances! Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Lee Peggy Wenk Gesendet: Montag, 06. Jänner 2014 18:31 An: Podawiltz, Thomas; Deanna Leslie; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Soaking artifact In most labs, the processor runs all night long. Someone comes in very early in the morning, empties the processor, starts the purge cycle, and then starts embedding a lot of blocks. The tissue processor then sits, doing nothing, from after the purge in the early morning, until sometime in the late afternoon, when all the tissues are loaded in for the overnight run. That means the tissue processor is doing nothing for up to 12 hours during the daytime. How about, besides the overnight run, we can set up 1 or 2 other shorter runs during the day, with the small biopsies. How about - process all the large tissues overnight, but keep the little biopsies that you grossed all afternoon in formalin until the morning. Empty out the large tissues, purge the process, embed the large tissues and start microtoming them. After the purge is done, put the small biopsies from the afternoon on the tissue processor, and process them for 1.5-2 hours (and if your processor is able to process half a load, do that to save on reagents). Then embed them and start microtoming them. Purge the processor again. In the mean time, all morning, collect the small biopsies again. After lunch, short process all the small biopsies from the morning. Embed them in the afternoon, purge the processor again, and load up the overnight load. If you don't have time to microtome the morning small biopsies (that you embedded in the afternoon), someone can microtome them in the morning the next day. Either with the large overnight load, or have someone else come in early, and while the other people are embedding the large tissue overnight load, they can be microtoming the small biopsies that were embedded in previous afternoon. Yes, all of this will mean changes: - staggered hours that people will be coming in - processing, embedding and microtoming continuously throughout the day - someone might have to microtome more than someone else, or might have to embed more than someone else. But if you rotate jobs around, over the months, everyone ends up doing the same amount of work overall. This is a type of continuous work flow, and does lead to faster turn around time and efficiency. When our lab changed to this system (we are an 1100 bed hospital, with lots of tissues from our ORs, from outside hospitals and clinics and doctors offices, so lots and lots of blocks), it took getting everyone involved - people accessioning, grossing, the histotechs, and the pathologists (they were not going to get their slides in numerical order). We have short cycles, the overnight long cycles, some rush cycles, and long cycles for breast and autopsy brain. We actually have more than 3 runs, but then we are working almost 24/7. During the time we were switching to continuous work flow, we had a few histotechs off on maternity and/or medical leaves. And we got a couple more clients, so the work load increased. But because of the continuous work flow, we were able to handle the additional work without having to hire anyone. Whereas before, we would process ALL the blocks overnight, and then would have lots of people embedding lots of blocks first
[Histonet] RE: Leica RM2255 block clamp
Spend the 292 pounds and buy a new clamp. A repaired clamp is never tight enough to hold the cassette as tightly as a new one. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Finlay Finlay finlay.fin...@glasgow.ac.uk Sent: Tuesday, November 19, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica RM2255 block clamp Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (£292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk mailto:finlay.fin...@glasgow.ac.uk The University of Glasgow Charity Number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] India Ink for inking surgical margin borders
FYI, laundry bluing works great to ink specimens and it's very cheap. Most grocery stores sell it. It may not be as dark as you like but if you need two colors for inking oriented specimens you could use it in addition to the India Ink. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Ed Crespo ecre...@cmblabs.com Sent: Thursday, November 07, 2013 3:14 PM To: wanda.sm...@hcahealthcare.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] India Ink for inking surgical margin borders Hi Wanda, Can you tell me the brand name or take a pic of the india ink you got at the art store? I bought one from dick blick art supply. Although it works (AND WAS CHEAP...LOL), we're always looking for a better, darker ink. Any brand suggestions would be great. Thank you in advance. ED Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecre...@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient(s), please notify the sender immediately by reply e-mail or by phone at (714) 657-7369 and delete this message and any attachments from your computer and any archival/backup copies. Thank you. On Oct 11, 2013, at 8:37 AM, wanda.sm...@hcahealthcare.com wanda.sm...@hcahealthcare.com wrote: We sued India Ink from an art supply store for years. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ed Crespo Sent: Thursday, October 10, 2013 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] India Ink for inking surgical margin borders I normally purchase India Ink from one of our vendors, but know it's also sold and used at artist supply shops. Does anyone know if I can used the artist india ink for Pathology use? Really, the only issue would be if the ink stays on the tissue during processing right? Please advise. Ed Crespo, CT(ASCP) Anatomic Pathology Manager Safety Officer 10700 Walker Street Cypress, CA 90630 phone: 714 880.3330 fax: 714 816.1511 email: ecre...@cmblabs.com cmblabs.com The contents of this e-mail message, including any attachments, are intended solely for the use of the person or entity to whom this e-mail is addressed. It contains information that may be privileged, proprietary, confidential, and protected from disclosure by applicable state and federal law. Any Protected Health Information (PHI) contained in this email is HIGHLY CONFIDENTIAL. It is intended for the exclusive use of the addressee. It is to be used only to aid in providing specific healthcare services to the patient(s). Any other use is a violation of Federal Law (HIPAA) and will be reported as such. If you are not the intended recipient of this message, or the employee or agent responsible for delivering it to the intended recipient, you are hereby advised that reading, disseminating, distributing, use, or copying
RE: [Histonet] Leica slide printers
I have had no problem with the Leica IPS. I'm fastidious about maintenance so perhaps that's the secret to my success. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton flna...@texaschildrens.org Sent: Tuesday, October 22, 2013 1:09 PM To: 'Catherine Simonson'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica slide printers If you are referring to the IPS slide writer, I would say continue looking it is very unreliable. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Monday, October 21, 2013 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica slide printers
The Leica IP S? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Catherine Simonson catherinesimon...@gmail.com Sent: Monday, October 21, 2013 4:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide printers I was wondering if anybody out there in Histoland had any experience with the new Leica slide printer? What are your thoughts? Thanks in advance, Catherine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cytology Procedure
Hi Haley, I've done cytoprep on and off for the past few decades. What exactly do you want to know about non-gyn prep? The first step would be to ask the pathologist what he/she would like prepared i.e. cell block, slides, stains. It's very important to keep the pathologist in the loop when setting all this up. If you could supply more information I could give you some ideas of what I have done in the past. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Huggins, Haley - MRMC haley.hugg...@dignityhealth.org Sent: Wednesday, October 16, 2013 6:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytology Procedure Hello all, I am looking for some assistance in Cytology procedures, specifically for non-gyn cytology. I am not as versed in Cytology as I am in Histology, so I am looking for some help with any procedures I might need. I know this is histonet, but I am sure a number of you have had to do cytology from time to time. Thanks in advance for any help. Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Body bags usage.
I don't work in a hospital now but when I did, on and off, for the past 30 years or so a body was always brought down to the morgue in a body bag or shroud. If a patient was morbidly obese the body was placed in a bariatric body bag and brought to the morgue. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton flna...@texaschildrens.org Sent: Friday, October 04, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body bags usage. When you have a patient that expires, do you require that the bodies be brought down in body bags for an autopsy or funeral home pick up? __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MOHS IPs
Hello Karen, Back in the old days of doing IHC manually on mostly fresh, frozen tissue I would immediately place the slide with the frozen section into a coplin jar of acetone to fix it. Keep the coplin jar of acetone in the cryostat. Leave it in the acetone for only 10 minutes. Try diluting the Protease 2 - 1:1 to make it gentler so it doesn't eat the tissue. This technique worked well when I was doing ER/PR on frozen breast tumors. Not sure it will work with skin but it's worth a try. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Bauer, Karen L. bauer.ka...@mayo.edu Sent: Tuesday, October 01, 2013 5:22 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] MOHS IPs Hi all, We are in the process of validating some antibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, but it could be darker. We will be increasing the Ab incubation time to see if that will help. As for the Pan Keratin, we cannot get it to work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in Histology, but this stain in MOHS is placed on fresh, unfixed tissue... by a manual drop method. Any time we've tried to use an enzyme retrieval, the tissue looks eaten up... even if we incubate if for a minute. Since the tissue is not fixed, I figured that no retrieval step would be needed, but the Pan Keratin refuses to work with or without retrieval. For those of you in MOHS labs that are using a manual staining method for Melan A and Pan Keratin, would you be willing to share your protocol with us? Thanks so much!! Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gastro Tissue for Validation of Processing Protocol
What I did when validating a processor for small biopsies was to cut tiny, biopsy-sized sections of tissue from larger specimens such as stomach, colon, skin, etc. We inquired at a local hospital to see if we could get specimens and they graciously donated tissues that otherwise would have been discarded. The validation process went well and I use the protocol to process patient biopsies at present. If you have access to these types of tissue, particularly stomach since that is what is your interest, you might try this technique. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Ian R Bernard ibern...@uab.edu Sent: Tuesday, September 17, 2013 11:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastro Tissue for Validation of Processing Protocol Our lab is looking to acquire actual (gastric) or alternate similar tissue to validate a gastro tissue processing protocol. Any suggestions on obtaining endoscopic biopsy specimens or alternate tissue types to validate the gastric biopsy protocol? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
