Re: [Histonet] P16

[Michael Kent via Histonet]

You might check Cancer Diagnostics, inc..  Their rep told us they have an IVD 
p16.

Best,
Mike
 
-Original Message-
From: Charles Riley via Histonet  
Sent: Wednesday, March 13, 2019 1:24 PM
To: Bernice Frederick 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] P16

We by predilute from BioGenix

On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Who is the best and current supplier for p16? We were using 
> biocare,but evidently it is no longer available from them. And no 
> Santa Cruz per my IHC tech.
> Thanks,
> Bernice
>
> Bernice Frederick
> Pathology Core Facility
> Robert H. Lurie Cancer Center
> 710 North Fairbanks Court
> Olson 8-421
> 312-503-3723
> b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu>
>
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Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] p16

[Joe Myers via Histonet]

To All Concerned Parties:
Please to be advised that the antibody to p16 offered by Biocare Medical, 
labeled ASR, is still available (in RTU-format only).

Joe Myers, M.S., CT(ASCP)QIHC
Senior Technical Sales Specialist
Biocare Medical

--

Message: 2
Date: Wed, 13 Mar 2019 16:11:09 +
From: Bernice Frederick 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] P16

Who is the best and current supplier for p16? We were using biocare,but 
evidently it is no longer available from them. And no Santa Cruz per my IHC 
tech.
Thanks,
Bernice

Bernice Frederick
Pathology Core Facility
Robert H. Lurie Cancer Center
710 North Fairbanks Court
Olson 8-421
312-503-3723
b-freder...@northwestern.edu
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Re: [Histonet] P16

[Charles Riley via Histonet]

We by predilute from BioGenix

On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Who is the best and current supplier for p16? We were using biocare,but
> evidently it is no longer available from them. And no Santa Cruz per my IHC
> tech.
> Thanks,
> Bernice
>
> Bernice Frederick
> Pathology Core Facility
> Robert H. Lurie Cancer Center
> 710 North Fairbanks Court
> Olson 8-421
> 312-503-3723
> b-freder...@northwestern.edu
>
> ___
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] P16

[Bernice Frederick via Histonet]

Who is the best and current supplier for p16? We were using biocare,but 
evidently it is no longer available from them. And no Santa Cruz per my IHC 
tech.
Thanks,
Bernice

Bernice Frederick
Pathology Core Facility
Robert H. Lurie Cancer Center
710 North Fairbanks Court
Olson 8-421
312-503-3723
b-freder...@northwestern.edu

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[Histonet] p16 and sperm morphology

[Nancy Schmitt via Histonet]

Hello-


1.   We are currently running P16 on the Leica Bond Max - are you running 
or reporting this any differently now that they are 510k FDA approved?


2.   Sperm Morphology- are your cytotechnologists reading and reporting out 
sperm morphology or are your clinical lab techs?


Responses appreciated:)

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001



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[Histonet] p16 dilution

[Echague, Caren Ann - MGH via Histonet]

Hi Histonetters,
Does any of you use p16 Cintec from Roche/Ventana?  We wanted to dilute this 
antibody but wasn't sure how much to dilute it to.  Figured we could save a lot 
by doing so.  We have been staining our p16 with no current dilution.  Any 
suggestions will help.  If there are any other vendors that offer p16 other 
than Roche, that would be useful to know.
Thanks!

Cae Aguilar
Histology Supervisor
California


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Re: [Histonet] p16 Antibody

[goodwin, diana via Histonet]

Hi, Laurie.  We use the Leica Bond and dispense it into an open vial for use on 
that instrument.  I suggest you contact the vendor for the IHC stainer you are 
using.  They should be able to tell you how to optimize the new Ventana Cintec 
P16 for use on your existing system.  

Good Luck!

Diana Goodwin
Anatomic Pathology Supervisor
Penn Medicine Princeton Medical Center
Ph:  609.853.6808
Fax: 609.853.6841

-Original Message-
From: Laurie Colbert [mailto:boz...@aol.com] 
Sent: Thursday, May 17, 2018 2:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

We have been using Ventana's p16 antibody and detection kit on an IHC stainer 
that is not Ventana's stainer.  We have been told that these reagents are now 
obsolete, but that they can be purchased in dispensers which are customized for 
Ventana's own stainer.  Can I simply just dispense their reagents into my own 
vials for my stainer?  I'm wondering if anyone else is dealing with this same 
issue.

Are there other companies that sell the p16 antibody?  The last time I checked 
(which was years ago), Ventana was the only one selling this.

Thanks,
Laurie Redmond
Path MD
Los Angeles, CA


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Re: [Histonet] p16 Antibody

[Greg Dobbin via Histonet]

Hi Laurie,
We are purchasing the antibody only from Roche/Ventana and optimizing it
for use with our detection system (Bond Refine Detection kit) and the
Bond-III stainer. Ask your Ventana person about buying the Ab only.
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


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[Histonet] p16 Antibody

[Laurie Colbert via Histonet]

We have been using Ventana's p16 antibody and detection kit on an IHC stainer 
that is not Ventana's stainer.  We have been told that these reagents are now 
obsolete, but that they can be purchased in dispensers which are customized for 
Ventana's own stainer.  Can I simply just dispense their reagents into my own 
vials for my stainer?  I'm wondering if anyone else is dealing with this same 
issue.

Are there other companies that sell the p16 antibody?  The last time I checked 
(which was years ago), Ventana was the only one selling this.

Thanks,
Laurie Redmond
Path MD
Los Angeles, CA
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[Histonet] P16 Antibody

[Jason McGough via Histonet]

We are looking to bring in P16 to do in house but are having a hard time 
getting the stain to be clean. We are having issues with positive staining on 
tissue that should be negative. We are working with a concentrated antibody 
that we have diluted out to 1:6000 (which is 6 times the amount suggested from 
vendors 1:200-1:1000). We might need to change clones or vendors and were 
wondering if you could tell us what kind of p16 (clone) you use for staining 
and possible what vendor you get it from? Any information or suggestions you 
have would greatly be appreciated. Thank you.


Jason McGough, HT(ASCP)

Operations Manager
Clinical Laboratory of the Black Hills
605-343-2267
jmcgo...@clinlab.com
www.clinlab.com

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[Histonet] P16

[Charles Riley via Histonet]

Does anyone order P16 from Biogenix? I am trying to purchase this antibody
from them but can not get ahold of the company to do so. If you do can you
please send me the link or phone number to contact them. Thanks

-- 

Charles Riley HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] p16 from Ventana

[Allie Becker via Histonet]

Does anybody have more information on what these changes are for the Roche p16?

Sent from my iPhone

> On Apr 5, 2017, at 16:07, Lynch, Kenia via Histonet 
>  wrote:
> 
> Hi all,
> 
> Roche will still have the p16 antibody, but there are a few changes taking
> place. Please reach out to your account manager for clarification.
> 
> Thanks,
> KCL
> 
> On Wed, Apr 5, 2017 at 3:47 PM, Teri Johnson via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Wait...what? When is Ventana p16 being discontinued?
>> 
>> Teri Johnson, HT(ASCP)QIHC
>> Manager, Clinical Trial Testing
>> 
>> T +1 760 516 5954
>> tejohn...@genoptix.com
>> 
>> Navigate BioPharma, Inc.
>> A Novartis Company
>> 1890 Rutherford Rd.
>> Carlsbad, CA  92008
>> USA
>> 
>> Date: Wed, 5 Apr 2017 01:54:06 +
>> From: Samantha Claypool 
>> To: "histonet@lists.utsouthwestern.edu"
>> 
>> Subject: [Histonet] Sigma p16
>> 
>> Hello Histonet!  Anyone out there using (or getting ready to use) the
>> p16(ink4a) antibody available from Sigma?  We currently use Ventana's but
>> will probably buy from Sigma once the Ventana product is discontinued.
>> Would love to hear from anyone with experience with Sigma.
>> 
>> Thanks,
>> Samantha
>> Huyer Laboratory Services
>> 
>> 
>> 
>> 
>> 
>> 
>> CONFIDENTIALITY NOTICE: The sender of this email is not an employee or
>> agent of, or a contractor or consultant to, Genoptix. This email has been
>> sent through the Genoptix email system as a transitional service provided
>> by Genoptix, Inc. (“Genoptix”) to Navigate BioPharma Services, Inc. The
>> sender of this email is not authorized to represent or to speak on behalf
>> of any member of Genoptix, nor to enter into any undertaking, arrangement
>> or agreement on behalf of any member of Genoptix. Any such undertaking,
>> arrangement or agreement shall not be binding on Genoptix. Genoptix accepts
>> no liability for the content of this email, or for the consequences of any
>> acts or omissions taken or not taken on the basis of the information
>> contained in this email or in any email sent from this email address. All
>> reasonable precautions have been taken to ensure no viruses or other
>> malware are present in this e-mail and Genoptix cannot accept
>> responsibility for any loss or damage arising from the use of this e-mail
>> or attachments and you are responsible for ensuring that your own virus
>> screening procedures are up to date and fit for purpose.
>> ___
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> 
> 
> 
> -- 
> *Kenia C. Lynch, MT, HT, QIHC*
> 
> Account Manager, North Carolina
> Roche Diagnostics Corporation
> 9115 Hague Road, PO Box 50457
> Indianapolis, IN 46250-0457
> 
> Tel.  +1-800-227-2155
> Cell  +1-208-972-4088
> kenia.lynch@roche.com
> 
> 
> [image: The Future of H]
> 
> 
> 
> *Confidentiality Note:* This message is intended only for the use of the
> named recipient(s) and may contain confidential and/or proprietary
> information. If you are not the intended recipient, please contact the
> sender and delete this message. Any unauthorized use of the information
> contained in this message is prohibited.
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Re: [Histonet] p16 from Ventana

[Lynch, Kenia via Histonet]

Hi all,

Roche will still have the p16 antibody, but there are a few changes taking
place. Please reach out to your account manager for clarification.

Thanks,
KCL

On Wed, Apr 5, 2017 at 3:47 PM, Teri Johnson via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Wait...what? When is Ventana p16 being discontinued?
>
> Teri Johnson, HT(ASCP)QIHC
> Manager, Clinical Trial Testing
>
> T +1 760 516 5954
> tejohn...@genoptix.com
>
> Navigate BioPharma, Inc.
> A Novartis Company
> 1890 Rutherford Rd.
> Carlsbad, CA  92008
> USA
>
> Date: Wed, 5 Apr 2017 01:54:06 +
> From: Samantha Claypool 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Sigma p16
>
> Hello Histonet!  Anyone out there using (or getting ready to use) the
> p16(ink4a) antibody available from Sigma?  We currently use Ventana's but
> will probably buy from Sigma once the Ventana product is discontinued.
> Would love to hear from anyone with experience with Sigma.
>
> Thanks,
> Samantha
> Huyer Laboratory Services
>
>
>
>
> 
>
> CONFIDENTIALITY NOTICE: The sender of this email is not an employee or
> agent of, or a contractor or consultant to, Genoptix. This email has been
> sent through the Genoptix email system as a transitional service provided
> by Genoptix, Inc. (“Genoptix”) to Navigate BioPharma Services, Inc. The
> sender of this email is not authorized to represent or to speak on behalf
> of any member of Genoptix, nor to enter into any undertaking, arrangement
> or agreement on behalf of any member of Genoptix. Any such undertaking,
> arrangement or agreement shall not be binding on Genoptix. Genoptix accepts
> no liability for the content of this email, or for the consequences of any
> acts or omissions taken or not taken on the basis of the information
> contained in this email or in any email sent from this email address. All
> reasonable precautions have been taken to ensure no viruses or other
> malware are present in this e-mail and Genoptix cannot accept
> responsibility for any loss or damage arising from the use of this e-mail
> or attachments and you are responsible for ensuring that your own virus
> screening procedures are up to date and fit for purpose.
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*Kenia C. Lynch, MT, HT, QIHC*

Account Manager, North Carolina
Roche Diagnostics Corporation
9115 Hague Road, PO Box 50457
Indianapolis, IN 46250-0457

Tel.  +1-800-227-2155
Cell  +1-208-972-4088
kenia.lynch@roche.com


[image: The Future of H]



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Re: [Histonet] p16 from Ventana

[Teri Johnson via Histonet]

Wait...what? When is Ventana p16 being discontinued?

Teri Johnson, HT(ASCP)QIHC
Manager, Clinical Trial Testing

T +1 760 516 5954
tejohn...@genoptix.com

Navigate BioPharma, Inc.
A Novartis Company
1890 Rutherford Rd.
Carlsbad, CA  92008
USA

Date: Wed, 5 Apr 2017 01:54:06 +
From: Samantha Claypool 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Sigma p16

Hello Histonet!  Anyone out there using (or getting ready to use) the 
p16(ink4a) antibody available from Sigma?  We currently use Ventana's but will 
probably buy from Sigma once the Ventana product is discontinued.  Would love 
to hear from anyone with experience with Sigma.

Thanks,
Samantha
Huyer Laboratory Services






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through the Genoptix email system as a transitional service provided by 
Genoptix, Inc. (“Genoptix”) to Navigate BioPharma Services, Inc. The sender of 
this email is not authorized to represent or to speak on behalf of any member 
of Genoptix, nor to enter into any undertaking, arrangement or agreement on 
behalf of any member of Genoptix. Any such undertaking, arrangement or 
agreement shall not be binding on Genoptix. Genoptix accepts no liability for 
the content of this email, or for the consequences of any acts or omissions 
taken or not taken on the basis of the information contained in this email or 
in any email sent from this email address. All reasonable precautions have been 
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[Histonet] p16

[Joanne Clark via Histonet]

Good Morning Histonetters, I am wondering how all of you using p16 from Roche 
on a Leica staining system are going to adapt to the changes coming from Roche. 
 Are you going to pay the exorbitant price for the new kit and list the test as 
an LDT in your reports, or are you going to source one of the ASR antibodies 
out there and use that route?  I just want to be sure I can re work a research 
antibody and report it as an ASR test in our reports without CAP giving us a 
hard time about it.
Thanks for the feedback.

Joanne Clark, HT
Director of Histology
Pathology Consultants of New Mexico




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[Histonet] p16 replacements?

[Cassie P. Davis via Histonet]

Hi Histonet Folks,

our current vendor  will be discontinuing carrying the antibody for 
p16. Does anybody have suggestions for replacing in it? We currently use it to 
stain for CIN. Many thanks in advance for sharing.


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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Re: [Histonet] P16

[Cartun, Richard via Histonet]

We purchase the antibody from Ventana and run it on our Bond Max platforms.  
During re-optimization, we found we could dilute it 1:10 (15 minutes) following 
"H2" (high pH) retrieval and still get excellent results.

Richard

Richard W. Cartun, MS, PhD
Director, Histology and the Martin M. Berman, MD Immunopathology and 
Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Department of Pathology and Laboratory Medicine
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax


From: Dessoye, Michael via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 08, 2016 1:14 PM
To: Paula; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] P16

Ventana/Roche bought out MTM which had the patent, so they're the only vendor 
you can get it from.  Since you're using Bond you obviously don't want it in a 
dispenser, but you can buy their manual staining kit and just use the antibody 
from that.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth 
Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River 
Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484


-Original Message-
From: Paula [mailto:plu...@biopath.org]
Sent: Wednesday, June 08, 2016 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and 
we use ready to use antibodies. Leica doesn't carry this antibody and so I was 
hoping someone can tell me which vendor carries it and that I can order it from.

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California




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Re: [Histonet] P16

[J B via Histonet]

Ventana medical systems is the only one I know of right now.

On Wed, Jun 8, 2016, 10:47 AM Baldwin, Kathy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Just saw someone reaching out for P16 was wondering if anyone would share
> their protocol for this antibody we are using Ventana/Roche and are stain
> is coming out weak :(
>
>
>
> Thanks
> S. Kathy Baldwin
> Histology/Cytology Supervisor
> Memorial Hospital and Health Care Center
> 800 West 9th St.
> Jasper, Indiana  47546
> Office 812-996-0210
> Fax 812-996-0232
>
>
>
>
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is
> for the sole use of the intended recipient(s) and may contain confidential
> and privileged information or otherwise protected by law. Any unauthorized
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> intended recipient, please contact the sender by reply e-mail and destroy
> all copies of the original message.
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[Histonet] P16

[Baldwin, Kathy via Histonet]

Just saw someone reaching out for P16 was wondering if anyone would share their 
protocol for this antibody we are using Ventana/Roche and are stain is coming 
out weak :(



Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232





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Re: [Histonet] P16

[Dessoye, Michael via Histonet]

Ventana/Roche bought out MTM which had the patent, so they're the only vendor 
you can get it from.  Since you're using Bond you obviously don't want it in a 
dispenser, but you can buy their manual staining kit and just use the antibody 
from that.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth 
Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River 
Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484


-Original Message-
From: Paula [mailto:plu...@biopath.org] 
Sent: Wednesday, June 08, 2016 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and 
we use ready to use antibodies. Leica doesn't carry this antibody and so I was 
hoping someone can tell me which vendor carries it and that I can order it 
from.  

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California




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Re: [Histonet] P16

[Jeanne Blanchard via Histonet]

We use the p16 from Ventana/Roche, but of course it comes in their dispenser.

Jeanne Blanchard, CT (ASCP)CM




-Original Message-
From: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, June 08, 2016 11:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and 
we use ready to use antibodies. Leica doesn't carry this antibody and so I was 
hoping someone can tell me which vendor carries it and that I can order it 
from.  

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California

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[Histonet] P16

[Paula via Histonet]

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III
and we use ready to use antibodies. Leica doesn't carry this antibody and so
I was hoping someone can tell me which vendor carries it and that I can
order it from.  

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California

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[Histonet] p16 Nordi QC

[Eridana via Histonet]

There is a fantastic resource for antibody assessment and optimization and they 
have done p16 as well as many many other antibodies. Their results of testing 
is free on line.   p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009 
with many different companies antibodies.

Here is the list of their epitopes http://www.nordiqc.org/epitopes.htm

Donna Harclerode, HT, ASCP, HTL, QIHC
Lead Histotechnologist
VA San Diego, CA

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[Histonet] P16

[Charles Riley via Histonet]

Has anyone found a way to do p16 staining without purchasing anything from
Ventana?

My company wants to do P16 but refuses to by any ventana products and I
have explained it is a waste of money to keep testing the antibodies from
all the other companies.

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] P16 alternative

[Charles Riley via Histonet]

Does anyone know an alternative to Ventana's P16 antibody for testing for
viral inclusions like HPV using IHC?

-- 

Charles Riley HT(ASCP)CM
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[Histonet] P16

[kalkhenaizi . via Histonet]

Hello Histonetters,

Happy Friday!

I need p16 antibody but not sure which vendor provide reliable antibody.
I'd appreciate any recommendation.

I also need melanoma and prostate ca control blocks, I can exchange with
the once I have.

Thank you and have a nice weekend.

Kadhem


Kadhem Alkhenaizi, MS
HTL (ASCP) MB, QIHC
Lab Manager
ExpressMed Labs
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RE: [Histonet] p16

[Piche, Jessica]

Thanks!

From: Shannon Kleiner [mailto:shk...@bellin.org]
Sent: Wednesday, March 04, 2015 4:12 PM
To: histonet@lists.utsouthwestern.edu; Piche, Jessica
Subject: Re: [Histonet] p16

Hello Jessica,

We use Cintec p16 on our Bond Max. Here is a link you may find useful

http://www.ventana.com/cervical

Shannon Logan HTL (ASCP)
Green Bay, WI



 Piche, Jessica jpi...@wtbyhosp.orgmailto:jpi...@wtbyhosp.org 3/4/2015 
 1:38 PM 
Hi Everyone,

I was wondering if people are using the p16 antibody and if so where are you 
getting it from?

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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[Histonet] p16

[Piche, Jessica]

Hi Everyone,

I was wondering if people are using the p16 antibody and if so where are you 
getting it from?

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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Re: [Histonet] p16

[Michael Ann Jones]

Ventana - we buy the large test dispenser.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 3/4/15, 12:38 PM, Piche, Jessica jpi...@wtbyhosp.org wrote:

Hi Everyone,

I was wondering if people are using the p16 antibody and if so where are
you getting it from?

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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Re: [Histonet] p16

[Shannon Kleiner]

Hello Jessica, 
 
We use Cintec p16 on our Bond Max. Here is a link you may find useful

http://www.ventana.com/cervical
 
Shannon Logan HTL (ASCP)
Green Bay, WI 

 
 
 Piche, Jessica jpi...@wtbyhosp.org 3/4/2015 1:38 PM 
Hi Everyone,

I was wondering if people are using the p16 antibody and if so where are you 
getting it from?

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



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information that is legally privileged. This information is intended only for 
the use of the individual or entity named above. The authorized recipient of 
this information is prohibited from disclosing this information to any other 
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intended recipient, you are hereby notified that any disclosure, copying, 
distribution or action taken in reliance on the contents of these documents is 
strictly prohibited. If you have received this information in error, please 
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RE: [Histonet] p16 Antibody

[Sarah.Dysart]

Ventana unfortunately has the market on the good clone...you can dilute it 
out pretty far (it comes pre-diluted) and still get good results however...


Sarah Dysart  BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
North Austin Medical Center
12221 North Mopac Expressway
Austin, TX  78758
512-901-1220 (lab)
512-901-6659 (office)



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, January 14, 2015 8:41 AM
To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. 

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but 
our pathologist told us that the antibody we are using right now is not good. 
I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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RE: [Histonet] p16 Antibody

[Sebree Linda A]

We use one from BD, Veronique, with good results. 

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but 
our pathologist told us that the antibody we are using right now is not good. 
I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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RE: [Histonet] p16 Antibody

[Burnett, Brandy]

We use the Ventana (INK4a) clone as well. What dilution do you use?

Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburn...@capecodhealth.org


Expert physicians. Quality hospitals. Superior care. 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, January 14, 2015 10:01 AM
To: lseb...@uwhealth.org; veronique.bar...@gmail.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

Ventana unfortunately has the market on the good clone...you can dilute it 
out pretty far (it comes pre-diluted) and still get good results however...


Sarah Dysart  BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin 
Medical Center
12221 North Mopac Expressway
Austin, TX  78758
512-901-1220 (lab)
512-901-6659 (office)



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, January 14, 2015 8:41 AM
To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. 

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but 
our pathologist told us that the antibody we are using right now is not good. 
I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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~~
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you have received this email in error  contact the Help Desk for Cape Cod 
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RE: [Histonet] p16 Antibody

[Martha Ward-Pathology]

We dilute ours 1:5 from the predilute and run it on the Bond 3.   It works very 
well.

 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Wednesday, January 14, 2015 10:15 AM
To: 'sarah.dys...@stdavids.com'; lseb...@uwhealth.org; 
veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use the Ventana (INK4a) clone as well. What dilution do you use?

Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburn...@capecodhealth.org


Expert physicians. Quality hospitals. Superior care. 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, January 14, 2015 10:01 AM
To: lseb...@uwhealth.org; veronique.bar...@gmail.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

Ventana unfortunately has the market on the good clone...you can dilute it 
out pretty far (it comes pre-diluted) and still get good results however...


Sarah Dysart  BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin 
Medical Center
12221 North Mopac Expressway
Austin, TX  78758
512-901-1220 (lab)
512-901-6659 (office)



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, January 14, 2015 8:41 AM
To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. 

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but 
our pathologist told us that the antibody we are using right now is not good. 
I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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[Histonet] p16 Antibody

[Véronique Barrès]

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining,
but our pathologist told us that the antibody we are using right now is not
good. I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
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[Histonet] p16 [INK4] clone G175-405

[Tiana Baskin]

Hi Histonetters,

I am trying to work up the Biogenex p16 on FFPE tissue and I am not having much 
luck. Does anyone have any experience with this antibody and getting it to 
work. I have tried a wide range of dilutions and epitope retrieval (both heat 
and enzyme). Any suggestions?

Thanks in advance :)

Tiana
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[Histonet] p16 from XT to Ultra

[Davis, Cassie]

Hi histoland,

 You guys were wonderful helping me with the MUM-1 on the XT a few 
months ago, I am hoping you can help me now. I am trying to optimize the p16 on 
the Ultra and having a little trouble. It is B-E-A-utiful on the XT but does 
not want to play nice on the Ultra. Any tips...Anyone?

Cassandra Davis
cda...@che-east.org
302-575-8095



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[Histonet] p16 from XT to Ultra

[Algeo, Lacie A]

 Laboratories Division of 
Transplantation and Hepatic Pathology nalesni...@upmc.edu





--

Message: 3
Date: Thu, 28 Aug 2014 12:37:46 -0400
From: Davis, Cassie cda...@che-east.org
Subject: [Histonet] p16 from XT to Ultra
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
be7af02009c3a74f9b8344e70f3655cb0ab5ce3...@chexcms01.one.ads.che.org
Content-Type: text/plain; charset=iso-8859-1

Hi histoland,

 You guys were wonderful helping me with the MUM-1 on the XT a few 
months ago, I am hoping you can help me now. I am trying to optimize the p16 on 
the Ultra and having a little trouble. It is B-E-A-utiful on the XT but does 
not want to play nice on the Ultra. Any tips...Anyone?

Cassandra Davis
cda...@che-east.org
302-575-8095



Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health East 
and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential.  Any 
unauthorized review, use, disclosure, or distribution is prohibited. If you are 
not the intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.



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End of Histonet Digest, Vol 129, Issue 36
*



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[Histonet] p16

[Nancy Schmitt]

We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container 
when received.

Comes from Dako.



Nancy Schmitt HT, MLT(ASCP)

Histology Coordinator

Dubuque, IA

*



Message: 12

Date: Thu, 7 Aug 2014 13:06:37 +

From: Burnett, Brandy 
bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org

Subject: RE: [Histonet] RE: p16

To: Weems, Joyce K. 
joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org, 
'Ronda

  Mire'  rm...@cvpath.orgmailto:rm...@cvpath.org

Cc: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

  
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu,  
sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

  sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

Message-ID:

  
c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.orgmailto:c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.capecodhealth.org



Content-Type: text/plain; charset=us-ascii



Do you know what the dilution is from Biocare, and how many tests per vial?





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RE: [Histonet] p16

[Campbell, Tasha M.]

Are you sure Dako has p16?  

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Thursday, August 07, 2014 1:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16

We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the
container when received.

Comes from Dako.



Nancy Schmitt HT, MLT(ASCP)

Histology Coordinator

Dubuque, IA

*



Message: 12

Date: Thu, 7 Aug 2014 13:06:37 +

From: Burnett, Brandy
bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org

Subject: RE: [Histonet] RE: p16

To: Weems, Joyce K.
joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org
, 'Ronda

  Mire'  rm...@cvpath.orgmailto:rm...@cvpath.org

Cc:
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.
edu

 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.
edu,
sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

  sarah.dys...@stdavids.commailto:sarah.dys...@stdavids.com

Message-ID:

 
C7CEB8BE7DF69446817C237018344D0004A719@FHEXMB1.cchdomain1.capecodhealth
.orgmailto:c7ceb8be7df69446817c237018344d0004a...@fhexmb1.cchdomain1.ca
pecodhealth.org



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Do you know what the dilution is from Biocare, and how many tests per
vial?





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[Histonet] P16

[Nancy Schmitt]

Actually comes from Roche..sorry about that.

Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator



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[Histonet] p16

[Sarah.Dysart]

Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I would 
prefer it to be undiluted (concentrate) so that we can titrate out the antibody 
ourselves.  We need it to have publications that would back this up and/or 
stained slide pictures I can show our pathologist.
Thanks

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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Re: [Histonet] p16

[Tony Auge]

I have had excellent results with P16 (JC8) from Santa Cruz Biotech.

http://www.scbt.com/datasheet-56330-p16-jc8-antibody.html



Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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[Histonet] p16

[Nancy Schmitt]

Good Morning!

Where are you currently obtaining p16 from?

Thanks a bunch!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories
Please visit our website: http://www.uclaccess.com/




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[Histonet] P16

[tahseen]

Hi All,
I am looking P16 for HP V in Sq.ca of oral cavity, which company and 
clone will be good. I use Ab cam pathologist not comfortable.

Thanks,
Muhammad Tahseen
Supervisor Histology
SKMCHRC Lahore Pakistan

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[Histonet] p16

[Gary Gracie]

Dear Muhammad,

We are using MTM p16. This is now supplied by Roche cat#
06594441001.

It is only sold as a pre-dilute but I dilute 1/2 and it still works very well.

Regards

Gary Gracie
Senior Technical Officer
IHC Laboratory
Anatomical Pathology
St Vincents Hospital
Sydney Australia


Hi All,
I am looking P16 for HP V in Sq.ca of oral cavity, which company and
clone will be good. I use Ab cam pathologist not comfortable.
Thanks,
Muhammad Tahseen
Supervisor Histology
SKMCHRC Lahore Pakistan

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[Histonet] p16 control tissue

[Laurie Colbert]

We are going to be running the p16 antibody from Biocare (RUO) on skin 
specimens.  What type of tissue should I run for a positive control?  The data 
sheet says normal testis..

Laurie Colbert
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[Histonet] p16 on the Bond

[Martha Ward-Pathology]

I am having trouble posting to the histonet for some reason.  I am looking for 
condition and incubation times for p16 on the Bond.  Our new pathologist says 
our p16 looks overstained to him and not at all what he is accustomed to seeing 
and I am trying to get the staining more to his liking, without much success.

Thanks in advance for the help.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 





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Re: [Histonet] p16 on the Bond

[Richard Cartun]

We dilute the CINtec p16 RTU antibody (Ventana/Roche) 1:10 (15' primary 
antibody incubation) and use high pH retrieval (H2) for 10' on the Bond Max.
Richard



Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
 Martha Ward-Pathology mw...@wakehealth.edu 6/21/2013 12:07 PM 


I am having trouble posting to the histonet for some reason.  I am looking for 
condition and incubation times for p16 on the Bond.  Our new pathologist says 
our p16 looks overstained to him and not at all what he is accustomed to seeing 
and I am trying to get the staining more to his liking, without much success.

Thanks in advance for the help.



Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  







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Re: [Histonet] P16 on Leica Bond

[Patsy Ruegg]

Really 1:4?___
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[Histonet] P16 on Leica Bond

[Kathryn Maddox]

If anyone out there is performing  p16 on the Leica Bond immunostainer, could 
you please tell me where you purchase your antibody and what protocol you are 
using?
Thanks so much!
Kathy Maddox HT{ASCP}
Lake Charles, Louisiana
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[Histonet] p16

[Cynthia Pyse]

Happy Wednesday Everyone

Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana? 

 

One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors. Every other IHC works consistently but the p16, anyone have any
thoughts? I do make the DAB fresh daily as suggested in the instructions, it
is the same kit that has been running. The only thing I can think of is we
do sometimes run the p16 twice a day without making fresh DAB, but the
instructions say it can be used within a 24 hour period. 

 

This problem is driving me crazy! It just doesn't make any sense. Any help
would be appreciated. Thanks in advance.

Cindy

 

Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cp...@x-celllab.com

 

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FW: [Histonet] p16

[Cynthia Pyse]

Yes we monitor the temp and humidity. Retrieval is made fresh for every run.
We run the PT for the p16 in a water bath since we only run a few slides at
a time, but the water bath temp in also monitored. I should say that there
is some staining in these slides just not what the control usually looks
like. If we repeat the slides they do stain correctly. There is no
difference in the slide prep or the staining procedure. Any ideas.
Cindy 

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com

-Original Message-
From: Ross Benik [mailto:r...@premierlab.com] 
Sent: Wednesday, February 06, 2013 1:26 PM
To: Cynthia Pyse
Subject: RE: [Histonet] p16

Do you monitor the temperature and humidity inside the Link48? How often do
you prepare your retrieval solution/are you using the PT mods?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Wednesday, February 06, 2013 11:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16

Happy Wednesday Everyone

Has anyone been having consistency problems with their p16 staining since
MTM has been taken over by Ventana?



One day the procedure works another day it doesn't. Same procedure, no
variation in the temperature on pretreatment, run on the Dako 48, with no
errors. Every other IHC works consistently but the p16, anyone have any
thoughts? I do make the DAB fresh daily as suggested in the instructions, it
is the same kit that has been running. The only thing I can think of is we
do sometimes run the p16 twice a day without making fresh DAB, but the
instructions say it can be used within a 24 hour period.



This problem is driving me crazy! It just doesn't make any sense. Any help
would be appreciated. Thanks in advance.

Cindy



Cindy Pyse, CLT, HT (ASCP)

Laboratory Manager

X-Cell Laboratories

20 Northpointe Parkway Suite 100

Amherst, NY 14228

716-250-9235 etx. 232

e-mail cp...@x-celllab.com



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Re: [Histonet] P16 on Leica Bond

[Richard Cartun]

When we obtained the p16 antibody from mtm Laboratories we used it a dilution 
of 1:10 on the Bond Max. I hope that the concentration will not change now that 
Ventana is selling it.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Assistant Director, Anatomic Pathology
Director, Biospecimen Collection Programs
Hartford Hospital
80 Seymour Street
Hartford, CT 06012
(860) 545-1596
(860) 545-2204 Fax

 Kathryn Maddox 02/06/13 12:13 PM  
If anyone out there is performing p16 on the Leica Bond immunostainer, could 
you please tell me where you purchase your antibody and what protocol you are 
using? 
Thanks so much! 
Kathy Maddox HT{ASCP} 
Lake Charles, Louisiana 
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[Histonet] P16 and HPV

[Barbara.Crill]

I've been told that the HPV testing will be discontinued as of end of year. HPV 
expresses the P16 gene but so does other type of cancers associated with HPV.


It is possible to have a P16 pos and HPV negative test.
But is it not possible to have HPV pos and P16 neg.  if HPV is positive then 
the P16 is also positive.


So the P16 cannot be used to replace the HPV testing can it?


Antoinette Crill
TEAM LEADER ANATOMIC PATHOLOGY


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[Histonet] p16

[Gerald Davydov]

P16 stain demonstrates week nuclear stain. Any ideas how to correct?

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Re: [Histonet] p16

[Richard Cartun]

In order to troubleshoot, one would need to know the following:

Test tissue
Fixation
Antibody clone, dilution, and incubation time
Detection system
Antigen retrieval
Positive control tissue

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Gerald Davydov geralddavy...@gmail.com 7/20/2012 9:17 AM 
P16 stain demonstrates week nuclear stain. Any ideas how to correct?

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RE: [Histonet] p16

[Britton, Josette C]

Where is everyone getting their p16 IVD antibody from?

Josie Britton HT
Cheshire Medical Center
580 Court Street
Keene, NH 03431

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, July 20, 2012 12:17 PM
To: Gerald Davydov; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] p16

In order to troubleshoot, one would need to know the following:

Test tissue
Fixation
Antibody clone, dilution, and incubation time
Detection system
Antigen retrieval
Positive control tissue

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Gerald Davydov geralddavy...@gmail.com 7/20/2012 9:17 AM 
P16 stain demonstrates week nuclear stain. Any ideas how to correct?

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RE: [Histonet] p16

[McMahon, Loralee A]

Roche/Ventana.  they bought MTM labs and then raised the price. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Britton, Josette C 
[jcbrit...@cheshire-med.com]
Sent: Friday, July 20, 2012 12:57 PM
To: Richard Cartun; Gerald Davydov; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16

Where is everyone getting their p16 IVD antibody from?

Josie Britton HT
Cheshire Medical Center
580 Court Street
Keene, NH 03431

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, July 20, 2012 12:17 PM
To: Gerald Davydov; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] p16

In order to troubleshoot, one would need to know the following:

Test tissue
Fixation
Antibody clone, dilution, and incubation time
Detection system
Antigen retrieval
Positive control tissue

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Gerald Davydov geralddavy...@gmail.com 7/20/2012 9:17 AM 
P16 stain demonstrates week nuclear stain. Any ideas how to correct?

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RE: [Histonet] p16

[Troutman, Kenneth A]

The only IVD p16 available in the US is from MTM.  However, Roche just bought 
MTM so you will have to order it through Ventana.

Their Customer support number is 800-227-2155.

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232
*(Office)  615-875-3311
*(Lab) 615-343-9134
ashley.trout...@vanderbilt.edumailto:ashley.trout...@vanderbilt.edu

Message: 20
Date: Fri, 20 Jul 2012 12:57:31 -0400
From: Britton, Josette C 
jcbrit...@cheshire-med.commailto:jcbrit...@cheshire-med.com
Subject:
To: Richard Cartun rcar...@harthosp.orgmailto:rcar...@harthosp.org, 
Gerald Davydov
geralddavy...@gmail.commailto:geralddavy...@gmail.com,  
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Message-ID:

f644cd64b2313f43bb3d2496b454cdac0a2db...@cmc-ex01.cheshire-med.commailto:f644cd64b2313f43bb3d2496b454cdac0a2db...@cmc-ex01.cheshire-med.com
Content-Type: text/plain;charset=us-ascii

Where is everyone getting their p16 IVD antibody from?

Josie Britton HT
Cheshire Medical Center
580 Court Street
Keene, NH 03431

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[Histonet] P16

[Nancy Schmitt]

What are folks using for P16?

Thanks for your help-
Nancy



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[Histonet] p16 Protocol....

[Tom McNemar]

Hello all,

I was wondering if anyone would be willing to share a protocol for p16 
(Biogenix pre-dilute) antibody on the Benchmark XT.  I'm having a little 
trouble getting anything to stain.  Thanks.


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org



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[Histonet] P16 and ProExC Substitutions

[Rick and Sue Boen]

Hi All,

 

Now that MTM has made P16 very expensive and TriPath is have problems
producing ProExC, what's everybody using on ffpe tissue? We were told by
TriPath/BD that ProExC will probably not be available until late June.

 

Rick Boen, BS, HTL(ASCP)

 

St. Luke's Hospital

901 East 1st Street

Duluth, Mn 55805

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[Histonet] p16 protocol for VMS instruments

[Sebree Linda A]

For another method that works on Ventana XTs and BenchMarks:

BD antibody (cat.#: 550834), 1:10, mild CC1, 32  incubation @ 42
degrees, Hem II/8 


Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark
Turner
Sent: Thursday, February 24, 2011 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Several of you have asked that I share our protocol for the P16 on the
Ventana XT.  I apologize for the delay, but here is what we have worked
up and it performs well in our lab:

CC1 Standard
Antibody incubation @ 37 degrees C. for 28 minutes
Amplification
Hematoxylin counterstain 4 minutes
Bluing Reagent 4 minutes

We place the commercial antibody from the CINtec kit in a prep kit and
use it as an RTU.


Mark A. Turner, Ph.D. HT (ASCP) QIHC  |  Test Development Team Lead |
Department of Laboratory  Medicine and Pathology |  480-301-8026 |  Fax:
480-301-8372  | turner.m...@mayo.edu
Mayo Clinic in Arizona  |  13400 E. Shea Blvd.  |  Scottsdale, AZ 85259
|  www.mayoclinic.org

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[Histonet] p16 antibody

[Michele Carr]

Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM?
Thanks in advance.
Michele Carr HTL ASCP
Medical Laboratory Services



  
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Re: [Histonet] p16 antibody

[Victoria Baker]

Yes.  They hold the patent.
On Feb 10, 2011 1:30 PM, Michele Carr michelecar...@yahoo.com wrote:
 Hi everyone was wondering who carries the IVD p16 antibody? Is it only
MTM?
 Thanks in advance.
 Michele Carr HTL ASCP
 Medical Laboratory Services




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RE: [Histonet] p16 antibody

[Walter Benton]

 MTM has the patent rights so they are the only one.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr 
[michelecar...@yahoo.com]
Sent: Thursday, February 10, 2011 1:29 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 antibody

Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM?
Thanks in advance.
Michele Carr HTL ASCP
Medical Laboratory Services




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Re: [Histonet] p16 antibody

[Mark Turner]

They hold the patent and no one else produces it.  We order the kit and use the 
antibody on the Ventana XT stainers, placing the antibody in a prep kit.  Works 
great.

Mark Turner, PhD, HT(ASCP)QIHC
Mayo Clinic in Arizona


 Michele Carr michelecar...@yahoo.com wrote: 
 Hi everyone was wondering who carries the IVD p16 antibody? Is it only MTM?
 Thanks in advance.
 Michele Carr HTL ASCP
 Medical Laboratory Services
 
 
 
   
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[Histonet] p16 antibody

[Michele Carr]

Hello all I am relatively new to IHC and my pathologist is requesting the p16 
antibody, I have not been able to find it at any of the vendors we work with. I 
did a google search and saw that MTM labs has it but it seems as though they 
sell it as a kit. Does anyone know where I could find this antibody that I 
could 
use on the bond autostaining machine. Thank you,
Michele Carr HTL ASCP
Medical Laboratory Services
Murrieta Ca


  
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RE: [Histonet] p16 antibody

[McMahon, Loralee A]

If you want the IVD form of the antibody you have to go with MTM labs, they 
hold the patent.  We buy the kit and take out the antibody and use the 
detection for research.   It works very very well.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr 
[michelecar...@yahoo.com]
Sent: Thursday, October 07, 2010 1:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 antibody

Hello all I am relatively new to IHC and my pathologist is requesting the p16
antibody, I have not been able to find it at any of the vendors we work with. I
did a google search and saw that MTM labs has it but it seems as though they
sell it as a kit. Does anyone know where I could find this antibody that I could
use on the bond autostaining machine. Thank you,
Michele Carr HTL ASCP
Medical Laboratory Services
Murrieta Ca



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RE: [Histonet] p16 antibody

[Settembre, Dana]

Hi Michele,
Yes, it's true, only MTM labs sells it and they
Sell it as a kit and they also 
sell it along with a negative control reagent.
That's how I buy it.
They have a license or something and no else can sell it now.
Their cat# is 9518 and I think it's called the 
CINteck Histology V-kit,
(you should double check with them.)
They will make you open up an account...

Good Luck
Dana Settembre
University Hospital - UMDNJ
Newark, NJ



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Carr
Sent: Thursday, October 07, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 antibody

Hello all I am relatively new to IHC and my pathologist is requesting the p16 
antibody, I have not been able to find it at any of the vendors we work with. I 
did a google search and saw that MTM labs has it but it seems as though they 
sell it as a kit. Does anyone know where I could find this antibody that I 
could 
use on the bond autostaining machine. Thank you,
Michele Carr HTL ASCP
Medical Laboratory Services
Murrieta Ca


  
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Re: [Histonet] p16

[Dana Settembre]

Hi Deanne,
My understanding is that you must get p16 from MTM Laboratories.  They
have some sort of monopoly right now. I still have some leftover from
Cell Marque and when I run low or the antibody is about to expire I must
also go to MTM.

I currently use the Cell Marque p16 Ready To Use by pretreating with
Dako's TRS for 40min in steamer, and detect with labelled polymer.
Dako's Envision + Mouse kit. It works nicely.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJUSA


 Knutson, Deanne dknut...@primecare.org 03/09/10 5:27 PM 
Hello fellow Histonetters,

Does anyone run the antibody p16?  If so, where do you purchase it from
and maybe you can share your protocol with me?  Thank you in advance.

Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
 (701)-530-6730
dknut...@primecare.orgmailto:dknut...@primecare.org



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RE: [Histonet] p16

[Cynthia Pyse]

We purchase our p16 through MTM laboratories reference # 9517.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson,
Deanne
Sent: Tuesday, March 09, 2010 5:28 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] p16

Hello fellow Histonetters,

Does anyone run the antibody p16?  If so, where do you purchase it from and
maybe you can share your protocol with me?  Thank you in advance.

Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
 (701)-530-6730
dknut...@primecare.orgmailto:dknut...@primecare.org



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of the message.

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[Histonet] p16

[Knutson, Deanne]

Hello fellow Histonetters,

Does anyone run the antibody p16?  If so, where do you purchase it from and 
maybe you can share your protocol with me?  Thank you in advance.

Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
 (701)-530-6730
dknut...@primecare.orgmailto:dknut...@primecare.org



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intended for your use, please destroy immediately and contact the sender of the 
message.

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[Histonet] p16

[godsgalnow]

Anyone know where I can get an IVD p16?

Roxanne
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