Re: the absorbance value 1.05 at 260/280 of protein solution
Hi all Thank you very much for the replies. On Wed, Oct 28, 2015 at 7:40 PM, Nair, Jayakumar < r.jayaku...@roswellpark.org> wrote: > Nothing if you are measuring only protein concentration. A Lot if you > were measuring DNA concentration. > Jay > > > -Original Message- > From: methods-boun...@oat.bio.indiana.edu [mailto: > methods-boun...@oat.bio.indiana.edu] On Behalf Of Sudheer Sangeetham > Sent: Wednesday, October 28, 2015 7:14 AM > To: meth...@oat.bio.indiana.edu > Subject: the absorbance value 1.05 at 260/280 of protein solution > > Hello people > > What does it mean by the absorbance value 1.05 at 260/280 while measuring > the protein concentration at 280 nm ? > > Thank you in advance > -- > Sudheer Babu.S > Research Fellow > Institute of Biochemistry > Biological Research Center > Szeged,Hungary. > ___ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > > > This email message may contain legally privileged and/or confidential > information. If you are not the intended recipient(s), or the employee or > agent responsible for the delivery of this message to the intended > recipient(s), you are hereby notified that any disclosure, copying, > distribution, or use of this email message is prohibited. If you have > received this message in error, please notify the sender immediately by > e-mail and delete this email message from your computer. Thank you. -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
Re: the absorbance value 1.05 at 260/280 of protein solution
It means you have to get a good text book on spectrophotometry and read it. That will give you a good idea of what exactly it means. :) Good luck! Pow On 28 October 2015 at 07:13, Sudheer Sangeetham wrote: > Hello people > > What does it mean by the absorbance value 1.05 at 260/280 while measuring > the protein concentration at 280 nm ? > > Thank you in advance > -- > Sudheer Babu.S > Research Fellow > Institute of Biochemistry > Biological Research Center > Szeged,Hungary. > ___ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
RE: the absorbance value 1.05 at 260/280 of protein solution
Nothing if you are measuring only protein concentration. A Lot if you were measuring DNA concentration. Jay -Original Message- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Sudheer Sangeetham Sent: Wednesday, October 28, 2015 7:14 AM To: meth...@oat.bio.indiana.edu Subject: the absorbance value 1.05 at 260/280 of protein solution Hello people What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ? Thank you in advance -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
RE: the absorbance value 1.05 at 260/280 of protein solution
You can use the A260/A280 as an indicator of protein purity. Ideally, pure protein should have an A260/A280 value of 0.6, so 1.05 is very high, and indicates that you have nucleic acid contamination (the A260 component is too high). From: methods-boun...@oat.bio.indiana.edu [methods-boun...@oat.bio.indiana.edu] on behalf of Sudheer Sangeetham [sudheer.pb...@gmail.com] Sent: Wednesday, October 28, 2015 7:13 AM To: meth...@oat.bio.indiana.edu Subject: the absorbance value 1.05 at 260/280 of protein solution Hello people What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ? Thank you in advance -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods ___ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
