[spctools-discuss] Query related to Lorikeet viewer PTMprophet results in TPP
Hello Sir/Madam, We used TPP and PTMprophet for PTM analysis. PTM taken (Phosphorylation- STY). *I want to see the spectra in Lorikeet viewer but it is showing following error.* [image: image.png] Please help me solve this. Looking forward to hearing from you. Thanks and regards, *Mehar Un Nissa* Research Scholar, Proteomics Lab. Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay-400076 -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CAH%2BQocr%2B1qCh4xPeieoWE3DZjzqgxcqX%3DFcutxKVe8NHp03X-A%40mail.gmail.com.
[spctools-discuss] irt issue
Hi, I have a pepxml from iprophet combining results from Mascot/X!Tandem and MSGF+. When I am trying to create a spectral library using Spectrast with the following command: spectrast -cNspeclib -cICID-QTOF -cP0.814339 -c_IRTirt.txt -Lspectrastlog_hek.log mascot_xtandem_msgfp_combine.pep.xml I get and error log which says RT inversion detected at iRT 68.168 for some files and then it creates an empty splib file. Can you tell me what does this error exactly mean? ERROR PEPXML IMPORT: RT normalization by linear interpolation. Found 21 landmarks in MS run "170825_pc4_hek_2dcon_13". RT inversion detected at iRT = 68.8618. No RT normalization performed. Also, does spectrast support mzml or we always need mzXML? Regards, Srikanth -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/4af60e36-ef5b-4835-95a7-9f3b1f2b65e1%40googlegroups.com.
