Re: [aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-16 Thread Henrik Bengtsson
On Mon, Dec 16, 2013 at 12:03 PM, Emilie emilie.lalo...@gmail.com wrote: Yep, I think you're right, for this sample there are many points away from the y=x line. https://lh4.googleusercontent.com/-rLT39dQUYe0/Uq9aLgEKsPI/KWY/Uv1cJZGoVTo/s1600/2013-12-16_KB170TvsKB170B_beta_TvsN.png

Re: [aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-13 Thread Henrik Bengtsson
On Fri, Dec 13, 2013 at 11:15 AM, Emilie emilie.lalo...@gmail.com wrote: Thanks again Henrick. I do see 3 bands, but not sure they are necessarily clean/distinct. https://lh5.googleusercontent.com/-yPngBXg2loA/Uqtcqi2z4YI/KWI/mcUOS0DWiyQ/s1600/2013-12-13_KB170B_BAF.png These normal

Re: [aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-05 Thread Henrik Bengtsson
Pierre beat me to this one. Comments below... On Thu, Dec 5, 2013 at 9:20 AM, Pierre Neuvial pierre.neuv...@genopole.cnrs.fr wrote: Hi Emilie, OK, so you are referring to the “smooth.CNA function in the DNAcopy package, cf

Re: [aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-04 Thread Emilie
Hi Pierre, Thanks for your answer. I may be wrong but I thought smoothing prior to segmentation was somewhat common. It is shown in the vignettes for DNACopy and seems to be fairly common in the literature (this approach was used in the Metabric paper for example,

[aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-03 Thread Emilie
Hi there, I'm new to processing Affy SNP6 chips and so am mainly experimenting with different methods to date. I ran CRMAv2 and followed steps 1-4 from the vignette (http://aroma-project.org/vignettes/CRMAv2). For step 5, I want to do a paired analysis. Previously I've used DNAcopy to perform

Re: [aroma.affymetrix] smoothing after CRMAv2 and before CBS?

2013-12-03 Thread Pierre Neuvial
Hi Emilie, It's certainly possible to do this within the Aroma framework (e.g. using the function binnedSmoothing). It's probably not as straightforward as running the segmentation directly, though, because this is not a typical use case. In fact, I'm not sure why you want to perform smoothing