Re: [Artemis-users] ACT comparisons

2011-02-16 Thread Bossers, Alex
Magdalen, not sure if its supported but we successfully use MUMmer locally (or blast+) to generate the required comparison files. Best wishes, Alex Van: artemis-users-boun...@sanger.ac.uk [artemis-users-boun...@sanger.ac.uk] namens Magdalen Lindeberg [m...@cornel

[Artemis-users] ACT comparisons

2011-02-16 Thread Magdalen Lindeberg
After years of using the "Generate" feature at WebACT http://www.webact.org/WebACT/generate for generating ACT readable comparison files, my recent attempts to generate comparisons with this feature have not been successful (basically, the comparison generator never concludes the job). Fortuna

Re: [Artemis-users] multiple contigs, blast and interproscan

2011-02-16 Thread Tim Carver
Hi Intikhab Artemis can read in the output from blastall when it is run with the Œ-m 8¹ flag to generate the one line per HSP. It then displays each blast HSP as a separate feature. The blast needs to be run on the single fasta sequence of the contigs (rather than a multiple fasta) so that the coo

[Artemis-users] multiple contigs, blast and interproscan

2011-02-16 Thread Dr. Intikhab Alam
Hi, I've got contigs from an assembly where I predicted ORFs and performed blasts against NR, UNIprot etc and also perfomed interproscans. Is there a way to correctly load these results into Artemis? I tried loading all multiple sequences and GFF files with co-ordinates on the genomic context, it