[base] Dealing wiht duplicate spots

2011-11-21 Thread Nantel Andre
Greetings, We are currently trying to integrate BASE into our lab and writing the necessary plug-ins to import normalized data from ImaGene. Let's just say that the lack of documentation has been "challenging". Right now we are trying to figure out how to deal with duplicate spots. From the Ge

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nicklas Nordborg
On 2011-11-21 17:08, Nantel Andre wrote: > Greetings, > > We are currently trying to integrate BASE into our lab and writing the > necessary plug-ins to import normalized data from ImaGene. Let's just > say that the lack of documentation has been "challenging". > > Right now we are trying to figure

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nantel Andre
That issue goes beyond the ImaGene format. When doing contact printing of microarrays it is still fairly common to spot the same probe 2 or more times as shown in the example here (http://www.digitalapoptosis.com/2005/10/12/dna-microarray/). It is also very common for commercial arrays (Agilent

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nicklas Nordborg
On 2011-11-21 19:23, Nantel Andre wrote: > That issue goes beyond the ImaGene format. When doing contact > printing of microarrays it is still fairly common to spot the same > probe 2 or more times as shown in the example here > (http://www.digitalapoptosis.com/2005/10/12/dna-microarray/). > > It i

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nantel Andre
On 2011-11-21, at 2:13 PM, Nicklas Nordborg wrote: > The demo data set is a GenePix data set and for each spot the file > contains the coordinates, reporter id and a lot of measured intensities > and other values. So if your data is similar to this, then I think you > don't need any special new

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nicklas Nordborg
On 2011-11-21 20:31, Nantel Andre wrote: > On 2011-11-21, at 2:13 PM, Nicklas Nordborg wrote: > >> The demo data set is a GenePix data set and for each spot the file >> contains the coordinates, reporter id and a lot of measured intensities >> and other values. So if your data is similar to this, t

Re: [base] Dealing wiht duplicate spots

2011-11-21 Thread Nantel Andre
On 2011-11-21, at 2:51 PM, Nicklas Nordborg wrote: > On 2011-11-21 20:31, Nantel Andre wrote: >> On 2011-11-21, at 2:13 PM, Nicklas Nordborg wrote: >> >>> The demo data set is a GenePix data set and for each spot the file >>> contains the coordinates, reporter id and a lot of measured intensitie