Hi Mathews
One of our customers, Bret Dillard at the University of Georgia, has
had great success with one of our robots in a Bactron X anaerobic
chamber.
In this case, Bret mainly used the microbatch-under-oil method, which
works very well for anaerobic work: (1) the oil protects the sample
Ronaldo
I have a database of crystallization conditions extracted from the
PDB. I was able to parse the data to extract the concentration of
protein in around 900 cases.
The lowest 9 protein concentrations were as follows:
PDB ID TYPEDATEProtein conc, mg/ml
1EYMISOMERASE.
Dear Patrick,
as a (probably more general) follow up-question:
Does anyone have experience with batch crystallization under oil for membrane
proteins (in detergent) ?
alex
Dr. Alexander Pautsch
Protein Crystallography /Structural Research
Boehringer Ingelheim Pharma GmbH Co. KG
Hi Mathews,
An anaerobic chamber from Belle Technology (http://www.belle-
technology.com/) served me well in the past.
Belle's glove boxes are made of acrylic material. That offers
relatively low-cost and all-round visibility.
Cheers,
Roberto
On 23 Feb 2007, at 02:03, Mathews, Irimpan
Dear Mathews,
we were successful in crystallizing two ferredoxins under strict anaerobic
conditions using the much cheaper solution of a glove bag and filling it
with argon. I guess that in both cases (box and bag) the thickness of the
gloves is a problem especially if you are dealing with
Just to add that in one case we were able to get suitable crystals after using a
protein concentration of as low as 1.5 mg/ml (kapetaniou et al. Acta Cryst.
2005, F61, 479-481) and microdialysis to slowly remove the excess of salt.
Lower concentrations (0.50-0.75 mg/ml) could also produce crystals
Hello all,
Kindly disregard my message about monomer libraries. The problem ( atoms
blowing apart) seems to be due to something (yet undetermined) else. Thank
you for clearing up ones about COM_COM.
However, I still wonder if I can add my own ligand libraries to the
default one, like you can do
Hi, David Borhani is no longer with Abbott. Please resend your email to:
[EMAIL PROTECTED]
