Dear all,
Sorry for the non-CCP4 question.
We recently crystallized a protein expressed from pGEX4T-1 vector. The GST tag
is cleaved by thrombin before crystallization. The cleavage site of thrombin is
Leu-Val-Pro-Arg || Gly-Ser, thus Gly and Ser should remain at the N-terminus of
the protein
I've used cheap Peltier incubators in the past (when funds were scarse) and
they were just fine - with the caveat that the incubators I used were small
-- basically they were portable beer coolers with a temperature controller
(made by Koolatron). First thing I did was to check the temperature and
Hi all,
I have a question about registering Se.
Since diffraction quality of my SeMet dataset is better than a native one, I
want to use a SeMet dataset (collected at a remote wavelength) for a final
model while I am trying to get a decent native dataset. I tried to put MSE in
some MET positio
That is exactly how it is supposed to work with 10.5. No need to use
an xterm, and it sets the DISPLAY variable automatically. I assume
you have write-permissions in the directory it is trying to make
the .CCP4 directory?
On Mar 25, 2008, at 9:32 AM, Kurt Padilla wrote:
Sue,
Currently
Dear All:
I want to draw your attention to a very useful server for
checking for new folds etc...
Thx, br
-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of
Markus Wiederstein
Sent: Tuesday, March 25, 2008 6:56 AM
To: [EMAIL PROTECTED]
Subject: pdb-l: Top
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
This is a 10.5 feature : when an application tries to connect to the
X11 window server, X11 is automatically launched (unless it was
already running). Hence in Leopard you can call ccp4i from within
Terminal.app without much more to setup than t
The Institute of Molecular Biology at the University of Oregon (www.molbio.uoregon.edu
) has an opening for a macromolecular crystallography facilities
manager. The successful candidate will be responsible for maintaining
a rotating anode source, area detector and computational facilities to
Sue,
Currently, when I type in ccp4i into Terminal, X11 launches and then CCP4
does too. I am running OS X 10.5 with a fink installation.
Kurt
On Tue, Mar 25, 2008 at 12:04 PM, Sue Roberts <[EMAIL PROTECTED]>
wrote:
> I also thought the problem was using the Terminal window and not the
> X11 wi
I also thought the problem was using the Terminal window and not the
X11 window, but, interestingly, typing ccp4i in a Terminal window,
not an X11 window does work.
X11 does need to be installed and running, -- that could be the problem.
(Caveat, this is in 10.4, not 10.5 - I have 10.5 but ha
Hi -
You need to add to your .chrc, or type in an ***X11*** window (and
not in the Terminal).
source /usr/local/ccp4-6.0.2//bin/ccp4.setup-csh
then it will work.
it does not really install a real application in the OSX sense.
A.
On Mar 25, 2008, at 15:47, Kurt Padilla wrote:
Wil
Hi Kurt,
the CCP4 program package for Mac OS X from the CCP4 download site
installs the CCP4 suite under /usr/local or any other path that you
provide during installation. There is no such thing as a CCP4.app that
can be started by clicking on it. Instead, you have to create in a
Terminal
In both cases, you have to make sure you are setting up the
environment appropriately for ccp4, unique to the unix shell you are
using.
In the case of the ccp4 package you compile with the aid of the fink
script, at the start of a shell session, you, or your dot file, needs
to issue
sou
HI Simon:
The X11 guy at Apple is very interested in trying to help. If you
could join the Apple X11 mailing list and document this as carefully
as possible, it would be a real benefit. He's out of town for a few
days but is interested in dealing with this as soon as he returns.
There is
William,
Thanks for your advice. I intend to give it a try in the next few days.
Hopefully, I will have some positive results to share.
Anastassis,
I tried using the binary from the link you provided, but I was unable to get
the program to run even though Installer said that the installation was
Hi -
I am sure that its not a fink problem really, but why not try the
CCP4 distribution?
http://www.ccp4.ac.uk/download/
A.
On Mar 22, 2008, at 5:34, Kurt Padilla wrote:
Hello,
When I click on 'Apply' in the Directories & Project Directory
window, I get the following error:
ERROR sa
Has anyone found a solution to the X window problem on macs running
10.5.2? Both Coot and pymol are becoming almost unusable by freezing
frequently because of X11 "not responding". I've upgraded my X11 to
2.1.4 from the webpage http://trac.macosforge.org/projects/xquartz/
wiki/ but the probl
Dear all,
This is a reminder that March 31, 2008 is the deadline for applying to
the EMBO
Practical Course on the Structural Characterization of Macromolecular
Complexes
to be held in Grenoble, France during the week of June 2-7, 2008.
TOPICS INCLUDE:
- expression and purification of multi-s
There will be a workshop on NMR and X-Ray in Structural Biology
to be held at IECB (Bordeaux FRANCE) on 14 April 2008.
Invited Speakers :
Franck Artzner (Rennes), Martin Blackledge (Grenoble), Rolf Boelens
(Utrecht), Yves Bourne (Marseille), Antoinette Killian (Utrecht),
Jean-Yves Lallemand (Gif-s
Dear all,
Has anyone got experience with Peltier Crystallisation Incubators?
According to manufacturers' info they should be OK for small volumes.
I have some doubts regarding their effieciency when you open one say
ten times a day for one minute how quick they cope with it.
Any ideas?
Thanks
Jan
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